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Anti-Pericentrin antibody [EPR21987] - Centrosome Marker (ab220784) is a rabbit monoclonal antibody detecting Pericentrin in Flow Cytometry (Intra), Flow Cytometry, IP, IHC-P, ICC/IF. Suitable for Human, Mouse.



- Biophysical QC for unrivalled batch-batch consistency


Images

Immunoprecipitation - Anti-Pericentrin antibody [EPR21987] - Centrosome Marker (AB220784), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Pericentrin antibody [EPR21987] - Centrosome Marker (AB220784), expandable thumbnail
  • Immunoprecipitation - Anti-Pericentrin antibody [EPR21987] - Centrosome Marker (AB220784), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Pericentrin antibody [EPR21987] - Centrosome Marker (AB220784), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-Pericentrin antibody [EPR21987] - Centrosome Marker (AB220784), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPICC/IFFlow Cyt (Intra)IHC-P
Human
Tested
Tested
Tested
Tested
Mouse
Tested
Tested
Expected
Expected

Tested
Tested

Species
Mouse
Dilution info
1/30
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Human
Dilution info
1/30
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/2000
Notes

-

Species
Human
Dilution info
1/2000
Notes

-

Tested
Tested

Species
Human
Dilution info
1/60
Notes

-

Expected
Expected

Species
Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
1/2000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species
Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Associated Products

Select an associated product type

3 products for Alternative Product

Target data

Function

Integral component of the filamentous matrix of the centrosome involved in the initial establishment of organized microtubule arrays in both mitosis and meiosis. Plays a role, together with DISC1, in the microtubule network formation. Is an integral component of the pericentriolar material (PCM). May play an important role in preventing premature centrosome splitting during interphase by inhibiting NEK2 kinase activity at the centrosome.

Alternative names

Recommended products

Anti-Pericentrin antibody [EPR21987] - Centrosome Marker (ab220784) is a rabbit monoclonal antibody detecting Pericentrin in Flow Cytometry (Intra), Flow Cytometry, IP, IHC-P, ICC/IF. Suitable for Human, Mouse.



- Biophysical QC for unrivalled batch-batch consistency

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR21987
Purification technique
Affinity purification Protein A
Specificity

IHC is recommended for human only.

Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

What is this antibody validated in?


Anti-Pericentrin antibody [EPR21987] - Centrosome Marker (ab220784) is a rabbit recombinant monoclonal antibody and is validated for use in Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse samples.

Trial sizes available!


Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Other related products


We have a range of other formats of antibody clone [EPR21987] also available for your convenience:
ab220784, Carrier free - Anti-Pericentrin antibody [EPR21987] - BSA and Azide free ab237034, Alexa Fluor® 488 - Alexa Fluor® 488 Anti-Pericentrin antibody [EPR21987] - Centrosome Marker ab270118, Alexa Fluor® 647 - Alexa Fluor® 647 Anti-Pericentrin antibody [EPR21987] - Centrosome Marker ab270119, Alexa Fluor® 594 - Alexa Fluor® 594 Anti-Pericentrin - Centrosome Marker antibody [EPR21987] ab314881, Alexa Fluor® 555 - Alexa Fluor® 555 Anti-Pericentrin antibody [EPR21987] - Centrosome Marker ab319181, PE - PE Anti-Pericentrin antibody [EPR21987] - Centrosome Marker ab322452



Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Pericentrin also known as PCNT is a large protein with a mass of approximately 360 kDa. It primarily localizes at the centrosome an organelle serving as the main microtubule organizing center in cells. Expressed in most cell types pericentrin is an essential structural component of the centrosome. Researchers often use pericentrin staining techniques to visualize centrosomes aiding in the study of cell division. This protein functions prominently as a centrosome marker facilitating centrosome image and imaging of centrosome-related structures.

Biological function summary

Pericentrin contributes significantly to maintaining centrosome integrity and organizing microtubules during cell division. It is a part of the pericentriolar material a complex that stabilizes and anchors microtubules to the centrosome. By forming a scaffold at the centrosome pericentrin plays an important role in recruiting other proteins like γ-tubulin enhancing its function in nucleating microtubules. The pericentrin protein is vital for proper spindle organization during mitosis affecting accurate chromosome segregation.

Pathways

Pericentrin is integral to the cell cycle and mitotic spindle assembly pathways. It interacts with several other proteins involved in mitosis including γ-tubulin and ninein to manage the assembly and stability of the mitotic spindle. By participating in these pathways pericentrin ensures correct cellular replication and division therefore maintaining genetic stability within organisms.

