AB234263

PerCP/Cy5.5® Anti-LAIR1 antibody [NKTA255]

Name: Anti-LAIR1 PerCP/Cy5.5® antibody [NKTA255] (ab234263) IgG1 | Abcam
Brand: Abcam Limited
SKU: AB234263
Price: 525 USD
Availability: InStock

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(1 Publication)

Mouse Monoclonal LAIR1 antibody - conjugated to PerCP/Cy5.5®. Suitable for Flow Cyt and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Cell preparation containing LAIR1 protein.

View Alternative Names

CD305, LAIR1, Leukocyte-associated immunoglobulin-like receptor 1, LAIR-1, hLAIR1

1 Images

Flow Cytometry - PerCP/Cy5.5® Anti-LAIR1 antibody [NKTA255] (AB234263)

Flow Cyt

Supplier Data

Flow Cytometry - PerCP/Cy5.5® Anti-LAIR1 antibody [NKTA255] (AB234263)

Flow cytometric analysis of surface stained human peripheral blood cells, labeling LAIR1 with ab234263 at 4 μL per 10⁶ cells. Lymphocyte gate.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

NKTA255

Isotype

IgG1

Conjugation

PerCP/Cy5.5®

Excitation/Emission

Ex: 482nm, Em: 695nm

Carrier free

Reacts with

Human

Applications

Flow Cyt

applications

Immunogen

Cell preparation containing LAIR1 protein. The exact immunogen used to generate this antibody is proprietary information.

Q6GTX8

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "4 µL for 10^6 Cells", "FlowCyt-species-notes": "<p>(or 100 μL whole blood)</p>" } } }

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Product details

This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5, 268, 486 and related patents. Cy® and CyDye® are trademarks of Cytiva.

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Properties and storage information

Form

Liquid

Purification technique

Size-exclusion chromatography

Purification notes

ab234263 is conjugated with tandem dye PerCP-Cy™5.5 under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use.

Storage buffer

pH: 7.4 Preservative: 0.0975% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Store in the dark

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

LAIR1 also known as Leukocyte-Associated Immunoglobulin-like Receptor 1 is a transmembrane protein with a molecular weight of approximately 32 kDa. It expresses mainly on immune cells including T cells B cells and myeloid cells. This receptor by binding to collagen plays a significant role in controlling immune cell signaling. It acts as an inhibitory receptor which helps in maintaining immune system balance and prevents excessive immune responses.

Biological function summary

LAIR1 functions by modulating immune responses and maintaining homeostasis in the immune system. It is a part of a complex pathway involved in inhibitory signaling. When LAIR1 binds to its ligand such as collagen it transduces a signal that reduces activation of immune cells. This inhibition ensures that immune cells do not become overactive which could otherwise lead to tissue damage.

Pathways

LAIR1 participates in immune checkpoint regulation and collagen interaction pathways. It interacts with proteins such as ITIM-containing molecules which transmit inhibitory signals essential for regulating immune cell activation. It also indirectly connects to the TGF-beta signaling pathway which is vital for controlling cell differentiation and immune regulation.

LAIR1 is of interest due to its connection to autoimmune diseases and certain cancers. In autoimmune diseases such as rheumatoid arthritis dysregulated LAIR1 signaling may contribute to inflammation. The protein is also linked to cancer where its inhibitory signaling might affect tumor immune evasion. The connection between LAIR1 and these conditions involves proteins like autoimmune-related genes and tumor-associated surface molecules.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Functions as an inhibitory receptor that plays a constitutive negative regulatory role on cytolytic function of natural killer (NK) cells, B-cells and T-cells. Activation by Tyr phosphorylation results in recruitment and activation of the phosphatases PTPN6 and PTPN11. It also reduces the increase of intracellular calcium evoked by B-cell receptor ligation. May also play its inhibitory role independently of SH2-containing phosphatases. Modulates cytokine production in CD4+ T-cells, down-regulating IL2 and IFNG production while inducing secretion of transforming growth factor beta. Down-regulates also IgG and IgE production in B-cells as well as IL8, IL10 and TNF secretion. Inhibits proliferation and induces apoptosis in myeloid leukemia cell lines as well as prevents nuclear translocation of NF-kappa-B p65 subunit/RELA and phosphorylation of I-kappa-B alpha/CHUK in these cells. Inhibits the differentiation of peripheral blood precursors towards dendritic cells.

See full target information LAIR1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Bone & joint research 10:328-339 PubMed34024119

2021

Extracellular vesicles derived from mesenchymal stem cells containing microRNA-381 protect against spinal cord injury in a rat model via the BRD4/WNT5A axis.

Applications

Unspecified application

Species

Unspecified reactive species

Xufeng Jia,Guangping Huang,Shaohua Wang,Miao Long,Xiaojun Tang,Daxiong Feng,Qingzhong Zhou

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

PerCP/Cy5.5® Anti-LAIR1 antibody [NKTA255]

Flow Cytometry - PerCP/Cy5.5® Anti-LAIR1 antibody [NKTA255] (AB234263)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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