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Rabbit Recombinant Monoclonal Peregrin/BRPF1 antibody. Suitable for ICC/IF, WB, Flow Cyt (Intra) and reacts with Mouse, Human, Rat samples. Cited in 1 publication.

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Images

Western blot - Anti-Peregrin/BRPF1 antibody [EPR24069-57] (AB259840), expandable thumbnail
  • Western blot - Anti-Peregrin/BRPF1 antibody [EPR24069-57] (AB259840), expandable thumbnail
  • Western blot - Anti-Peregrin/BRPF1 antibody [EPR24069-57] (AB259840), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Peregrin/BRPF1 antibody [EPR24069-57] (AB259840), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Peregrin/BRPF1 antibody [EPR24069-57] (AB259840), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFWBIHC-PFlow Cyt (Intra)
Human
Tested
Tested
Not recommended
Tested
Mouse
Tested
Tested
Not recommended
Tested
Rat
Expected
Tested
Not recommended
Expected

Tested
Tested

Species
Mouse
Dilution info
1/50
Notes

-

Species
Human
Dilution info
1/50
Notes

-

Expected
Expected

Species
Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/1000
Notes

-

Species
Rat
Dilution info
1/1000
Notes

-

Species
Human
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/50
Notes

-

Species
Human
Dilution info
1/50
Notes

-

Expected
Expected

Species
Rat
Dilution info
1/50
Notes

-

Target data

Function

Scaffold subunit of various histone acetyltransferase (HAT) complexes, such as the MOZ/MORF and HBO1 complexes, which have a histone H3 acetyltransferase activity (PubMed:16387653, PubMed:24065767, PubMed:27939640). Plays a key role in HBO1 complex by directing KAT7/HBO1 specificity towards histone H3 'Lys-14' acetylation (H3K14ac) (PubMed:24065767). Some HAT complexes preferentially mediate histone H3 'Lys-23' (H3K23ac) acetylation (PubMed:27939640). Positively regulates the transcription of RUNX1 and RUNX2 (PubMed:18794358).

Alternative names

BR140, BRPF1, Peregrin, Bromodomain and PHD finger-containing protein 1, Protein Br140

Recommended products

Rabbit Recombinant Monoclonal Peregrin/BRPF1 antibody. Suitable for ICC/IF, WB, Flow Cyt (Intra) and reacts with Mouse, Human, Rat samples. Cited in 1 publication.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR24069-57
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Peregrin/BRPF1 also known as Peregrins is a protein with a molecular mass around 130 kDa. It functions primarily as a component of the MOZ/MORF histone acetyltransferase (HAT) complex. Peregrin/BRPF1 plays a mechanical role in the regulation of gene expression by altering chromatin structure through histone modification. It is expressed in various tissues with higher expression in the brain and testis indicating its importance in neurological and reproductive functions.

Biological function summary

Peregrin/BRPF1 is a part of the MOZ/MORF complex which is important for acetylating histone H3 at lysine 23 influencing transcriptional activation. This action aids in transcription regulation and chromatin remodeling facilitating access of transcription factors to DNA. By participating in these processes Peregrin/BRPF1 helps maintain proper gene expression patterns essential for normal cell function and development.

Pathways

BRPF1 has roles in processes like the cell cycle and embryonic development pathways because its histone acetylation influences gene expression. It interacts with other acetyltransferases such as EP300 and CREBBP which play key roles in transcription regulation and chromatin remodeling within these pathways. These interactions highlight BRPF1's critical involvement in coordinating cellular events and developmental processes.

Associated diseases and disorders

Dysregulation of BRPF1 is associated with neurological disorders such as intellectual disability and specific cancers due to its role in chromatin remodeling and gene expression. In the context of intellectual disability it interacts with other proteins involved in chromatin structure like NSD1 and KDM6A. These connections illustrate the significant impact of Peregrin/BRPF1's regulation and its influence on health when its function becomes impaired.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

7 product images

  • Western blot - Anti-Peregrin/BRPF1 antibody [EPR24069-57] (ab259840), expandable thumbnail

    Western blot - Anti-Peregrin/BRPF1 antibody [EPR24069-57] (ab259840)

    False colour image of Western blot: Anti-Peregrin/BRPF1 antibody [EPR24069-57] staining at 1/500 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab259840 was shown to bind specifically to Peregrin/BRPF1. A band was observed at 120 kDa in wild-type HEK-293T cell lysates with no signal observed at this size in BRPF1 knockout cell line Human BRPF1 (Peregrin) knockout HEK-293T cell line ab266549 (knockout cell lysate Human BRPF1 (Peregrin) knockout HEK-293T cell lysate ab258795). To generate this image, wild-type and BRPF1 knockout HEK-293T cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) at 1/20000 dilution.

