Rabbit Polyclonal TACY antibody. Suitable for WB and reacts with Recombinant full length protein - Clostridium perfringens samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Clostridium perfringens str. 13 pfo.
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Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.03% Proclin 300
Constituents: PBS, 50% Glycerol (glycerin, glycerine)- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Recombinant Full Length Protein corresponding to Clostridium perfringens str. 13 pfo. Database link P0C2E9
Reactivity data
Select an application| WB | |
|---|---|
| Clostridium perfringens | Predicted |
| Recombinant full length protein - Clostridium perfringens | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Clostridium perfringens | Dilution info 1/500 - 1/5000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Clostridium perfringens | Dilution info - | Notes - |
Target data
Function
A cholesterol-dependent toxin that causes cytolysis by forming pores in cholesterol-containing host membranes. After binding to target membranes, the protein assembles into a pre-pore complex. A major conformational change leads to insertion in the host membrane and formation of an oligomeric pore complex. Cholesterol is required for binding to host cell membranes, membrane insertion and pore formation; cholesterol binding is mediated by a Thr-Leu pair in the C-terminus. Can be reversibly inactivated by oxidation.
Alternative names
Perfringolysin O, PFO, Theta-toxin, Thiol-activated cytolysin, CPE0163, pfoR, pfoA, pfo
Recommended products
Rabbit Polyclonal TACY antibody. Suitable for WB and reacts with Recombinant full length protein - Clostridium perfringens samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Clostridium perfringens str. 13 pfo.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.03% Proclin 300
Constituents: PBS, 50% Glycerol (glycerin, glycerine)- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Recombinant Full Length Protein corresponding to Clostridium perfringens str. 13 pfo. Database link P0C2E9
- Purification technique
Affinity purification Protein G
- Concentration
- Purification notes
Purity >95%.
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Perfringolysin O also known as Theta-toxin is a toxin produced by the bacterium Clostridium perfringens. It is a cholesterol-dependent cytolysin with a molecular mass of approximately 54 kDa. The toxin is expressed by bacteria and is secreted into the extracellular environment. Mechanically Perfringolysin O binds to cholesterol in eukaryotic cell membranes forming pores that lead to cell lysis. This toxin exemplifies the ability of pathogens to compromise host cell integrity.
- Biological function summary
The protein plays a role in bacterial pathogenesis by disrupting host cell membranes. Perfringolysin O targets cholesterol-rich domains within the plasma membrane leading to increased permeability and cell death. Although it does not form a complex with other proteins for its activity its function significantly impacts cellular systems by inducing necrosis and potentially triggering immune responses as host cells attempt to mitigate its effects.
- Pathways
Perfringolysin O participates in the infection pathways of Clostridium perfringens-related diseases. It is involved in pathways that disrupt membrane integrity contributing to cellular damage and inflammatory responses. The toxin operates alongside other bacterial proteins such as alpha-toxin to facilitate the progression of infections. These proteins synergistically interact within their respective pathways to enhance the bacterium's virulence.
- Associated diseases and disorders
Perfringolysin O is strongly associated with gas gangrene and food poisoning. These conditions arise due to Clostridium perfringens infection where the toxin plays a pivotal role in tissue degradation and systemic toxicity. Through its action Perfringolysin O contributes to the rapid progression of gas gangrene when it acts in concert with alpha-toxin both of which are critical factors in the severity of the disease. Understanding these interactions aids in devising therapeutic strategies against these infections.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
Western blot - Anti-Perfringolysin O antibody (ab225685)
All lanes: Western blot - Anti-Perfringolysin O antibody (ab225685) at 1/500 dilution
Lane 1: Recombinant Clostridium perfringens Perfringolysin O protein at 0.08 µg
Lane 2: Recombinant Clostridium perfringens Perfringolysin O protein at 0.04 µg
Lane 3: Recombinant Clostridium perfringens Perfringolysin O protein at 0.02 µg
Lane 4: Recombinant Clostridium perfringens Perfringolysin O protein at 0.01 µg
SecondaryAll lanes: Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 56 kDa
Observed band size: 56 kDa
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