AB210373

PerCP/Cy5.5® Anti-CD4 antibody [SK3]

Name: Anti-CD4 PerCP/Cy5.5® antibody [SK3] (ab210373) IgG1 | Abcam
Brand: Abcam Limited
SKU: AB210373
Price: 265 USD
Availability: InStock

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(2 Publications)

Mouse Monoclonal CD4 antibody - conjugated to PerCP/Cy5.5®. Suitable for Flow Cyt and reacts with Human samples. Cited in 2 publications.

View Alternative Names

CD4, T-cell surface glycoprotein CD4, T-cell surface antigen T4/Leu-3

1 Images

Flow Cytometry - PerCP/Cy5.5® Anti-CD4 antibody [SK3] (AB210373)

Flow Cyt

Supplier Data

Flow Cytometry - PerCP/Cy5.5® Anti-CD4 antibody [SK3] (AB210373)

Flow cytometric analysis of Human peripheral blood lymphocytes labeling CD4 with 5 μl (0.06 μg) ab210373 (solid line), compared with 0.06 μg PerCP/Cy5.5 Mouse IgG1 isotype control (dashed line).

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

SK3

Isotype

IgG1

Light chain type

kappa

Conjugation

PerCP/Cy5.5®

Excitation/Emission

Ex: 482nm, Em: 695nm

Carrier free

Reacts with

Human

Applications

Flow Cyt

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "5 µL for 10^5-10^8 Cells", "FlowCyt-species-notes": "<p>The antibody has been diluted for use at 5 μl (0.06 μg) per test, defined as the amount of antibody that will stain a cell sample in a final volume of approximately 100 μl.</p>" }, "Cynomolgus monkey": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" }, "Rhesus monkey": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" } } }

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Product details

This antibody does not block binding of alternative clone RPA-T4, suggesting that they recognize different epitopes.

This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5, 268, 486 and related patents. Cy® and CyDye® are trademarks of Cytiva.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Purification notes

ab210373 was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.

Storage buffer

pH: 7.2 Preservative: 0.09% Sodium azide Constituents: 0.87% Sodium chloride, 0.12% Sodium dihydrogen phosphate, 0.1% Gelatin

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Store in the dark

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class II molecule : peptide complex. The antigens presented by class II peptides are derived from extracellular proteins while class I peptides are derived from cytosolic proteins. Interacts simultaneously with the T-cell receptor (TCR) and the MHC class II presented by antigen presenting cells (APCs). In turn, recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex. LCK then initiates different intracellular signaling pathways by phosphorylating various substrates ultimately leading to lymphokine production, motility, adhesion and activation of T-helper cells. In other cells such as macrophages or NK cells, plays a role in differentiation/activation, cytokine expression and cell migration in a TCR/LCK-independent pathway. Participates in the development of T-helper cells in the thymus and triggers the differentiation of monocytes into functional mature macrophages.. (Microbial infection) Primary receptor for human immunodeficiency virus-1 (HIV-1) (PubMed : 12089508, PubMed : 16331979, PubMed : 2214026, PubMed : 9641677). Down-regulated by HIV-1 Vpu (PubMed : 17346169). Acts as a receptor for Human Herpes virus 7/HHV-7 (PubMed : 7909607).

See full target information CD4

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Experimental and therapeutic medicine 25:271 PubMed37206552

2023

Changes in the balance of Th17/Treg cells and oxidative stress markers in patients with HIV‑associated pulmonary tuberculosis who develop IRIS.

Applications

Unspecified application

Species

Unspecified reactive species

Long Wen,Lei Shi,Shan-Shan Wan,Tao Xu,Lei Zhang,Zhi-Guo Zhou

Blood 129:2526-2536 PubMed28351936

2017

Endoglin: a novel target for therapeutic intervention in acute leukemias revealed in xenograft mouse models.

Applications

Unspecified application

Species

Unspecified reactive species

Keina M C Dourado,June Baik,Vanessa K P Oliveira,Miriam Beltrame,Ami Yamamoto,Charles P Theuer,Camila A V Figueiredo,Michael R Verneris,Rita C R Perlingeiro

View all publications

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