    Lanes 1, 2, 3, 5 and 6: Western blot - Anti-Peregrin/BRPF1 antibody [EPR24069-57] (ab259840) at 1/500 dilution

    Lane 4: Western blot - Anti-Peregrin/BRPF1 antibody [EPR24069-57] (ab259840)

    Lane 1: Wild-type HEK-293T cell lysate at 20 µg

    Lanes 2 - 3: BRPF1 knockout HEK-293T cell lysate at 20 µg

    Lane 2: Western blot - Human BRPF1 (Peregrin) knockout HEK-293T cell line (Human BRPF1 (Peregrin) knockout HEK-293T cell line ab266549)

    Lane 4: Empty

    Lane 5: PC-3 cell lysate at 20 µg

    Lane 6: HepG2 cell lysate at 20 µg

    Performed under reducing conditions.

    Predicted band size: 137 kDa

    Observed band size: 120 kDa

  • Western blot - Anti-Peregrin/BRPF1 antibody [EPR24069-57] (ab259840), expandable thumbnail

    Western blot - Anti-Peregrin/BRPF1 antibody [EPR24069-57] (ab259840)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    The molecular weight observed is consistent with what has been described in the literature (PMID: 7906940).

    Lysates were made freshly and used in WB immediately to minimize protein degradation.

    Exposure time: 3 minutes

    All lanes: Western blot - Anti-Peregrin/BRPF1 antibody [EPR24069-57] (ab259840) at 1/1000 dilution

    Lane 1: HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 2: 293T (human embryonic kidney epithelial cell) whole cell lysate at 20 µg

    Lane 3: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg

    Lane 4: PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 137 kDa

    Observed band size: 150-160 kDa

  • Western blot - Anti-Peregrin/BRPF1 antibody [EPR24069-57] (ab259840), expandable thumbnail

    Western blot - Anti-Peregrin/BRPF1 antibody [EPR24069-57] (ab259840)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    Exposure time: 8 seconds

    All lanes: Western blot - Anti-Peregrin/BRPF1 antibody [EPR24069-57] (ab259840) at 1/5000 dilution

    Lane 1: His-tagged human BRPF1 recombinant protein at 0.01 µg

    Lane 2: His-tagged human BRPF3 recombinant protein at 0.2 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 137 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-Peregrin/BRPF1 antibody [EPR24069-57] (ab259840), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Peregrin/BRPF1 antibody [EPR24069-57] (ab259840)

    Immunofluorescent analysis of 100% methanol-fixed, 0.1% TritonX-100 permeabilized HeLa cells labelling Peregrin/BRPF1 with ab259840 at 1/50 (11.34 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green). Confocal image showing nuclear staining in HeLa cells is observed.

    Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-Peregrin/BRPF1 antibody [EPR24069-57] (ab259840), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Peregrin/BRPF1 antibody [EPR24069-57] (ab259840)

    Immunofluorescent analysis of 100% methanol-fixed, 0.1% TritonX-100 permeabilized NIH/3T3 cells labelling Peregrin/BRPF1 with ab259840 at 1/50 (11.34 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green). Confocal image showing nuclear staining in NIH/3T3 cells is observed.

    Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution.

  • Flow Cytometry (Intracellular) - Anti-Peregrin/BRPF1 antibody [EPR24069-57] (ab259840), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-Peregrin/BRPF1 antibody [EPR24069-57] (ab259840)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HeLa (Human cervix adenocarcinoma cell) cells labelling Peregrin/BRPF1 with ab259840 at 1/500 dilution (0.1μg) (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as the secondary antibody.

  • Flow Cytometry (Intracellular) - Anti-Peregrin/BRPF1 antibody [EPR24069-57] (ab259840), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-Peregrin/BRPF1 antibody [EPR24069-57] (ab259840)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized 4T1 (mouse mammary gland carcinoma epithelial cell) cells labelling Peregrin/BRPF1 with ab259840 at 1/500 dilution (0.1μg) (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as the secondary antibody.

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