JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB210265

PerCP/Cy5.5® Anti-CD4 antibody [GK1.5]

Be the first to review this product! Submit a review

|

(2 Publications)

Rat Monoclonal CD4 antibody - conjugated to PerCP/Cy5.5®. Suitable for Flow Cyt and reacts with Mouse samples. Cited in 2 publications.

View Alternative Names

CD4, T-cell surface glycoprotein CD4, T-cell differentiation antigen L3T4, T-cell surface antigen T4/Leu-3, Cd4

1 Images
Flow Cytometry - PerCP/Cy5.5® Anti-CD4 antibody [GK1.5] (AB210265)
  • Flow Cyt

Supplier Data

Flow Cytometry - PerCP/Cy5.5® Anti-CD4 antibody [GK1.5] (AB210265)

Flow cytometric analysis of C57Bl/6 splenocytes labeling CD4 with 0.06 μg ab210265 (solid line), compared with 0.06 μg Per-Cy5.5 Rat IgG2b isotype control (dashed line).

  • 660 APC

    APC Anti-CD4 antibody [GK1.5]

  • 519 FITC

    FITC Anti-CD4 antibody [GK1.5]

  • 519 FITC

    FITC Anti-CD4 antibody [GK1.5]

  • 667 PE/Cy5®

    PE/Cy5® Anti-CD4 antibody [GK1.5]

  • 704 redFluor™ 710

    redFluor™ 710 Anti-CD4 antibody [GK1.5]

  • 450 violetFluor™ 450

    violetFluor™ 450 Anti-CD4 antibody [GK1.5]

Key facts

Host species

Rat

Clonality

Monoclonal

Clone number

GK1.5

Isotype

IgG2b

Light chain type

kappa

Conjugation

PerCP/Cy5.5®

Excitation/Emission

Ex: 482nm, Em: 695nm

Carrier free

No

Reacts with

Mouse

Applications

Flow Cyt

applications

style
left-column

Alternative Products

Primary Antibodies

AB133616

Anti-CD4 antibody [EPR6855]

BOND RX™ Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size|Advanced Validation

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD4 antibody [EPR6855] (AB133616)

  • 5
  • 20
Primary Antibodies

AB183685

Anti-CD4 antibody [EPR19514]

RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD4 antibody [EPR19514] (AB183685)

  • 5
  • 21
Primary Antibodies

AB196147

Alexa Fluor® 647 Anti-CD4 antibody [EPR6855]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-CD4 antibody [EPR6855] (AB196147)

  • 5
  • 1
Primary Antibodies

AB196372

Alexa Fluor® 488 Anti-CD4 antibody [EPR6855]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-CD4 antibody [EPR6855] (AB196372)

  • 4
  • 2
Primary Antibodies

AB221775

Anti-CD4 antibody [EPR19514] - Low endotoxin, Azide free

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD4 antibody [EPR19514] - Low endotoxin, Azide free (AB221775)

  • 0
  • 0
Primary Antibodies

AB238798

Anti-CD4 antibody [SP35] - BSA and Azide free

BOND RX™ Validated|Recombinant|Advanced Validation|RabMAb

Multiplex immunohistochemistry - Anti-CD4 antibody [SP35] - BSA and Azide free (AB238798)

  • 0
  • 0
Primary Antibodies

AB277931

Alexa Fluor® 594 Anti-CD4 antibody [EPR6855]

BOND RX™ Validated|RabMAb|Recombinant|Trial Size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 594 Anti-CD4 antibody [EPR6855] (AB277931)

  • 0
  • 0

Complementary Products

Assay Kits

AB234569

Human CD4 ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human CD4 ELISA Kit (AB234569)

  • 0
  • 0
Primary Antibodies

AB237365

Alexa Fluor® 647 Anti-CD8 alpha antibody [EPR21769]

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-CD8 alpha antibody [EPR21769] (AB237365)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB34722

FITC Anti-CD3 antibody [CD3-12]

Lab Essentials

  • 0
  • 0
Primary Antibodies

AB200317

Alexa Fluor® 647 Anti-CD45 antibody [EP322Y]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-CD45 antibody [EP322Y] (AB200317)

  • 0
  • 1
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p>Use 0.06 μg.</p>" }, "Syrian hamster": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Purification notes
ab210265 was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.
Storage buffer
pH: 7.2 Preservative: 0.09% Sodium azide Constituents: 0.87% Sodium chloride, 0.12% Sodium dihydrogen phosphate, 0.1% Gelatin
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Store in the dark
style
left-column
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class II molecule : peptide complex. The antigens presented by class II peptides are derived from extracellular proteins while class I peptides are derived from cytosolic proteins. Interacts simultaneously with the T-cell receptor (TCR) and the MHC class II presented by antigen presenting cells (APCs). In turn, recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex. LCK then initiates different intracellular signaling pathways by phosphorylating various substrates ultimately leading to lymphokine production, motility, adhesion and activation of T-helper cells. In other cells such as macrophages or NK cells, plays a role in differentiation/activation, cytokine expression and cell migration in a TCR/LCK-independent pathway. Participates in the development of T-helper cells in the thymus and triggers the differentiation of monocytes into functional mature macrophages.
See full target information Cd4
style
left-column

Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Environmental toxicology 38:146-158 PubMed36181686

2022

Blimp1 suppressed CD4 T cells-induced activation of fibroblast-like synoviocytes by upregulating IL-10 via the rho pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Qingliang Meng,Zhike Wen,Wanting Meng,Hua Bian,Huimin Gu,Ruiting Zuo,Junping Zhan,Huilian Wang,Xiyun Miao,Wei Fan,Zipeng Zhou,Fuzeng Zheng,Liying Wang,Xiao Su,Junfu Ma

Nature cancer 3:337-354 PubMed35256819

2022

An anti-PD-1-GITR-L bispecific agonist induces GITR clustering-mediated T cell activation for cancer immunotherapy.

Applications

Unspecified application

Species

Unspecified reactive species

Sarah Chan,Nicole Belmar,Sun Ho,Bryan Rogers,Marcia Stickler,Michelle Graham,Eileen Lee,Ninian Tran,Dong Zhang,Priyanka Gupta,Mien Sho,Tracy MacDonough,Andrew Woolley,Han Kim,Hong Zhang,Wei Liu,Pingping Zheng,Zoltan Dezso,Kyle Halliwill,Michele Ceccarelli,Susan Rhodes,Archana Thakur,Charles M Forsyth,Mengli Xiong,Siu Sze Tan,Ramesh Iyer,Marc Lake,Enrico Digiammarino,Li Zhou,Lance Bigelow,Kenton Longenecker,Russell A Judge,Cassie Liu,Max Trumble,Jonathan P Remis,Melvin Fox,Belinda Cairns,Yoshiko Akamatsu,Diane Hollenbaugh,Fiona Harding,Hamsell M Alvarez
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com