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AB210221

PerCP/Cy5.5® Anti-CD45 antibody [2D1]

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(3 Publications )

Anti-CD45 antibody [2D1] conjugated to PerCP/Cy5.5® - suitable for flow cytometry, and reacts with human samples. Clone [2D1] is a mouse monoclonal antibody and one of the most commonly used clones for flow cytometry.

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Flow Cytometry - PerCP/Cy5.5® Anti-CD45 antibody [2D1] (AB210221)
  • Flow Cyt

Supplier Data

Flow Cytometry - PerCP/Cy5.5® Anti-CD45 antibody [2D1] (AB210221)

Flow cytometric analysis of Human PBMCs labeling CD45 with 5 μL (0.125 μg) ab210221 (right panel) or 0.125 μg PerCP-Cy5.5 Mouse IgG1 isotype control (left panel).

  • 723 Alexa Fluor® 700

    Alexa Fluor® 700 Anti-CD45 antibody [2D1]

  • 519 FITC

    FITC Anti-CD45 antibody [2D1]

  • 578 PE

    PE Anti-CD45 antibody [2D1]

  • 778 PE/Cy7®

    PE/Cy7® Anti-CD45 antibody [2D1]

  • 675 PerCP

    PerCP Anti-CD45 antibody [2D1]

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

2D1

Isotype

IgG1

Light chain type

kappa

Conjugation

PerCP/Cy5.5®

Excitation/Emission

Ex: 482nm, Em: 695nm

Carrier free

No

Reacts with

Human

Applications

Flow Cyt

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "5 µL for 10^5-10^8 Cells", "FlowCyt-species-notes": "<p>in a final volume of approximately 100 μL.</p>" } } }
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Product details

The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.

This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5, 268, 486 and related patents. Cy® and CyDye® are trademarks of Cytiva.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Purification notes
ab210221 was purified from tissue culture supernatant via affinity chromatography.
Storage buffer
pH: 7.2 Preservative: 0.09% Sodium azide Constituents: 0.87% Sodium chloride, 0.12% Sodium dihydrogen phosphate, 0.1% Gelatin
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD45 also known as Protein tyrosine phosphatase receptor type C (PTPRC) is a transmembrane glycoprotein with a molecular mass ranging between 180-240 kDa depending on its isoform. It is expressed on the surface of almost all hematopoietic cells except for mature erythrocytes and platelets. CD45 has a critical role in regulating antigen receptor signaling by modifying kinases involved in signal transduction and it is essential in lymphocyte development and activation. Because of its broad expression on immune cells CD45 is a valuable marker for differentiating various immune cell types in assays like flow cytometry and immunohistochemistry (IHC) often referred to as CD45 stain.
Biological function summary

CD45 acts by regulating tyrosine phosphorylation in the immune cell signaling context participating directly in signal transduction. It functions by dephosphorylating specific phosphotyrosine residues on various proteins modulating the signaling threshold required for lymphocyte activation. Although not known to be part of a protein complex CD45 itself shows isoform variation that associates with specific immune cell types impacting their function. Importantly CD45 interacts with multiple signaling molecules to affect cell growth and differentiation.

Pathways

Scientists associate CD45 with signaling pathways such as the T-cell receptor (TCR) and B-cell receptor (BCR) signaling. CD45 modulates the activity of Src family kinases important elements in these pathways making it essential for effective immune response and tolerance. Furthermore interactions between CD45 and proteins like Lck in T-cells or Lyn in B-cells highlight its pivotal role in executing its signaling functions.

Scientists often link CD45 to immune-related conditions including autoimmunity and leukemia. In autoimmune diseases altered CD45 expression or activity can disrupt normal immune function contributing to pathogenesis. In leukemia CD45 expression levels can assist in disease classification and prognosis as it interacts with proteins involved in cell cycle regulation. The anti-CD45 antibodies can provide diagnostic and therapeutic avenues highlighting their utility in disease management.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor (PubMed : 35767951). Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN. Dephosphorylates LYN, and thereby modulates LYN activity (By similarity). Interacts with CLEC10A at antigen presenting cell-T cell contact; CLEC10A on immature dendritic cells recognizes Tn antigen-carrying PTPRC/CD45 receptor on effector T cells and modulates T cell activation threshold to limit autoreactivity.. (Microbial infection) Acts as a receptor for human cytomegalovirus protein UL11 and mediates binding of UL11 to T-cells, leading to reduced induction of tyrosine phosphorylation of multiple signaling proteins upon T-cell receptor stimulation and impaired T-cell proliferation.
See full target information PTPRC

Alternative Names

CD45, PTPRC, Receptor-type tyrosine-protein phosphatase C, Leukocyte common antigen, T200, L-CA
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Publications (3)

Recent publications for all applications. Explore the full listand refine your search

Cell biology and toxicology 41:11 PubMed39707066

2024

Targeting protein tyrosine phosphatase non-receptor type 6 (PTPN6) as a therapeutic strategy in acute myeloid leukemia.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoou Wang,Zhenggang Li,Jing Shen,Lin Liu

Journal of nanobiotechnology 21:459 PubMed38037135

2023

Biomimetic composite hydrogel promotes new bone formation in rat bone defects through regulation of miR-19b-3p/WWP1 axis by loaded extracellular vesicles.

Applications

Unspecified application

Species

Unspecified reactive species

Rongkang Guo,Chaohan Wu,Fan Liu,Tianhua Dong,Tao Zhang

Molecular medicine reports 26: PubMed35856417

2022

MCP‑1/CCR2 axis inhibits the chondrogenic differentiation of human nucleus pulposus mesenchymal stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Xuancheng Ou,Tianyong Wen,Jinwei Ying,Qing He,Anwu Xuan,Dike Ruan
View all publications
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Product promise

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