AB190285

PerCP/Cy5.5® Anti-CD3 epsilon antibody [145-2C11]

Name: Anti-CD3 epsilon PerCP/Cy5.5® antibody [145-2C11] (ab190285) IgG | Abcam
Brand: Abcam Limited
SKU: AB190285

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(2 Publications)

Armenian hamster Monoclonal CD3E antibody - conjugated to PerCP/Cy5.5®. Suitable for Flow Cyt and reacts with Mouse samples. Cited in 2 publications. Immunogen corresponding to Cell preparation containing Cd3e protein.

View Alternative Names

CD3e, T-cell surface glycoprotein CD3 epsilon chain, T-cell surface antigen T3/Leu-4 epsilon chain, Cd3e

1 Images

Flow Cytometry - PerCP/Cy5.5® Anti-CD3 epsilon antibody [145-2C11] (AB190285)

Flow Cyt

Supplier Data

Flow Cytometry - PerCP/Cy5.5® Anti-CD3 epsilon antibody [145-2C11] (AB190285)

Flow cytometry analysis of mouse splenocytes labeling CD3 epsilon using ab190285 at 3 μg/mL (Red) as compared to unstained cells (Blue). Surface staining.

Carrier free

Anti-CD3 epsilon antibody [145-2C11] - Low endotoxin, Azide free
675 PerCP

PerCP Anti-CD3 epsilon antibody [145-2C11]
660 APC

APC Anti-CD3 epsilon antibody [145-2C11]

Key facts

Host species

Armenian hamster

Clonality

Monoclonal

Clone number

145-2C11

Isotype

IgG

Conjugation

PerCP/Cy5.5®

Excitation/Emission

Ex: 482nm, Em: 695nm

Carrier free

Reacts with

Mouse

Applications

Flow Cyt

applications

Immunogen

Cell preparation containing Cd3e protein. The exact immunogen used to generate this antibody is proprietary information.

P22646

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1-3 µg/mL", "FlowCyt-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Size-exclusion chromatography

Purification notes

Purified antibody is conjugated with tandem dye PerCP-Cy™5.5 under optimum conditions. The conjugate is purified by size-exclusion chromatography.

Storage buffer

pH: 7.4 Preservative: 0.097% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Store in the dark

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways. In addition of this role of signal transduction in T-cell activation, CD3E plays an essential role in correct T-cell development (PubMed : 19956738, PubMed : 24899501). Participates also in internalization and cell surface down-regulation of TCR-CD3 complexes via endocytosis sequences present in CD3E cytosolic region. In addition to its role as a TCR coreceptor, it serves as a receptor for ITPRIPL1. Ligand recognition inhibits T-cell activation by promoting interaction with NCK1, which prevents CD3E-ZAP70 interaction and blocks the ERK-NFkB signaling cascade and calcium influx (By similarity).

See full target information Cd3e

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 21: PubMed32957694

2020

Microglia-Astrocyte Communication via C1q Contributes to Orofacial Neuropathic Pain Associated with Infraorbital Nerve Injury.

Applications

Unspecified application

Species

Unspecified reactive species

Sayaka Asano,Yoshinori Hayashi,Koichi Iwata,Akiko Okada-Ogawa,Suzuro Hitomi,Ikuko Shibuta,Yoshiki Imamura,Masamichi Shinoda

Experimental and therapeutic medicine 16:1403-1410 PubMed30112067

2018

Effect of TGF-β1 on blood CD4CD25 regulatory T cell proliferation and Foxp3 expression during non-small cell lung cancer blood metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

Yi Hu,Weibo Qi,Li Sun,Hui Zhou,Biliu Zhou,Zhiping Yang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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PerCP/Cy5.5® Anti-CD3 epsilon antibody [145-2C11]

Flow Cytometry - PerCP/Cy5.5® Anti-CD3 epsilon antibody [145-2C11] (AB190285)

Related conjugates and formulations (3)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Publications (2)

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