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PerCP/Cy5.5® Mouse IgG2b, kappa monoclonal [MPC-11] - Isotype Control Suitable for Flow Cyt and reacts with samples. Immunogen corresponding to Chemical / Small Molecule corresponding to KLH.

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Flow Cytometry - PerCP/Cy5.5® Mouse IgG2b, kappa monoclonal [MPC-11] - Isotype Control (AB239460), expandable thumbnail

Key facts

Isotype
IgG2b
Host species
Mouse
Conjugation
PerCP/Cy5.5®
Excitation/Emission
Ex: 482nm, Em: 695nm
Storage buffer

pH: 7.4
Preservative: 0.0975% Sodium azide
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • Chemical / Small Molecule corresponding to KLH.

Reactivity data

Application
Flow Cyt
Reactivity
Reacts
Dilution info
-
Notes

The reagent is intended as isotype control for flow cytometry analysis to establish the amount of non-specific antibody binding. For your particular experiment, use the same concentration of this isotype control antibody as the recommended working concentration of the antigen-specific antibody. Also, when working with prediluted antibodies, dilute the isotype control to the same concentration as is the concentration of the antigen-specific antibody in the prediluted antibody solution you are using. If under particular experimental conditions the background signal of the isotype control is too high (usually when working concentrations of used antibodies are above 10 μg per ml of incubation mixture), change the conditions of your experiment to reduce the background.

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PerCP/Cy5.5® Mouse IgG2b, kappa monoclonal [MPC-11] - Isotype Control Suitable for Flow Cyt and reacts with samples. Immunogen corresponding to Chemical / Small Molecule corresponding to KLH.

Key facts

Isotype
IgG2b
Conjugation
PerCP/Cy5.5®
Excitation/Emission
Ex: 482nm, Em: 695nm
Form
Liquid
Clonality
Monoclonal
Immunogen
  • Chemical / Small Molecule corresponding to KLH.
Clone number
MPC-11
Purification technique
Size-exclusion chromatography
Specificity

This mouse IgG2b (kappa) monoclonal antibody (clone MPC-11) reacts with an epitope irrelevant for a variety of resting, activated, live, and fixed human, mouse, and rat tissues.

Light chain type
kappa
Concentration
Loading...
Purification notes

Purified antibody is conjugated with PerCP/Cy5.5® under optimum conditions. The conjugate is purified by size-exclusion chromatography.

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Store in the dark

Notes

This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5, 268, 486 and related patents. Cy® and CyDye® are trademarks of Cytiva.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

1 product image

  • Flow Cytometry - PerCP/Cy5.5® Mouse IgG2b, kappa monoclonal [MPC-11] - Isotype Control (ab239460), expandable thumbnail

    Flow Cytometry - PerCP/Cy5.5® Mouse IgG2b, kappa monoclonal [MPC-11] - Isotype Control (ab239460)

    Flow cytometry surface nonspecific staining pattern of human peripheral whole blood stained using ab239460 at 15 µg/ml.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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