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Mouse Monoclonal CD45 antibody - conjugated to PerCP/Cy5.5®. Suitable for Flow Cyt and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Cell preparation containing PTPRC protein.


Images

Flow Cytometry - PerCP/Cy5.5® Anti-CD45 antibody [MEM-28] (AB157309), expandable thumbnail
  • Flow Cytometry - PerCP/Cy5.5® Anti-CD45 antibody [MEM-28] (AB157309), expandable thumbnail

Publications

Key facts

Isotype
IgG1
Host species
Mouse
Conjugation
PerCP/Cy5.5®
Excitation/Emission
Ex: 482nm, Em: 695nm
Storage buffer

pH: 7.4
Preservative: 0.1% Sodium azide
Constituents: 99% PBS, 0.2% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • Cell preparation containing PTPRC protein. The exact immunogen used to generate this antibody is proprietary information. Database link P08575

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow Cyt
Human
Tested

Tested
Tested

Species
Human
Dilution info
4 µL for 106 Cells
Notes

Or use 100 μl of whole blood.

ab157226 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Associated Products

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6 products for Alternative Product

Target data

Function

Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor (PubMed:35767951). Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN. Dephosphorylates LYN, and thereby modulates LYN activity (By similarity). (Microbial infection) Acts as a receptor for human cytomegalovirus protein UL11 and mediates binding of UL11 to T-cells, leading to reduced induction of tyrosine phosphorylation of multiple signaling proteins upon T-cell receptor stimulation and impaired T-cell proliferation.

Alternative names

Recommended products

Mouse Monoclonal CD45 antibody - conjugated to PerCP/Cy5.5®. Suitable for Flow Cyt and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Cell preparation containing PTPRC protein.

Key facts

Isotype
IgG1
Conjugation
PerCP/Cy5.5®
Excitation/Emission
Ex: 482nm, Em: 695nm
Form
Liquid
Clonality
Monoclonal
Immunogen
  • Cell preparation containing PTPRC protein. The exact immunogen used to generate this antibody is proprietary information. Database link P08575
Clone number
MEM-28
Purification technique
Size-exclusion chromatography
Specificity

ab157309 reacts with all alternative forms of Human CD45, expressed at high level on all cells of hematopoietic origin, except erythrocytes and platelets.

Concentration
Loading...
Purification notes

ab157309 is conjugated with tandem dye PerCP/Cy5.5® under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary for use in Flow Cytometry studies.

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

CD45 also known as Protein tyrosine phosphatase receptor type C (PTPRC) is a transmembrane glycoprotein with a molecular mass ranging between 180-240 kDa depending on its isoform. It is expressed on the surface of almost all hematopoietic cells except for mature erythrocytes and platelets. CD45 has a critical role in regulating antigen receptor signaling by modifying kinases involved in signal transduction and it is essential in lymphocyte development and activation. Because of its broad expression on immune cells CD45 is a valuable marker for differentiating various immune cell types in assays like flow cytometry and immunohistochemistry (IHC) often referred to as CD45 stain.

Biological function summary

CD45 acts by regulating tyrosine phosphorylation in the immune cell signaling context participating directly in signal transduction. It functions by dephosphorylating specific phosphotyrosine residues on various proteins modulating the signaling threshold required for lymphocyte activation. Although not known to be part of a protein complex CD45 itself shows isoform variation that associates with specific immune cell types impacting their function. Importantly CD45 interacts with multiple signaling molecules to affect cell growth and differentiation.

Pathways

Scientists associate CD45 with signaling pathways such as the T-cell receptor (TCR) and B-cell receptor (BCR) signaling. CD45 modulates the activity of Src family kinases important elements in these pathways making it essential for effective immune response and tolerance. Furthermore interactions between CD45 and proteins like Lck in T-cells or Lyn in B-cells highlight its pivotal role in executing its signaling functions.

Associated diseases and disorders

Scientists often link CD45 to immune-related conditions including autoimmunity and leukemia. In autoimmune diseases altered CD45 expression or activity can disrupt normal immune function contributing to pathogenesis. In leukemia CD45 expression levels can assist in disease classification and prognosis as it interacts with proteins involved in cell cycle regulation. The anti-CD45 antibodies can provide diagnostic and therapeutic avenues highlighting their utility in disease management.

Product promise

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2 product images

  • Flow Cytometry - PerCP/Cy5.5® Anti-CD45 antibody [MEM-28] (ab157309), expandable thumbnail

    Flow Cytometry - PerCP/Cy5.5® Anti-CD45 antibody [MEM-28] (ab157309)

    Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD45 (MEM-28) PerCP-Cy™5.5 antibody (4 µl reagent / 100 µl of peripheral whole blood).

  • Flow Cytometry - PerCP/Cy5.5® Anti-CD45 antibody [MEM-28] (ab157309), expandable thumbnail

    Flow Cytometry - PerCP/Cy5.5® Anti-CD45 antibody [MEM-28] (ab157309)

    Separation of human CD45 positive lymphocytes (red-filled) from CD45 negative blood debris (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD45 (MEM-28) PerCP-Cy™5.5 antibody (4 µl reagent / 100 µl of peripheral whole blood).

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Product protocols

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