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AB225685

Anti-Perfringolysin O antibody

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(2 Publications)

Rabbit Polyclonal TACY antibody. Suitable for WB and reacts with Recombinant full length protein - Clostridium perfringens samples. Cited in 2 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Clostridium perfringens str. 13 pfo.

View Alternative Names

pfoA, pfoR, CPE0163, pfo, Perfringolysin O, PFO, Theta-toxin, Thiol-activated cytolysin

1 Images
Western blot - Anti-Perfringolysin O antibody (AB225685)
  • WB

Supplier Data

Western blot - Anti-Perfringolysin O antibody (AB225685)

All lanes:

Western blot - Anti-Perfringolysin O antibody (ab225685) at 1/500 dilution

Lane 1:

Recombinant Clostridium perfringens Perfringolysin O protein at 0.08 µg

Lane 2:

Recombinant Clostridium perfringens Perfringolysin O protein at 0.04 µg

Lane 3:

Recombinant Clostridium perfringens Perfringolysin O protein at 0.02 µg

Lane 4:

Recombinant Clostridium perfringens Perfringolysin O protein at 0.01 µg

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/50000 dilution

Predicted band size: 56 kDa

Observed band size: 56 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Applications

WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Clostridium perfringens str. 13 pfo.

P0C2E9

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Clostridium perfringens": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Recombinant full length protein - Clostridium perfringens": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purity >95%.
Storage buffer
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Perfringolysin O also known as Theta-toxin is a toxin produced by the bacterium Clostridium perfringens. It is a cholesterol-dependent cytolysin with a molecular mass of approximately 54 kDa. The toxin is expressed by bacteria and is secreted into the extracellular environment. Mechanically Perfringolysin O binds to cholesterol in eukaryotic cell membranes forming pores that lead to cell lysis. This toxin exemplifies the ability of pathogens to compromise host cell integrity.
Biological function summary

The protein plays a role in bacterial pathogenesis by disrupting host cell membranes. Perfringolysin O targets cholesterol-rich domains within the plasma membrane leading to increased permeability and cell death. Although it does not form a complex with other proteins for its activity its function significantly impacts cellular systems by inducing necrosis and potentially triggering immune responses as host cells attempt to mitigate its effects.

Pathways

Perfringolysin O participates in the infection pathways of Clostridium perfringens-related diseases. It is involved in pathways that disrupt membrane integrity contributing to cellular damage and inflammatory responses. The toxin operates alongside other bacterial proteins such as alpha-toxin to facilitate the progression of infections. These proteins synergistically interact within their respective pathways to enhance the bacterium's virulence.

Perfringolysin O is strongly associated with gas gangrene and food poisoning. These conditions arise due to Clostridium perfringens infection where the toxin plays a pivotal role in tissue degradation and systemic toxicity. Through its action Perfringolysin O contributes to the rapid progression of gas gangrene when it acts in concert with alpha-toxin both of which are critical factors in the severity of the disease. Understanding these interactions aids in devising therapeutic strategies against these infections.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

A cholesterol-dependent toxin that causes cytolysis by forming pores in cholesterol-containing host membranes. After binding to target membranes, the protein assembles into a pre-pore complex. A major conformational change leads to insertion in the host membrane and formation of an oligomeric pore complex. Cholesterol is required for binding to host cell membranes, membrane insertion and pore formation; cholesterol binding is mediated by a Thr-Leu pair in the C-terminus. Can be reversibly inactivated by oxidation.
See full target information pfo
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Molecular metabolism 91:102077 PubMed39617267

2024

Mammalian mitochondrial inorganic polyphosphate (polyP) and cell signaling: Crosstalk between polyP and the activity of AMPK.

Applications

Unspecified application

Species

Unspecified reactive species

Renata T Da Costa,Anna Nichenko,Matheus M Perez,Malgorzata Tokarska-Schlattner,Sheida Kavehmoghaddam,Vedangi Hambardikar,Ernest R Scoma,Erin L Seifert,Uwe Schlattner,Joshua C Drake,Maria E Solesio

Cell reports 42:112046 PubMed36708514

2023

Network analysis of large-scale ImmGen and Tabula Muris datasets highlights metabolic diversity of tissue mononuclear phagocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Anastasiia Gainullina,Denis A Mogilenko,Li-Hao Huang,Helena Todorov,Vipin Narang,Ki-Wook Kim,Lim Sheau Yng,Andrew Kent,Baosen Jia,Kumba Seddu,Karen Krchma,Jun Wu,Karine Crozat,Elena Tomasello,Regine Dress,Peter See,Charlotte Scott,Sophie Gibbings,Geetika Bajpai,Jigar V Desai,Barbara Maier,Sébastien This,Peter Wang,Stephanie Vargas Aguilar,Lucie Poupel,Sébastien Dussaud,Tyng-An Zhou,Veronique Angeli,J Magarian Blander,Kyunghee Choi,Marc Dalod,Ivan Dzhagalov,Emmanuel L Gautier,Claudia Jakubzick,Kory Lavine,Michail S Lionakis,Helena Paidassi,Michael H Sieweke,Florent Ginhoux,Martin Guilliams,Christophe Benoist,Miriam Merad,Gwendalyn J Randolph,Alexey Sergushichev,Maxim N Artyomov
View all publications
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