Anti-Perilipin-1 antibody

• ICC

Supplier Data

Immunocytochemistry - Anti-Perilipin-1 antibody (AB3526)

Immunocytochemistry analysis of 3T3-L1 cells (Mouse embryonic fibroblast cell line), 4% paraformaldehyde fixed (10 minutes) permeabilized with 0.1% Triton™ X-100 (10 minutes), and blocked with 1% BSA for (1 hour).

Perilipin A was labeled with ab3526 at 2 μg/ml in 0.1% BSA and incubated for 3 hours at room temperature with secondary antibody : Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate at a dilution of 1 : 2000 for 45 minutes at room temperature (Panel A : green).

Panel B : Nuclei were stained with DAPI (blue); Panel C : (red) F-actin stained with Alexa Fluor® 555 Rhodamine Phalloidin 1 : 300. Panel D : merged image showing cytosolic localization; Panel E : untreated; Panel F : control cells with no primary antibody.

• WB

Unknown

Western blot - Anti-Perilipin-1 antibody (AB3526)

Western blot detection of Perilipin-1 in 3T3-L1 cell extract using ab3526.

All lanes:

Western blot - Anti-Perilipin-1 antibody (ab3526)

Predicted band size: 56 kDa

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• IHC-Fr

CiteAb

Immunohistochemistry (Frozen sections) - Anti-Perilipin-1 antibody (AB3526)

Perilipin-1 immunohistochemistry-immunofluorescence using Anti-Perilipin-1 antibody ab3526. Publication image and figure legend from Kakuta, T., Sawada, K., et al., 2020, Sci Rep, Pubmed 32094398.

Post-transplant expression of perilipin A in transplanted parathyroid parenchymal tissues from secondary hyperparathyroidism patients. The transplants shown in Fig. 4 were examined for perilipin A expression. Bound anti-perilipin A antibody was fluorescently visualized with Alexa 488 (green) and bound anti-human cytoplasm antibody with Alexa 594 (red). These views were merged and are shown under Merged. As a negative staining control, the section was stained without the primary antibody and viewed with red fluorescence. The empty arrow indicates human transplants.

• WB

CiteAb

Western blot - Anti-Perilipin-1 antibody (AB3526)

Western Blotting using Anti-Perilipin-1 antibody, ab3526. Publication image from Corbet, C. et al., 2020, Nat Commun, 31974393. Legend direct from paper.

Acidosis-adapted cancer cells exhibit accumulation of lipid droplets.a Representative electron microscopy pictures depicting lipid droplet (LD) accumulation in acidosis-adapted SiHa and HCT-116 cancer cells. Scale bar : 2 µm. b, c Representative pictures of BODIPY 493/503- (b) and ORO-stained (c) LD in the same native and acidosis-adapted cancer cells. Scale bar : 20 µm. d Quantification of ORO-stained LD area in native and acidosis-adapted SiHa cells. e–g Abundance of the total pool of neutral lipids (e), SFA and MUFA in the neutral lipid fraction (f), and cholesteryl esters (g) in native and acidosis-adapted SiHa cells. h Representative immunoblotting for perilipins 1, 2, and 5 in native and acidosis-adapted cancer cells. i LD content in native and acidosis-adapted SiHa following transfection of PLIN2-targeting (or control) siRNA for 72 h. j Scheme depicting the pathways leading to neutral lipid accumulation and lipid droplet biogenesis; specific inhibitors are indicated in red. k–m LD content in native and acidosis-adapted SiHa cells following treatment with 15 µM A922500 (DGAT1i) or 10 µM PF-06424439 (DGAT2i) for 24 h (k), incubation in a medium supplemented with lipid-containing serum (FBS) or with charcoal-delipidated serum (DFBS) in presence or absence of an exogenous lipid concentrate (LC) for 24 h (l) or treatment with two distinct CD36 blocking antibodies for 24 h (m). Data are represented as mean ± SEM of three independent experiments (with ≥6 technical replicates). Significance was determined by Student’s t-test (d, e, g), by one-way ANOVA (k–m) or two-way ANOVA (i) with Bonferroni multiple-comparison analysis. ***p < 0.001; ns, not significant. Source data are provided as a Source Data file.

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• WB

CiteAb

Western blot - Anti-Perilipin-1 antibody (AB3526)

Perilipin-1 western blotting using Anti-Perilipin-1 antibody ab3526. Publication image and figure legend from Zhao, X., Gao, M., et al., 2015, PLoS One, Pubmed 25855981.

Plin1 expresses in atherosclerotic lesions.(A) Immunoblotting analysis of Plin1 expression in perivascular adipose tissue and in peritoneal macrophages isolated from Plin1-/- and wild-type mice. Incubation with modified LDL increases Plin1 expression in the macrophages. Anti-Plin1 antiserum from (Abcam, #ab3526) recognizes the C-terminal amino-acid residues of Plin1. (B) Unstained frozen section of arotic root of ApoE-/- mice. (C) The frozen section of arotic root in ApoE-/- mice was immunostained (Green) with the primary antibody against CD68, a macrophagemarker. (D) Immunostaining of Plin1 (Red). (E and F) Merged image shows colocalization of Plin1 and CD68 in atheroma. Boxed area in panel E (a-c) were magnified to show colocalization of Plin1 and CD68 (F, a-c) in macrophages in atherosclerotic lesion in ApoE-/- mice.

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