AB176801

Anti-Pericentrin 1/FROUNT antibody

Name: Anti-Pericentrin 1/FROUNT antibody (ab176801) | Abcam
Brand: Abcam Limited
SKU: AB176801

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(1 Publication)

Rabbit Polyclonal Pericentrin 1/FROUNT antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human NUP85 aa 1-50.

View Alternative Names

NUP75, PCNT1, NUP85, Nuclear pore complex protein Nup85, 85 kDa nucleoporin, FROUNT, Nucleoporin Nup75, Nucleoporin Nup85, Pericentrin-1

2 Images

Supplier Data

Immunoprecipitation - Anti-Pericentrin 1/FROUNT antibody (AB176801)

Detection of Pericentrin 1 in Immunoprecipitates of 293T whole cell lysate (1 mg for IP, 20% of IP loaded) using ab176801 at 6 μg/mg lysate for IP (Lane 1) and at 1 μg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP.

Detection : Chemiluminescence with an exposure time of 10 seconds..

All lanes:

Immunoprecipitation - Anti-Pericentrin 1/FROUNT antibody (ab176801)

Predicted band size: 75 kDa

false

Supplier Data

Western blot - Anti-Pericentrin 1/FROUNT antibody (AB176801)

All lanes:

Western blot - Anti-Pericentrin 1/FROUNT antibody (ab176801) at 0.1 µg/mL

Lane 1:

293T whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 50 µg

Lane 3:

Jurkat whole cell lysate at 50 µg

Lane 4:

TCMK-1 whole cell lysate at 50 µg

Lane 5:

NIH 3T3 whole cell lysate at 50 µg

Predicted band size: 75 kDa

true

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human NUP85 aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

Q9BW27

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cynomolgus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab176801 was affinity purified using an epitope specific to Pericentrin 1/FROUNT immobilized on solid support.

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Pericentrin also known as FROUNT is a protein that plays a critical role in organizing microtubule arrays inside cells. It exhibits a molecular mass of approximately 360-400 kDa. This protein localizes mainly at the centrosome an important cellular structure involved in microtubule nucleation as well as at basal bodies within cilia. In centrosomes Pericentrin serves as an anchoring point for several other proteins which helps in maintaining centrosome integrity and supporting cell division processes.

Biological function summary

Pericentrin helps coordinate the microtubule network facilitating essential cellular events such as mitosis and intracellular transport. As part of a larger protein complex Pericentrin interacts with gamma-tubulin and several other centrosomal proteins to enhance microtubule nucleation. Additionally Pericentrin helps stabilize the microtubule organizing center ensuring proper cell cycle progression and cellular architecture.

Pathways

Pericentrin plays a role in the centrosome cycle and mitotic spindle assembly. It interacts with proteins like CEP152 and CDK5RAP2 which are important for proper centrosome duplication and function. These interactions contribute to pathways involved in cell division particularly the regulation of the mitotic spindle and chromosome stability during cell division.

Pericentrin mutations or dysfunctions have been linked to conditions such as microcephalic osteodysplastic primordial dwarfism and certain cancers. Its role in centrosome integrity ties it to BRCA1 a protein associated with breast and ovarian cancer susceptibility. Disrupted Pericentrin function can lead to abnormal cell division and aneuploidy which are common features in cancerous growths.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Essential component of the nuclear pore complex (NPC) that seems to be required for NPC assembly and maintenance (PubMed : 12718872). As part of the NPC Nup107-160 subcomplex plays a role in RNA export and in tethering NUP96/Nup98 and NUP153 to the nucleus (PubMed : 12718872). The Nup107-160 complex seems to be required for spindle assembly during mitosis (PubMed : 16807356). NUP85 is required for membrane clustering of CCL2-activated CCR2 (PubMed : 15995708). Seems to be involved in CCR2-mediated chemotaxis of monocytes and may link activated CCR2 to the phosphatidyl-inositol 3-kinase-Rac-lammellipodium protrusion cascade (PubMed : 15995708). Involved in nephrogenesis (PubMed : 30179222).

See full target information NUP85

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:6588 PubMed40675960

2025

Regulation of plant immunity through histone H3 β-hydroxybutyrylation-mediated transcriptional control.

Applications

Unspecified application

Species

Unspecified reactive species

Qiutao Xu,Zhengting Chen,Rui Wang,Xuan Ma,Yuhang Duan,Du Hai,Jing Chen,Zhongyi Cheng,Lu Zheng,Junbin Huang,Jisen Zhang,Yu Zhao,Xiaoyang Chen

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Pericentrin 1/FROUNT antibody

Immunoprecipitation - Anti-Pericentrin 1/FROUNT antibody (AB176801)

Western blot - Anti-Pericentrin 1/FROUNT antibody (AB176801)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Complementary Products

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise