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AB272342

PerCP/Cy5.5® Anti-EpCAM antibody [323/A3]

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(1 Publication)

Mouse Monoclonal EPCAM antibody - conjugated to PerCP/Cy5.5®. Suitable for Flow Cyt and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Cell preparation containing EPCAM protein.

View Alternative Names

CD326, GA733-2, M1S2, M4S1, MIC18, TACSTD1, TROP1, EPCAM, Epithelial cell adhesion molecule, Ep-CAM, Adenocarcinoma-associated antigen, Cell surface glycoprotein Trop-1, Epithelial cell surface antigen, Epithelial glycoprotein, Epithelial glycoprotein 314, KS 1/4 antigen, KSA, Major gastrointestinal tumor-associated protein GA733-2, Tumor-associated calcium signal transducer 1, EGP, EGP314, hEGP314

2 Images
Flow Cytometry - PerCP/Cy5.5® Anti-EpCAM antibody [323/A3] (AB272342)
  • Flow Cyt

Supplier Data

Flow Cytometry - PerCP/Cy5.5® Anti-EpCAM antibody [323/A3] (AB272342)

Flow cytometry surface staining pattern of MCF7 (Human breast adenocarcinoma cell line) cell line suspension stained using ab272342 at 4 μl reagent per milion cells in 100 μl of cell suspension. EpCAM also known as CD326.

Flow Cytometry - PerCP/Cy5.5® Anti-EpCAM antibody [323/A3] (AB272342)
  • Flow Cyt

Supplier Data

Flow Cytometry - PerCP/Cy5.5® Anti-EpCAM antibody [323/A3] (AB272342)

Separation of MCF7 (Human breast adenocarcinoma cell line) cells (red-filled) from SP2 cells (black-dashed) in flow cytometry analysis (surface staining) stained using ab272342 at 4 μl reagent per milion cells in 100 μl of cell suspension. EpCAM also known as CD326.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

323/A3

Isotype

IgG1

Conjugation

PerCP/Cy5.5®

Excitation/Emission

Ex: 482nm, Em: 695nm

Carrier free

No

Reacts with

Human

Applications

Flow Cyt

applications

Immunogen

Cell preparation containing EPCAM protein. The exact immunogen used to generate this antibody is proprietary information.

P16422

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p>The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 10<sup>6</sup> cells in a suspension.</p>" } } }
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Product details

This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5, 268, 486 and related patents. Cy® and CyDye® are trademarks of Cytiva.
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Properties and storage information

Form
Liquid
Purification technique
Size-exclusion chromatography
Purification notes
Purified from TCS.
Storage buffer
pH: 7.4 Preservative: 0.0975% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

EpCAM also known as epithelial cell adhesion molecule is a transmembrane glycoprotein with a molecular weight of around 40 kDa. Alternative names include CD326 and TACSTD1. This protein is expressed on the surface of most epithelial cells and plays a role in cell adhesion. It is involved in maintaining the integrity of epithelial tissues by mediating homotypic cell-cell interactions which are important for the structure and function of tissues.
Biological function summary

EpCAM facilitates signaling pathways that impact cellular processes like proliferation and differentiation. It is not a part of a larger protein complex but interacts directly with other cellular machinery to transmit intracellular signals. EpCAM influences stem cell behavior and is important in tissue homeostasis assisting in both normal regenerative processes and wound healing.

Pathways

EpCAM participates in regulatory mechanisms involving the Wnt/β-catenin signaling pathway which affects gene expression and cell fate decisions. Through its action EpCAM modulates components like E-cadherin which is important for cell adhesion and the maintenance of the epithelial layer. It indirectly influences pathways relating to MYC a transcription factor that regulates cell cycle progression and apoptosis.

EpCAM is associated with various types of cancer including colorectal and breast cancer. Its overexpression often correlates with aggressive cancer phenotypes and poor prognosis. Besides cancers EpCAM mutations can lead to congenital tufting enteropathy a rare condition affecting intestinal epithelial structure and function. Aberrant EpCAM expression affects β-catenin levels impacting cellular pathways linked to tumor progression and epithelial integrity.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

May act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. Plays a role in embryonic stem cells proliferation and differentiation. Up-regulates the expression of FABP5, MYC and cyclins A and E.
See full target information EPCAM
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

iScience 27:108957 PubMed38333692

2024

EP300 restores the glycolytic activity and anti-tumor function of CD8 cytotoxic T cells in nasopharyngeal carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Zhixiu Xia,Xiaoxu Ding,Chao Ji,Dabo Zhou,Xun Sun,Tiancong Liu
View all publications
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Product promise

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