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AB218775

PerCP/Cy5.5® Anti-Thy1.2 antibody [30-H12]

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(2 Publications)

Rat Monoclonal CD90 / Thy1 antibody - conjugated to PerCP/Cy5.5®. Suitable for Flow Cyt and reacts with Mouse samples. Cited in 2 publications.

View Alternative Names

CD90, Thy-1, Thy1, Thy-1 membrane glycoprotein, Thy-1 antigen

1 Images
Flow Cytometry - PerCP/Cy5.5® Anti-Thy1.2 antibody [30-H12] (AB218775)
  • Flow Cyt

Supplier Data

Flow Cytometry - PerCP/Cy5.5® Anti-Thy1.2 antibody [30-H12] (AB218775)

Flow Cytometry analysis of C57Bl/6 splenocytes labeling Thy1.2 with ab218775 at 0.25 μg (solid line) or 0.25 μg PerCP-Cy5.5® Rat IgG2b isotype control (dashed line).

Key facts

Host species

Rat

Clonality

Monoclonal

Clone number

30-H12

Isotype

IgG2b

Conjugation

PerCP/Cy5.5®

Excitation/Emission

Ex: 482nm, Em: 695nm

Carrier free

No

Reacts with

Mouse

Applications

Flow Cyt

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p>Use 0.25μg per reaction.</p>" } } }
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Product details

Thy1.2 is expressed on thymocytes, mature T cells and neurons in mouse strains that express the CD90.2 allele (BALB/c, CBA, C3H, C57BL/6, SJL and others). ab218775 does not react with the CD90.1 allele expressed in mouse strains such as PL and AKR.

This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5, 268, 486 and related patents. Cy® and CyDye® are trademarks of Cytiva.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Purification notes
ab218775 was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.
Storage buffer
pH: 7.2 Preservative: 0.09% Sodium azide Constituents: 0.87% Sodium chloride, 0.12% Sodium dihydrogen phosphate, 0.1% Gelatin
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Thy1.2 also known as Thy-1 antigen or CD90 is a glycoprotein with a mass of approximately 25-35 kDa. It is expressed on the surface of many cell types including neurons thymocytes and fibroblasts. Thy1.2 is part of the immunoglobulin superfamily and plays a role in cell-cell interactions adhesion and signal transduction. Researchers often study Thy1.2 in the context of the mouse model as it is a well-defined target in murine immunology and neurobiology.
Biological function summary

The Thy1.2 protein influences cellular adhesion and migration processes. It does not form part of a known complex but regulates multiple interactions at the cellular membrane. In the nervous system Thy1.2 impacts neurite outgrowth and synapse formation. In the immune system it modulates T-cell activation and cytokine production. These actions make Thy1.2 integral to cellular communication and response mechanisms.

Pathways

Thy1.2 plays significant roles in signal transduction and cell adhesion pathways. It participates in the MAPK/ERK pathway which is important for cell growth and survival. Thy1.2 interacts closely with integrin proteins facilitating cell-matrix adhesion and signaling. This connection supports cellular processes that depend on environmental cues impacting both development and immune responses.

Thy1.2 shows significant associations with cancer and fibrosis. In cancer abnormal Thy1.2 expression or function can influence tumor growth and metastasis often in conjunction with other proteins such as integrins and CD44. In fibrosis Thy1.2 impacts fibroblast activity and extracellular matrix regulation leading to tissue remodeling. Studies explore how modulating Thy1.2 levels or function might offer therapeutic potential in these conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

May play a role in cell-cell or cell-ligand interactions during synaptogenesis and other events in the brain.
See full target information Thy1
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Advances in cancer biology - metastasis 7: PubMed37593105

2023

Benzophenone-3 exposure alters composition of tumor infiltrating immune cells and increases lung seeding of 4T1 breast cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Stephanie M Morin,Kelly J Gregory,Brenda Medeiros,Tigist Terefe,Reyhane Hoshyar,Ahmed Alhusseiny,Shiuan Chen,Richard C Schwartz,D Joseph Jerry,Laura N Vandenberg,Sallie S Schneider

Journal of ophthalmology & visual sciences 6: PubMed33829147

2021

BrdU Positive Cells Induced in a Genetic Mouse Model of Glaucoma.

Applications

Unspecified application

Species

Unspecified reactive species

J R Paris,N C Sklar,C L Linn
View all publications
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