Anti-Pericentrin antibody ab4448 is a rabbit polyclonal antibody that is used in Pericentrin immunofluorescence. Suitable for human and mouse samples.
- Tried and trusted by researchers since 2004
IgG
Rabbit
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA
Liquid
Polyclonal
ICC/IF | |
---|---|
Human | Tested |
Mouse | Tested |
Rat | Predicted |
African green monkey | Predicted |
Rabbit | Predicted |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 0.1-0.5 µg/mL | Notes - |
Species Human | Dilution info 0.1-0.5 µg/mL | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rabbit, African green monkey, Rat | Dilution info - | Notes - |
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Integral component of the filamentous matrix of the centrosome involved in the initial establishment of organized microtubule arrays in both mitosis and meiosis. Plays a role, together with DISC1, in the microtubule network formation. Is an integral component of the pericentriolar material (PCM). May play an important role in preventing premature centrosome splitting during interphase by inhibiting NEK2 kinase activity at the centrosome.
Pcnt2, Pericentrin
Anti-Pericentrin antibody ab4448 is a rabbit polyclonal antibody that is used in Pericentrin immunofluorescence. Suitable for human and mouse samples.
- Tried and trusted by researchers since 2004
IgG
Rabbit
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA
Liquid
Polyclonal
Affinity purification Protein G
This antibody should recognise both Pericentrin and Kendrin (also known as Pericentrin-2).
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
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This supplementary information is collated from multiple sources and compiled automatically.
Pericentrin also known as PCNT is a large protein with a mass of approximately 360 kDa. It primarily localizes at the centrosome an organelle serving as the main microtubule organizing center in cells. Expressed in most cell types pericentrin is an essential structural component of the centrosome. Researchers often use pericentrin staining techniques to visualize centrosomes aiding in the study of cell division. This protein functions prominently as a centrosome marker facilitating centrosome image and imaging of centrosome-related structures.
Pericentrin contributes significantly to maintaining centrosome integrity and organizing microtubules during cell division. It is a part of the pericentriolar material a complex that stabilizes and anchors microtubules to the centrosome. By forming a scaffold at the centrosome pericentrin plays an important role in recruiting other proteins like γ-tubulin enhancing its function in nucleating microtubules. The pericentrin protein is vital for proper spindle organization during mitosis affecting accurate chromosome segregation.
Pericentrin is integral to the cell cycle and mitotic spindle assembly pathways. It interacts with several other proteins involved in mitosis including γ-tubulin and ninein to manage the assembly and stability of the mitotic spindle. By participating in these pathways pericentrin ensures correct cellular replication and division therefore maintaining genetic stability within organisms.
Mutations in the pericentrin gene have been linked to microcephalic osteodysplastic primordial dwarfism type II and Seckel syndrome. These conditions suggest a connection between defective pericentrin function and disruptions in cell division and growth. Studies also show that alterations in pericentrin levels can influence tumorigenesis by affecting centrosome function and chromosome segregation increasing the importance of this protein in cancer research.
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A representative panel of indirect immunofluorescence microscopic images shows normal (regular, n ≤ 2) and aberrant centrosome numbers (n > 2) in interphase cells.
Centrosomes were stained using anti-pericentrin antibody ab4448 (magenta), nuclear DNA is shown in blue (DAPI).
Statistical methods: Kruskal-Wallis test. Mann-Whitney U tests followed by Bonferroni-Holm p-value correction were made as post-hoc tests in order to compare the MDS and sAML patients with the control group.
ab4448 staining Pericentrin in HeLa cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab4448 at 0.1µg/ml and Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was acquired with a confocal microscope (Leica-Microsystems TCS SP8) and a single confocal section is shown.
ab4448 staining Pericentrin in NIH3T3 cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab4448 at 0.1µg/ml and Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).
Also suitable in cells fixed with 100% methanol (5 min).
Image was acquired with a confocal microscope (Leica-Microsystems TCS SP8) and a single confocal section is shown.
IF staining of pericentrin in MCF7 (Human breast adenocarcinoma cell line) cells.
The top panel is an interphase cell showing centrosome staining.
The bottom panel shows a mitotic cell with spindle pole staining.
ab4448 was used at 1/500, but also works at higher dilutions (1/1000-1/2000).
Top panel - 630X magnification; Bottom panel -1000X magnification.
The secondary antibody was Alexa-Fluor®488 anti-rabbit.
NIH/3T3 (Mouse embryo fibroblast cell line) cells were fixed in 100% methanol for 6 minutes at -20°C, washed 3 times in PBS then incubated with ab4448 (1/2000) for 1 hour at room temperature.
The panel of images shows the nuclei stained with DAPI (blue), ab4448 staining is shown in green. 100x magnification.
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