Associated diseases and disorders

Mutations in the pericentrin gene have been linked to microcephalic osteodysplastic primordial dwarfism type II and Seckel syndrome. These conditions suggest a connection between defective pericentrin function and disruptions in cell division and growth. Studies also show that alterations in pericentrin levels can influence tumorigenesis by affecting centrosome function and chromosome segregation increasing the importance of this protein in cancer research.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

8 product images

  • Immunoprecipitation - Anti-Pericentrin antibody [EPR21987] - Centrosome Marker (ab220784), expandable thumbnail

    Immunoprecipitation - Anti-Pericentrin antibody [EPR21987] - Centrosome Marker (ab220784)

    Pericentrin was immunoprecipitated from 0.35 mg HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate with ab220784 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab220784 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/1000 dilution.

    Lane 1: HepG2 whole cell lysate 10 μg (input).
    Lane 2: ab220784 IP in HepG2 whole cell lysate (+).
    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab220784 in HepG2 whole cell lysate (-).

    Blocking and dilution buffer and concentration: 5% NFDM/TBST
    Exposure time: 30 seconds

    The 220 kDa band represent an isoform of Pericentrin (PMID: 23060948). We used a high sensitivity ECL substrate to develop this blot.

    All lanes: Immunoprecipitation - Anti-Pericentrin antibody [EPR21987] - Centrosome Marker (ab220784)

    Predicted band size: 378 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-Pericentrin antibody [EPR21987] - Centrosome Marker (ab220784), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Pericentrin antibody [EPR21987] - Centrosome Marker (ab220784)

    Immunofluorescent analysis of 100% Methanol-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling Pericentrin with ab220784 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing centrosome staining in HeLa cell line.

    The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889) (red) at 1/200 dilution.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution.

  • Immunoprecipitation - Anti-Pericentrin antibody [EPR21987] - Centrosome Marker (ab220784), expandable thumbnail

    Immunoprecipitation - Anti-Pericentrin antibody [EPR21987] - Centrosome Marker (ab220784)

    Pericentrin was immunoprecipitated from 0.35 mg NIH/3T3 (mouse embryonic fibroblast cell line) whole cell lysate with ab220784 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab220784 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection 1/1000 dilution.

    Lane 1: NIH/3T3 whole cell lysate 10 μg (input).
    Lane 2: ab220784 IP in NIH/3T3 whole cell lysate (+).
    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab220784 in NIH/3T3 whole cell lysate (-).

    Blocking and dilution buffer and concentration: 5% NFDM/TBST
    Exposure time: 30 seconds

    The 220 kDa band represent an isoform of Pericentrin (PMID: 23060948). We use high sensitive ECL substrate.

    All lanes: Immunoprecipitation - Anti-Pericentrin antibody [EPR21987] - Centrosome Marker (ab220784)

    Predicted band size: 378 kDa

    Observed band size: 220 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-Pericentrin antibody [EPR21987] - Centrosome Marker (ab220784), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Pericentrin antibody [EPR21987] - Centrosome Marker (ab220784)

    Immunofluorescent analysis of 100% Methanol-fixed NIH/3T3 (mouse embyro fibroblast cell line) cells labeling Pericentrin with ab220784 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing centrosome staining in NIH/3T3 cell line.

    The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889) (red) at 1/200 dilution.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution.

  • Flow Cytometry (Intracellular) - Anti-Pericentrin antibody [EPR21987] - Centrosome Marker (ab220784), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-Pericentrin antibody [EPR21987] - Centrosome Marker (ab220784)

    Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cell line labeling Pericentrin with ab220784 at 1/60 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (black) and an unlabeled control (cells incubated with secondary antibody only) (blue). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as the secondary antibody.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pericentrin antibody [EPR21987] - Centrosome Marker (ab220784), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pericentrin antibody [EPR21987] - Centrosome Marker (ab220784)

    Immunohistochemical analysis of paraffin-embedded human breast tissue labeling Pericentin with ab220784 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining of centrosomes in human mammary epithelial cells (PMID: 16534625, PMID: 22031837). Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pericentrin antibody [EPR21987] - Centrosome Marker (ab220784), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pericentrin antibody [EPR21987] - Centrosome Marker (ab220784)

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue labeling Pericentin with ab220784 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining of centrosomes in human pancreas (PMID: 16534625, PMID: 22031837). Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pericentrin antibody [EPR21987] - Centrosome Marker (ab220784), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pericentrin antibody [EPR21987] - Centrosome Marker (ab220784)

    Immunohistochemical analysis of paraffin-embedded human thyroid carcinoma tissue labeling Pericentin with ab220784 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining of centrosomes in human thyroid carcinoma (PMID: 16534625, PMID: 22031837). Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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