Anti-Pericentrin antibody [mAbcam 28144] - Centrosome Marker

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AbReview20895****

Immunocytochemistry/ Immunofluorescence - Anti-Pericentrin antibody [mAbcam 28144] - Centrosome Marker (AB28144)

Immunocytochemistry/ Immunofluorescence analysis of HeLa cells labeling Pericentrin with ab28144 at 1/200 dilution. Cells were fixed with methanol, followed by incubation with Anti-Pericentrin antibody [mAbcam 28144] - Centrosome Marker (ab28144) in PBS for 1 hour at 22°C. A polyclonal goat anti-mouse Cy3^® secondary antibody was used at 1/200 dilution. Nuclear counterstained with DAPI.

This image was generated from the hybridoma version.

This image is courtesy of an Abreview submitted by Dr Kirk McManus, Univ. of Manitoba/Cancer Care MICB.

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AbReview14379****

Immunocytochemistry/ Immunofluorescence - Anti-Pericentrin antibody [mAbcam 28144] - Centrosome Marker (AB28144)

Immunocytochemistry/ Immunofluorescence analysis of A549 lung cells labeling Pericentrin with ab28144 at 1/100 dilution. Cells were fixed with formaldehyde and permeabilized with Trixton X-100. The cells were blocked with 10% serum for 30 minutes at 20°C, followed by incubation with ab28144 in PBS 10% FCS for 1 hour at 20°C. A polyclonal goat anti-mouse Alexa Fluor^® 594 secondary antibody was used at 1/300 dilution. This image was generated from the hybridoma version.

This image is courtesy of an anonymous abreview.

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PubMed

Immunocytochemistry/ Immunofluorescence - Anti-Pericentrin antibody [mAbcam 28144] - Centrosome Marker (AB28144)

Immunocytochemistry/ Immunofluorescence analysis of Y79 cells labeling Pericentrin with ab28144 (red). Cells were fixed for 10 minutes at room temperature in 4% paraformaldehyde. Cells were then permeabilized using 0.2% Triton/TBS and blocked with 5% NGS in 0.1% BSA/TBS-Tween. The cells were then incubated overnight at 4°C with Anti-Pericentrin antibody [mAbcam 28144] - Centrosome Marker (ab28144) at 1/1000 diluted in 0.1% BSA/TBS-Tween containing 1% NGS. Cells were then stained for Hoechst (blue) and alpha-tubulin (green).

This image was generated from the hybridoma version.

Image from Sikkema, William K. A. et al. PLoS ONE 9.6 (2014): e98838. PMC. Web. 24 Feb. 2017. doi: 10.1371/journal.pone.0098838. Fig 4F. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

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Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Pericentrin antibody [mAbcam 28144] - Centrosome Marker (AB28144)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HeLa (human cervical adenocarcinoma epithelial cell) cells labelling PCM1 with ab324615 at 1/5000 (0.1 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/ab150081 1000 2ug/ml dilution (Green).

Confocal image showing centrosome staining in HeLa cell line (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab28144 Anti-Pericentrin mouse monoclonal antibody - Centrosome Marker was used to counterstain tubulin at 1/ab28144 100 10.00ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/ab150081 1000 2ug/ml dilution.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Pericentrin antibody [mAbcam 28144] - Centrosome Marker (AB28144)

Immunocytochemical analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling Pericentrin with ab28144 at 1/50 dilution at 4°C overnight, followed by AlexaFluor^® 488-conjugated Goat anti-Rabbit secondary
antibody (ab150077) at 1/1000 dilution at RT for 30min. Alexa Fluor^® 594 Anti-alpha Tubulin antibody [DM1A] (ab195889) was used as a counterstain at 1/200 dilution at RT for 30min. Nucleus were visualized using DAPI. The cells were 4% PFA fixed and permeabilized with 0.1% Triton X-100.

Confocal image showing centrosome staining in HeLa cells.