AB47225

Anti-Perforin antibody [B-D48] - BSA and Azide free

Name: Anti-Perforin antibody [B-D48] - BSA and Azide free (ab47225) | Abcam
Brand: Abcam Limited
SKU: AB47225
Rating: 4 (1 reviews)

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What is this?

(1 Review)

(21 Publications)

Mouse Monoclonal Perforin antibody. Carrier free. Suitable for IHC-P and reacts with Human samples. Cited in 21 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human PRF1.

View Alternative Names

PFP, PRF1, Perforin-1, P1, Cytolysin, Lymphocyte pore-forming protein

1 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Perforin antibody [B-D48] - BSA and Azide free (AB47225)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Perforin antibody [B-D48] - BSA and Azide free (AB47225)

IHC image of Perforin staining in human spleen formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab47225, 10μg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

519 FITC

FITC Anti-Perforin antibody [B-D48]
578 PE

PE Anti-Perforin antibody [B-D48]

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

B-D48

Isotype

IgG1

Light chain type

kappa

Carrier free

Yes

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Recombinant Full Length Protein corresponding to Human PRF1.

P14222

Specificity

<p>Recognizes human Perforin, a 70 kDa protein.</p>

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Ion exchange chromatography

Storage buffer

pH: 7.3 - 7.5 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C|-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Perforin also referred to as PRF1 is a pore-forming protein that plays an important role in immune response. It appears as a 67-kDa protein and is mainly expressed in cytotoxic T cells and natural killer (NK) cells. The function of perforin involves the formation of transmembrane pores in the membranes of target cells aiding in the delivery of pro-apoptotic molecules. This mechanism is essential for the destruction of virus-infected cells and tumor cells. Techniques like perforin IHC and perforin ELISA are suitable for detecting and quantifying this protein in various samples.

Biological function summary

Perforin helps the immune system respond effectively against infected or malignant cells. It works in conjunction with granzymes forming a complex that perforates target cell membranes allowing granzymes to enter and induce apoptosis. Perforin's role in immune surveillance prevents the propagation of harmful cells within the body. Deficiency or malfunction in perforin can lead to compromised immune function or immune cell dysregulation.

Pathways

Perforin integrates into the cytotoxic T lymphocyte (CTL) and NK cell-mediated cytotoxicity pathways. These pathways include critical interactions with proteins like granzymes and Fas ligand (FasL) which orchestrate the apoptosis of abnormal cells. Through these pathways perforin ensures the body's ability to identify and eliminate threats. The granule exocytosis pathway in particular heavily depends on perforin's activity to execute effective immune responses.

Mutations or defects in perforin are linked to familial hemophagocytic lymphohistiocytosis (FHL) and some types of cancer. These conditions arise when perforin's ability to function correctly is impaired. For instance in FHL defective perforin hampers the immune cells' ability to kill target cells leading to uncontrolled immune activation. Furthermore in the context of cancer the proper functioning of perforin alongside proteins like granzyme B is critical for the surveillance and elimination of tumor cells.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Pore-forming protein that plays a key role in granzyme-mediated programmed cell death, and in defense against virus-infected or neoplastic cells (PubMed : 20889983, PubMed : 21037563, PubMed : 24558045, PubMed : 9058810, PubMed : 9164947). Plays an important role in killing other cells that are recognized as non-self by the immune system, e.g. in transplant rejection or some forms of autoimmune disease (PubMed : 9058810). Can insert into the membrane of target cells in its calcium-bound form, oligomerize and form large pores (PubMed : 20889983, PubMed : 21037563). Promotes cytolysis and apoptosis of target cells by mediating the passage and uptake of cytotoxic granzymes (PubMed : 20038786, PubMed : 20225066, PubMed : 24558045, PubMed : 32299851). Facilitates the delivery of cationic cargo protein, while anionic or neural proteins are not delivered efficiently (PubMed : 24558045). Perforin pores allow the release of mature caspase-7 (CASP7) into the extracellular milieu (By similarity).

See full target information PRF1

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Publications (21)

Recent publications for all applications. Explore the full list and refine your search

Nature 646:462-473 PubMed40836096

2025

Cancer-induced nerve injury promotes resistance to anti-PD-1 therapy.

