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AB221256

PerCP Anti-Cytokeratin 19 antibody [EPR1579Y]

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(1 Publication)

Rabbit Recombinant Monoclonal Cytokeratin 19 antibody - conjugated to PerCP. Suitable for Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

View Alternative Names

Cytokeratin-19, Keratin-19, CK-19, K19, KRT19, ck19, ck 19

1 Images
Flow Cytometry (Intracellular) - PerCP Anti-Cytokeratin 19 antibody [EPR1579Y] (AB221256)
  • Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - PerCP Anti-Cytokeratin 19 antibody [EPR1579Y] (AB221256)

Overlay histogram showing MCF7 cells stained with ab221256 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab221256, 1/50 dilution) for 30 min at 22°C.

Isotype control antibody (black line) was rabbit IgG (monoclonal) PerCP (ab222107) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

Acquisition of >5,000 events were collected using a 50 mW Blue laser (488nm) and 685/35 bandpass filter.

This antibody gave a positive signal in MCF7 cells fixed with 4% formaldehyde (10 min)/permeabilized with 0.1% PBS-Triton X-100 for 15 min used under the same conditions.

  • Carrier free

    Anti-Cytokeratin 19 antibody [EPR1579Y] - BSA and Azide free

  • Unconjugated

    Anti-Cytokeratin 19 antibody [EPR1579Y]

  • 660 APC

    APC Anti-Cytokeratin 19 antibody [EPR1579Y]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-Cytokeratin 19 antibody [EPR1579Y]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-Cytokeratin 19 antibody [EPR1579Y]

  • 578 PE

    PE Anti-Cytokeratin 19 antibody [EPR1579Y]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR1579Y

Isotype

IgG

Conjugation

PerCP

Excitation/Emission

Ex: 490nm, Em: 675nm

Carrier free

No

Reacts with

Human, Human

Applications

Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Alternative Products

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Secondary Antibodies

AB150113

Goat Anti-Mouse IgG H&L (Alexa Fluor® 488)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/50", "FlowCytIntra-species-notes": "<p>The cellular localisation of this product has been verified in ICC/IF.<a href='/en-us/products/primary-antibodies/percp-rabbit-igg-monoclonal-epr25a-isotype-control-ab222107'>ab222107</a> - Rabbit monoclonal IgG (PerCP), is suitable for use as an isotype control with this antibody.</p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Do Not Freeze|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Cytokeratin 19 (CK19) also known as cytokera­tine 19 keratin 19 (KRT19) or CK19 is a type of intermediate filament found in epithelial cells. It serves as an essential component of the cytoskeleton. CK19 weighs approximately 40 kDa and is broadly expressed in simple epithelial tissues including liver intestine and various glandular epithelial cells. As a cytokeratin marker it often appears in combination with others like cytokeratin 17 in certain tissue types helping to maintain the structural integrity of cells.
Biological function summary

CK19 plays a role in maintaining the cytoskeletal framework that provides mechanical support and helps control cell size and shape. It is not part of a large protein complex but works closely with other cytokeratins to ensure the stability and resilience of epithelial tissues. The A53-B/A22.26 positive staining is often used to confirm its presence in clinical histopathology.

Pathways

CK19 connects to cellular processes especially involving the keratinization and epithelial cell differentiation pathways. It interacts with other cytokeratins including BA16 and KR17 to facilitate these processes. This interaction aids in modulating the dynamics of cytoskeletal rearrangement during cell movement division and differentiation.

CK19 overexpression is linked with certain cancers notably hepatocellular carcinoma and breast cancer. It can serve as a prognostic marker in these diseases often in conjunction with other markers like CK17. The involvement of CK19 in the epithelial-mesenchymal transition (EMT) process connects it to the progression and aggressiveness of tumors facilitated by interactions with proteins involved in EMT pathways.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Involved in the organization of myofibers. Together with KRT8, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.
See full target information KRT19
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Aging 16:12498-12509 PubMed38747738

2024

SHP2 mediates STAT3/STAT6 signaling pathway in TAM to inhibit proliferation and metastasis of lung adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Guojing Chai,Yingbo Nan,Haili Zhao,Qingchuan Hu
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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