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AB99341

Anti-Pericentrin antibody

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(2 Publications)

Rabbit Polyclonal Pericentrin antibody. Suitable for ICC, IP, WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human PCNT aa 150-250.

View Alternative Names

KIAA0402, PCNT2, PCNT, Pericentrin, Kendrin, Pericentrin-B

3 Images
Immunocytochemistry - Anti-Pericentrin antibody (AB99341)
  • ICC

Unknown

Immunocytochemistry - Anti-Pericentrin antibody (AB99341)

Immunocytochemistry/Immunofluorescence analysis of formaldehyde-fixed asynchronous HeLa cells labelling Pericentrin with ab99341 at 1/1000 (1µg/ml). A DyLight® 594-conjugated goat anti-rabbit IgG (H+L) (1/100) was used as the secondary antibody.

Immunoprecipitation - Anti-Pericentrin antibody (AB99341)
  • IP

Unknown

Immunoprecipitation - Anti-Pericentrin antibody (AB99341)

Detection of Pericentrin by Western Blot of Immunoprecipitates in HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) using ab99341 at 3 µg/mg lysate. Anti-Pericentrin antibody ab99342, which recognizes a downstream epitope, was used at 1 µg/ml for subsequent WB detection.

All lanes:

Immunoprecipitation - Anti-Pericentrin antibody (ab99341)

Predicted band size: 378 kDa

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Western blot - Anti-Pericentrin antibody (AB99341)
  • WB

Supplier Data

Western blot - Anti-Pericentrin antibody (AB99341)

Detection : Chemiluminescence with an exposure time of 30 seconds.

All lanes:

Western blot - Anti-Pericentrin antibody (ab99341) at 0.1 µg/mL

Lane 1:

HeLa cell lysate prepared using NETN lysis buffer at 50 µg

Lane 2:

HEK293T cell lysate prepared using NETN lysis buffer at 50 µg

Predicted band size: 378 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, ICC, IP

applications

Immunogen

Synthetic Peptide within Human PCNT aa 150-250. The exact immunogen used to generate this antibody is proprietary information.

O95613

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "1/1000 - 1/5000", "ICC-species-notes": "<p>Formaldehyde or Acetone fixation is recommended. Permeabilization with Triton-X 100 is recommended for formaldehydefixed cells.</p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris citrate/phosphate
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Pericentrin also known as PCNT is a large protein with a mass of approximately 360 kDa. It primarily localizes at the centrosome an organelle serving as the main microtubule organizing center in cells. Expressed in most cell types pericentrin is an essential structural component of the centrosome. Researchers often use pericentrin staining techniques to visualize centrosomes aiding in the study of cell division. This protein functions prominently as a centrosome marker facilitating centrosome image and imaging of centrosome-related structures.
Biological function summary

Pericentrin contributes significantly to maintaining centrosome integrity and organizing microtubules during cell division. It is a part of the pericentriolar material a complex that stabilizes and anchors microtubules to the centrosome. By forming a scaffold at the centrosome pericentrin plays an important role in recruiting other proteins like γ-tubulin enhancing its function in nucleating microtubules. The pericentrin protein is vital for proper spindle organization during mitosis affecting accurate chromosome segregation.

Pathways

Pericentrin is integral to the cell cycle and mitotic spindle assembly pathways. It interacts with several other proteins involved in mitosis including γ-tubulin and ninein to manage the assembly and stability of the mitotic spindle. By participating in these pathways pericentrin ensures correct cellular replication and division therefore maintaining genetic stability within organisms.

Mutations in the pericentrin gene have been linked to microcephalic osteodysplastic primordial dwarfism type II and Seckel syndrome. These conditions suggest a connection between defective pericentrin function and disruptions in cell division and growth. Studies also show that alterations in pericentrin levels can influence tumorigenesis by affecting centrosome function and chromosome segregation increasing the importance of this protein in cancer research.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Integral component of the filamentous matrix of the centrosome involved in the initial establishment of organized microtubule arrays in both mitosis and meiosis. Plays a role, together with DISC1, in the microtubule network formation. Is an integral component of the pericentriolar material (PCM). May play an important role in preventing premature centrosome splitting during interphase by inhibiting NEK2 kinase activity at the centrosome.
See full target information PCNT
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

PloS one 19:e0307154 PubMed39093886

2024

Valproic acid use is associated with diminished risk of contracting COVID-19, and diminished disease severity: Epidemiologic and in vitro analysis reveal mechanistic insights.

Applications

Unspecified application

Species

Unspecified reactive species

Amanda Watson,Pankil Shah,Doug Lee,Sitai Liang,Geeta Joshi,Ediri Metitiri,Wasim H Chowdhury,Dean Bacich,Peter Dube,Yan Xiang,Daniel Hanley,Luis Martinez-Sobrido,Ronald Rodriguez

International journal of biological sciences 18:4329-4340 PubMed35864961

2022

PRMT5 acts as a tumor suppressor by inhibiting Wnt/β-catenin signaling in murine gastric tumorigenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Yuling Tang,Lei Dong,Chong Zhang,Xiubin Li,Rongyu Li,Huisang Lin,Yini Qi,Mingchuan Tang,Yanli Peng,Chuan Liu,Jian Zhou,Ning Hou,Wenjia Liu,Guan Yang,Xiao Yang,Yan Teng
View all publications
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Product promise

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