Applications

Unspecified application

Species

Unspecified reactive species

Erez N Baruch,Frederico O Gleber-Netto,Priyadharsini Nagarajan,Xiayu Rao,Shamima Akhter,Tuany Eichwald,Tongxin Xie,Mohammad Balood,Adebayo Adewale,Shorook Naara,Hinduja N Sathishkumar,Shajedul Islam,William McCarthy,Brandi J Mattson,Renata Ferrarotto,Michael K Wong,Michael A Davies,Sonali Jindal,Sreyashi Basu,Karine Roversi,Amin Reza Nikpoor,Maryam Ahmadi,Ali Ahmadi,Catherine Harwood,Irene Leigh,Dennis Gong,Paulino Tallón de Lara,Derrick L Tao,Tara M Davidson,Nadim J Ajami,Andrew Futreal,Kunal Rai,Veena Kochat,Micah Castillo,Preethi Gunaratne,Ryan P Goepfert,Sharia D Hernandez,Nikhil I Khushalani,Jing Wang,Stephanie S Watowich,George A Calin,Michael R Migden,Mona Yuan,Naijiang Liu,Yi Ye,William L Hwang,Paola D Vermeer,Nisha J D'Silva,Yuri L Bunimovich,Dan Yaniv,Jared K Burks,Javier Gomez,Patrick M Dougherty,Kenneth Y Tsai,James P Allison,Padmanee Sharma,Jennifer A Wargo,Jeffrey N Myers,Sebastien Talbot,Neil D Gross,Moran Amit

World journal of surgical oncology 22:128 PubMed38725005

2024

METTL14 regulates CD8T-cell activation and immune responses to anti-PD-1 therapy in lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Chongqi Sun,Jian Wang,Huixing Li,Luyao Liu,Yu Lin,Ling Zhang,Xianglong Zu,Yizhi Zhu,Yongqian Shu,Dong Shen,Qiong Wang,Yiqian Liu

Nature immunology 24:2135-2149 PubMed37932456

2023

Harnessing CD3 diversity to optimize CAR T cells.

Applications

Unspecified application

Species

Unspecified reactive species

Rubí M-H Velasco Cárdenas,Simon M Brandl,Ana Valeria Meléndez,Alexandra Emilia Schlaak,Annabelle Buschky,Timo Peters,Fabian Beier,Bryan Serrels,Sanaz Taromi,Katrin Raute,Simon Hauri,Matthias Gstaiger,Silke Lassmann,Johannes B Huppa,Melanie Boerries,Geoffroy Andrieux,Bertram Bengsch,Wolfgang W Schamel,Susana Minguet

Cell 186:4632-4651.e23 PubMed37776858

2023

Multi-omics analysis of mucosal and systemic immunity to SARS-CoV-2 after birth.

Applications

Unspecified application

Species

Unspecified reactive species

Florian Wimmers,Allison R Burrell,Yupeng Feng,Hong Zheng,Prabhu S Arunachalam,Mengyun Hu,Sara Spranger,Lindsay E Nyhoff,Devyani Joshi,Meera Trisal,Mayanka Awasthi,Lorenza Bellusci,Usama Ashraf,Sangeeta Kowli,Katherine C Konvinse,Emily Yang,Michael Blanco,Kathryn Pellegrini,Gregory Tharp,Thomas Hagan,R Sharon Chinthrajah,Tran T Nguyen,Alba Grifoni,Alessandro Sette,Kari C Nadeau,David B Haslam,Steven E Bosinger,Jens Wrammert,Holden T Maecker,Paul J Utz,Taia T Wang,Surender Khurana,Purvesh Khatri,Mary A Staat,Bali Pulendran

eLife 12: PubMed36688525

2023

Compartmentalization and persistence of dominant (regulatory) T cell clones indicates antigen skewing in juvenile idiopathic arthritis.

Applications

Unspecified application

Species

Unspecified reactive species

Gerdien Mijnheer,Nila Hendrika Servaas,Jing Yao Leong,Arjan Boltjes,Eric Spierings,Phyllis Chen,Liyun Lai,Alessandra Petrelli,Sebastiaan Vastert,Rob J de Boer,Salvatore Albani,Aridaman Pandit,Femke van Wijk

Cancer discovery 13:114-131 PubMed36259971

2022

Intraventricular B7-H3 CAR T Cells for Diffuse Intrinsic Pontine Glioma: Preliminary First-in-Human Bioactivity and Safety.

