PerCP Rabbit IgG, monoclonal [EPR25A] - Isotype Control

2 product images

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  Flow Cytometry (Intracellular) - PerCP Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab222107)

  Flow cytometry overlay histogram showing PC12 (NGF differentiated) cells stained with PerCP Anti-CD90 / Thy1 antibody [MRC OX-7] ab216354 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were incubated in 1x PBS containing 10% normal goat serum to block non-specific protein-protein interaction, followed by the antibody (PerCP Anti-CD90 / Thy1 antibody [MRC OX-7] ab216354) (1x 106 in 100μl at 5μg/ml (1/100)) for 30 min at 22°C. Isotype control antibody (black line) was Rabbit IgG (monoclonal) PerCP (ab222107) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control. Acquisition of >5000 events were collected using a 50mW Blue laser (488nm) and 690/50 bandpass filter.

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  Flow Cytometry (Intracellular) - PerCP Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab222107)

  Overlay histogram showing NIH3T3 cells stained with ab220526 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab220526, 1/50 dilution) for 30 min at 22°C.
  

  Isotype control antibody (black line) was rabbit IgG (monoclonal) PerCP (ab222107) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

  Acquisition of >5,000 events were collected using a 50 mW Blue laser (488nm) and 685/35 bandpass filter.

  This antibody gave a positive signal in NIH3T3 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Triton X-100 for 15 min used under the same conditions.