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AB222107

PerCP Rabbit IgG, monoclonal [EPR25A] - Isotype Control

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PerCP Rabbit IgG, monoclonal [EPR25A] - Isotype Control Suitable for Flow Cyt (Intra) and reacts with samples.
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Flow Cytometry (Intracellular) - PerCP Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB222107)
  • Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - PerCP Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB222107)

Overlay histogram showing NIH3T3 cells stained with ab220526 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab220526, 1/50 dilution) for 30 min at 22°C.

Isotype control antibody (black line) was rabbit IgG (monoclonal) PerCP (ab222107) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

Acquisition of >5,000 events were collected using a 50 mW Blue laser (488nm) and 685/35 bandpass filter.

This antibody gave a positive signal in NIH3T3 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Triton X-100 for 15 min used under the same conditions.

Flow Cytometry (Intracellular) - PerCP Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB222107)
  • Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - PerCP Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB222107)

Flow cytometry overlay histogram showing PC12 (NGF differentiated) cells stained with ab216354 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were incubated in 1x PBS containing 10% normal goat serum to block non-specific protein-protein interaction, followed by the antibody (ab216354) (1x 106 in 100μl at 5μg/ml (1/100)) for 30 min at 22°C. Isotype control antibody (black line) was Rabbit IgG (monoclonal) PerCP (ab222107) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control. Acquisition of >5000 events were collected using a 50mW Blue laser (488nm) and 690/50 bandpass filter.

  • Unconjugated

    Rabbit IgG, monoclonal [EPR25A] - Isotype Control

  • Biotin

    Biotin Isotype Control [EPR25A]

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Rabbit IgG, monoclonal [EPR25A] - Isotype Control

  • 578 PE

    PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control

  • 603 Alexa Fluor® 568

    Alexa Fluor® 568 Rabbit IgG, monoclonal [EPR25A] - Isotype Control

  • 519 FITC

    FITC Rabbit IgG, monoclonal [EPR25A] - Isotype Control

  • Carrier free

    Rabbit IgG, monoclonal [EPR25A] - Isotype Control - BSA and Azide Free

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control

  • HRP

    HRP Rabbit IgG, monoclonal [EPR25A] - Isotype Control

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Rabbit IgG, monoclonal [EPR25A] - Isotype Control

  • 421 Alexa Fluor® 405

    Alexa Fluor® 405 Rabbit IgG, monoclonal [EPR25A] - Isotype Control

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Rabbit IgG, monoclonal [EPR25A] - Isotype Control

  • 660 APC

    APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR25A

Isotype

IgG

Conjugation

PerCP

Excitation/Emission

Ex: 490nm, Em: 675nm

Carrier free

No

Applications

Flow Cyt (Intra)

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p><strong>Please note: </strong>This product should be diluted to the same concentration (not dilution) of the primary antibody to be used.</p>" } } }
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Product details

KLH is often used in molecular immunology as a carrier protein conjugated to low molecular weight molecules such as peptides, amino acids, nucleic acids, drugs or toxins to render them more immunogenic due to the size of the conjugate complex and the immunogenicity of KLH.

Immunogen
Chemical / Small Molecule conjugated to keyhole limpet haemocyanin. KLH is a copper containing oxygen carrier occurring freely dissolved in the hemolymph of many molluscs and arthropods. KLH forms a large complex composed of ~50 kDa subunits.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Do Not Freeze|Store in the dark
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com