Applications

Unspecified application

Species

Unspecified reactive species

Nicholas A Vitanza,Ashley L Wilson,Wenjun Huang,Kristy Seidel,Christopher Brown,Joshua A Gustafson,Jason K Yokoyama,Adam J Johnson,Blake A Baxter,Ryan W Koning,Aquene N Reid,Michael Meechan,Matthew C Biery,Carrie Myers,Stephanie D Rawlings-Rhea,Catherine M Albert,Samuel R Browd,Jason S Hauptman,Amy Lee,Jeffrey G Ojemann,Michael E Berens,Matthew D Dun,Jessica B Foster,Erin E Crotty,Sarah E S Leary,Bonnie L Cole,Francisco A Perez,Jason N Wright,Rimas J Orentas,Tony Chour,Evan W Newell,Jeffrey R Whiteaker,Lei Zhao,Amanda G Paulovich,Navin Pinto,Juliane Gust,Rebecca A Gardner,Michael C Jensen,Julie R Park

Nature 596:410-416 PubMed34252919

2021

Systems vaccinology of the BNT162b2 mRNA vaccine in humans.

Applications

Unspecified application

Species

Unspecified reactive species

Prabhu S Arunachalam,Madeleine K D Scott,Thomas Hagan,Chunfeng Li,Yupeng Feng,Florian Wimmers,Lilit Grigoryan,Meera Trisal,Venkata Viswanadh Edara,Lilin Lai,Sarah Esther Chang,Allan Feng,Shaurya Dhingra,Mihir Shah,Alexandra S Lee,Sharon Chinthrajah,Sayantani B Sindher,Vamsee Mallajosyula,Fei Gao,Natalia Sigal,Sangeeta Kowli,Sheena Gupta,Kathryn Pellegrini,Gregory Tharp,Sofia Maysel-Auslender,Sydney Hamilton,Hadj Aoued,Kevin Hrusovsky,Mark Roskey,Steven E Bosinger,Holden T Maecker,Scott D Boyd,Mark M Davis,Paul J Utz,Mehul S Suthar,Purvesh Khatri,Kari C Nadeau,Bali Pulendran

Immunity 54:1594-1610.e11 PubMed34174183

2021

Deep spatial profiling of human COVID-19 brains reveals neuroinflammation with distinct microanatomical microglia-T-cell interactions.

Applications

Unspecified application

Species

Unspecified reactive species

Marius Schwabenland,Henrike Salié,Jovan Tanevski,Saskia Killmer,Marilyn Salvat Lago,Alexandra Emilia Schlaak,Lena Mayer,Jakob Matschke,Klaus Püschel,Antonia Fitzek,Benjamin Ondruschka,Henrik E Mei,Tobias Boettler,Christoph Neumann-Haefelin,Maike Hofmann,Angele Breithaupt,Nafiye Genc,Christine Stadelmann,Julio Saez-Rodriguez,Peter Bronsert,Klaus-Peter Knobeloch,Thomas Blank,Robert Thimme,Markus Glatzel,Marco Prinz,Bertram Bengsch

Journal of experimental & clinical cancer research 39:238 PubMed33168024

2020

MiRNA-340-5p mediates the functional and infiltrative promotion of tumor-infiltrating CD8 T lymphocytes in human diffuse large B cell lymphoma.

Applications

Unspecified application

Species

Unspecified reactive species

Yangyang Xu,Zhenchuan Liu,Lixin Lv,Ping Li,Bing Xiu,Wenbin Qian,Aibin Liang

Cell reports. Medicine 1:100039 PubMed33205061

2020

Human Tumor-Infiltrating MAIT Cells Display Hallmarks of Bacterial Antigen Recognition in Colorectal Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Shamin Li,Yannick Simoni,Etienne Becht,Chiew Yee Loh,Naisi Li,Daniel Lachance,Si-Lin Koo,Teck Por Lim,Emile Kwong Wei Tan,Ronnie Mathew,Andrew Nguyen,Justin Golovato,Julia D Berkson,Martin Prlic,Bernett Lee,Samuel S Minot,Niranjan Nagarajan,Neelendu Dey,Daniel S W Tan,Iain B Tan,Evan W Newell

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Perforin antibody [B-D48] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Perforin antibody [B-D48] - BSA and Azide free (AB47225)

Related conjugates and formulations (2)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Immunogen

Specificity

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (21)

Product promise