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Anti-Pericentrin antibody ab4448 is a rabbit polyclonal antibody that is used in Pericentrin immunofluorescence. Suitable for human and mouse samples.

- Tried and trusted by researchers since 2004


Images

Immunocytochemistry/ Immunofluorescence - Anti-Pericentrin antibody - Centrosome Marker (AB4448), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Pericentrin antibody - Centrosome Marker (AB4448), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Pericentrin antibody - Centrosome Marker (AB4448), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Pericentrin antibody - Centrosome Marker (AB4448), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Pericentrin antibody - Centrosome Marker (AB4448), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA

Form

Liquid

Clonality

Polyclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IF
Human
Tested
Mouse
Tested
Rat
Predicted
African green monkey
Predicted
Rabbit
Predicted

Tested
Tested

Species

Mouse

Dilution info

0.1-0.5 µg/mL

Notes

-

Species

Human

Dilution info

0.1-0.5 µg/mL

Notes

-

Predicted
Predicted

Species

Rabbit, African green monkey, Rat

Dilution info

-

Notes

-

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Target data

Function

Integral component of the filamentous matrix of the centrosome involved in the initial establishment of organized microtubule arrays in both mitosis and meiosis. Plays a role, together with DISC1, in the microtubule network formation. Is an integral component of the pericentriolar material (PCM). May play an important role in preventing premature centrosome splitting during interphase by inhibiting NEK2 kinase activity at the centrosome.

Alternative names

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Anti-Pericentrin antibody ab4448 is a rabbit polyclonal antibody that is used in Pericentrin immunofluorescence. Suitable for human and mouse samples.

- Tried and trusted by researchers since 2004

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Purification technique

Affinity purification Protein G

Specificity

This antibody should recognise both Pericentrin and Kendrin (also known as Pericentrin-2).

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Pericentrin also known as PCNT is a large protein with a mass of approximately 360 kDa. It primarily localizes at the centrosome an organelle serving as the main microtubule organizing center in cells. Expressed in most cell types pericentrin is an essential structural component of the centrosome. Researchers often use pericentrin staining techniques to visualize centrosomes aiding in the study of cell division. This protein functions prominently as a centrosome marker facilitating centrosome image and imaging of centrosome-related structures.

Biological function summary

Pericentrin contributes significantly to maintaining centrosome integrity and organizing microtubules during cell division. It is a part of the pericentriolar material a complex that stabilizes and anchors microtubules to the centrosome. By forming a scaffold at the centrosome pericentrin plays an important role in recruiting other proteins like γ-tubulin enhancing its function in nucleating microtubules. The pericentrin protein is vital for proper spindle organization during mitosis affecting accurate chromosome segregation.

Pathways

Pericentrin is integral to the cell cycle and mitotic spindle assembly pathways. It interacts with several other proteins involved in mitosis including γ-tubulin and ninein to manage the assembly and stability of the mitotic spindle. By participating in these pathways pericentrin ensures correct cellular replication and division therefore maintaining genetic stability within organisms.

Associated diseases and disorders

Mutations in the pericentrin gene have been linked to microcephalic osteodysplastic primordial dwarfism type II and Seckel syndrome. These conditions suggest a connection between defective pericentrin function and disruptions in cell division and growth. Studies also show that alterations in pericentrin levels can influence tumorigenesis by affecting centrosome function and chromosome segregation increasing the importance of this protein in cancer research.

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5 product images

  • Immunocytochemistry/ Immunofluorescence - Anti-Pericentrin antibody - Centrosome Marker (ab4448), expandable thumbnail
    Ruppenthal et al PLoS One. 2018 Jan 25;13(1):e0191734. doi: 10.1371/journal.pone.0191734. eCollection 2018. Fig 2. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

    Immunocytochemistry/ Immunofluorescence - Anti-Pericentrin antibody - Centrosome Marker (ab4448)

    A representative panel of indirect immunofluorescence microscopic images shows normal (regular, n ≤ 2) and aberrant centrosome numbers (n > 2) in interphase cells.

    Centrosomes were stained using anti-pericentrin antibody ab4448 (magenta), nuclear DNA is shown in blue (DAPI).

    Statistical methods: Kruskal-Wallis test. Mann-Whitney U tests followed by Bonferroni-Holm p-value correction were made as post-hoc tests in order to compare the MDS and sAML patients with the control group.

  • Immunocytochemistry/ Immunofluorescence - Anti-Pericentrin antibody - Centrosome Marker (ab4448), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Pericentrin antibody - Centrosome Marker (ab4448)

    ab4448 staining Pericentrin in HeLa cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab4448 at 0.1µg/ml and Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was acquired with a confocal microscope (Leica-Microsystems TCS SP8) and a single confocal section is shown.

  • Immunocytochemistry/ Immunofluorescence - Anti-Pericentrin antibody - Centrosome Marker (ab4448), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Pericentrin antibody - Centrosome Marker (ab4448)

    ab4448 staining Pericentrin in NIH3T3 cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab4448 at 0.1µg/ml and Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).

    Also suitable in cells fixed with 100% methanol (5 min).

    Image was acquired with a confocal microscope (Leica-Microsystems TCS SP8) and a single confocal section is shown.

  • Immunocytochemistry/ Immunofluorescence - Anti-Pericentrin antibody - Centrosome Marker (ab4448), expandable thumbnail
    This image is courtesy of Gordon Chan, University of Alberta

    Immunocytochemistry/ Immunofluorescence - Anti-Pericentrin antibody - Centrosome Marker (ab4448)

    IF staining of pericentrin in MCF7 (Human breast adenocarcinoma cell line) cells.

    The top panel is an interphase cell showing centrosome staining.

    The bottom panel shows a mitotic cell with spindle pole staining.

    ab4448 was used at 1/500, but also works at higher dilutions (1/1000-1/2000).

    Top panel - 630X magnification; Bottom panel -1000X magnification.

    The secondary antibody was Alexa-Fluor®488 anti-rabbit.

  • Immunocytochemistry/ Immunofluorescence - Anti-Pericentrin antibody - Centrosome Marker (ab4448), expandable thumbnail
    This image is courtesy of Roberto Giambruno, Marilena Ciciarello and Patrizia Lavia

    Immunocytochemistry/ Immunofluorescence - Anti-Pericentrin antibody - Centrosome Marker (ab4448)

    NIH/3T3 (Mouse embryo fibroblast cell line) cells were fixed in 100% methanol for 6 minutes at -20°C, washed 3 times in PBS then incubated with ab4448 (1/2000) for 1 hour at room temperature.

    The panel of images shows the nuclei stained with DAPI (blue), ab4448 staining is shown in green. 100x magnification.

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