AB231958

Anti-Pericentrin 1/FROUNT antibody

Name: Anti-Pericentrin 1/FROUNT antibody (ab231958) | Abcam
Brand: Abcam Limited
SKU: AB231958
Price: 525 USD
Availability: InStock

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(2 Publications)

Rabbit Polyclonal Pericentrin 1/FROUNT antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Mouse Nup85 aa 400 to C-terminus.

View Alternative Names

NUP75, PCNT1, NUP85, Nuclear pore complex protein Nup85, 85 kDa nucleoporin, FROUNT, Nucleoporin Nup75, Nucleoporin Nup85, Pericentrin-1

6 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pericentrin 1/FROUNT antibody (AB231958)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pericentrin 1/FROUNT antibody (AB231958)

Formalin-fixed, paraffin-embedded mouse testis tissue stained for Pericentrin 1/FROUNT using ab231958 at 20 μg/ml in immunohistochemical analysis. DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pericentrin 1/FROUNT antibody (AB231958)

Formalin-fixed, paraffin-embedded mouse heart tissue stained for Pericentrin 1/FROUNT using ab231958 at 20 μg/ml in immunohistochemical analysis. DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pericentrin 1/FROUNT antibody (AB231958)

Formalin-fixed, paraffin-embedded mouse uterus tissue stained for Pericentrin 1/FROUNT using ab231958 at 20 μg/ml in immunohistochemical analysis. DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pericentrin 1/FROUNT antibody (AB231958)

Formalin-fixed, paraffin-embedded mouse stomach tissue stained for Pericentrin 1/FROUNT using ab231958 at 20 μg/ml in immunohistochemical analysis. DAB staining.

Supplier Data

Western blot - Anti-Pericentrin 1/FROUNT antibody (AB231958)

All lanes:

Western blot - Anti-Pericentrin 1/FROUNT antibody (ab231958) at 2 µg/mL

All lanes:

Recombinant human Pericentrin 1/FROUNT protein

Secondary

All lanes:

HRP-Linked Guinea pig Anti-Rabbit at 1/2000 dilution

Predicted band size: 75 kDa

false

Supplier Data

Western blot - Anti-Pericentrin 1/FROUNT antibody (AB231958)

All lanes:

Western blot - Anti-Pericentrin 1/FROUNT antibody (ab231958) at 2 µg/mL

Lane 1:

Mouse lung tissue lysate

Lane 2:

Mouse liver tissue lysate

Lane 3:

Mouse heart tissue lysate

Secondary

All lanes:

HRP-Linked Guinea pig Anti-Rabbit at 1/2000 dilution

Predicted band size: 75 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Mouse Nup85 aa 400 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q8R480

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-2 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-2 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5-20 µg/mL", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Purification notes

Antigen-specific affinity chromatography followed by Protein A affinity chromatography.

Storage buffer

pH: 7.4 Preservative: 0.011% Proclin 300 Constituents: PBS, 55.77% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Pericentrin also known as FROUNT is a protein that plays a critical role in organizing microtubule arrays inside cells. It exhibits a molecular mass of approximately 360-400 kDa. This protein localizes mainly at the centrosome an important cellular structure involved in microtubule nucleation as well as at basal bodies within cilia. In centrosomes Pericentrin serves as an anchoring point for several other proteins which helps in maintaining centrosome integrity and supporting cell division processes.

Biological function summary

Pericentrin helps coordinate the microtubule network facilitating essential cellular events such as mitosis and intracellular transport. As part of a larger protein complex Pericentrin interacts with gamma-tubulin and several other centrosomal proteins to enhance microtubule nucleation. Additionally Pericentrin helps stabilize the microtubule organizing center ensuring proper cell cycle progression and cellular architecture.

Pathways

Pericentrin plays a role in the centrosome cycle and mitotic spindle assembly. It interacts with proteins like CEP152 and CDK5RAP2 which are important for proper centrosome duplication and function. These interactions contribute to pathways involved in cell division particularly the regulation of the mitotic spindle and chromosome stability during cell division.

Pericentrin mutations or dysfunctions have been linked to conditions such as microcephalic osteodysplastic primordial dwarfism and certain cancers. Its role in centrosome integrity ties it to BRCA1 a protein associated with breast and ovarian cancer susceptibility. Disrupted Pericentrin function can lead to abnormal cell division and aneuploidy which are common features in cancerous growths.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Essential component of the nuclear pore complex (NPC) that seems to be required for NPC assembly and maintenance (PubMed : 12718872). As part of the NPC Nup107-160 subcomplex plays a role in RNA export and in tethering NUP96/Nup98 and NUP153 to the nucleus (PubMed : 12718872). The Nup107-160 complex seems to be required for spindle assembly during mitosis (PubMed : 16807356). NUP85 is required for membrane clustering of CCL2-activated CCR2 (PubMed : 15995708). Seems to be involved in CCR2-mediated chemotaxis of monocytes and may link activated CCR2 to the phosphatidyl-inositol 3-kinase-Rac-lammellipodium protrusion cascade (PubMed : 15995708). Involved in nephrogenesis (PubMed : 30179222).

See full target information NUP85

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Molecular genetics & genomic medicine 13:e70053 PubMed39924725

2025

Effects of a Novel COL4A3 Homozygous/Heterozygous Splicing Mutation on the Mild Phenotype in a Family With Autosomal Recessive Alport Syndrome and a Literature Review.

Applications

Unspecified application

Species

Unspecified reactive species

Dan Chen,Li Zhang,Jing Rao,Yan Zhou,Lujun Dai,Songsong Huang,Chunxia Yang,Qiuhan Bian,Tao Zhang,Xiaoyan Yang

Frontiers in molecular biosciences 9:805235 PubMed35300115

2022

Integrated Strategies of Diverse Feature Selection Methods Identify Aging-Based Reliable Gene Signatures for Ischemic Cardiomyopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Huafeng Song,Shaoze Chen,Tingting Zhang,Xiaofei Huang,Qiyu Zhang,Cuizhi Li,Chunlin Chen,Shaoxian Chen,Dehui Liu,Jiawen Wang,Yingfeng Tu,Yueheng Wu,Youbin Liu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Pericentrin 1/FROUNT antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pericentrin 1/FROUNT antibody (AB231958)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pericentrin 1/FROUNT antibody (AB231958)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pericentrin 1/FROUNT antibody (AB231958)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pericentrin 1/FROUNT antibody (AB231958)

Western blot - Anti-Pericentrin 1/FROUNT antibody (AB231958)

Western blot - Anti-Pericentrin 1/FROUNT antibody (AB231958)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Complementary Products

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise