Anti-perilipin-1 antibody is a rabbit polyclonal antibody that is used in Plin1 western blot (WB), IHC, immunocytochemistry (ICC), and flow cytometry. Suitable for human, mouse, rat, and more samples.
- Cited in over 130 publications
- One antibody for all your perilipin-1 staining
- Trusted by researchers since 2003
Images
Publications
Filter by
- Cell death & disease 14:7762023Deubiquitinase USP1 enhances CCAAT/enhancer-binding protein beta (C/EBPβ) stability and accelerates adipogenesis and lipid accumulation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMyung Sup Kim,Jung-Hwan Baek,JinAh Lee,Aneesh Sivaraman,Kyeong Lee,Kyung-Hee ChunPubMed 38012162
- Journal of animal science and biotechnology 14:1412023Single-base editing in IGF2 improves meat production and intramuscular fat deposition in Liang Guang Small Spotted pigs.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTianqi Duo,Xiaohong Liu,Delin Mo,Yu Bian,Shufang Cai,Min Wang,Ruiqiang Li,Qi Zhu,Xian Tong,Ziyun Liang,Weilun Jiang,Shiyi Chen,Yaosheng Chen,Zuyong HePubMed 37919760
- Biotechnology journal 18:e23000982023Effect of radiation sterilization on the ability to induce adipose regeneration in vivo in decellularized adipose-derived matrix.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJiayi Feng,Jun Qi,Su Fu,Jie LuanPubMed 37449520
- Experimental & molecular medicine 55:1520-15302023Ablation of the deubiquitinase USP15 ameliorates nonalcoholic fatty liver disease and nonalcoholic steatohepatitis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJung-Hwan Baek,Myung Sup Kim,Hye Ryeon Jung,Min-Seon Hwang,Chan-Ho Lee,Dai Hoon Han,Yong-Ho Lee,Eugene C Yi,Seung-Soon Im,Ilseon Hwang,Kyungeun Kim,Joon-Yong Chung,Kyung-Hee ChunPubMed 37394587
- Bioengineering (Basel, Switzerland) 10:2023Selection of Mechanical Fragmentation Methods Based on Enzyme-Free Preparation of Decellularized Adipose-Derived Matrix.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJiayi Feng,Su Fu,Jie LuanPubMed 37508785
- International journal of nanomedicine 18:2923-29382023Preparation of Apoptotic Extracellular Vesicles from Adipose Tissue and Their Efficacy in Promoting High-Quality Skin Wound Healing.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJia Dong,Bin Wu,Weidong TianPubMed 37288352
- Nature communications 14:30202023Wound infiltrating adipocytes are not myofibroblasts.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShruthi Kalgudde Gopal,Ruoxuan Dai,Ania Maria Stefanska,Meshal Ansari,Jiakuan Zhao,Pushkar Ramesh,Johannes W Bagnoli,Donovan Correa-Gallegos,Yue Lin,Simon Christ,Ilias Angelidis,Valerio Lupperger,Carsten Marr,Lindsay C Davies,Wolfgang Enard,Hans-Günther Machens,Herbert B Schiller,Dongsheng Jiang,Yuval RinkevichPubMed 37230982
- Stem cell research & therapy 14:672023Human adipose tissue-derived small extracellular vesicles promote soft tissue repair through modulating M1-to-M2 polarization of macrophages.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJia Dong,Bin Wu,Weidong TianPubMed 37024970
- Stem cell reports 18:999-10142023WNT7A suppresses adipogenesis of skeletal muscle mesenchymal stem cells and fatty infiltration through the alternative Wnt-Rho-YAP/TAZ signaling axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChengcheng Fu,Britney Chin-Young,GaYoung Park,Mariana Guzmán-Seda,Damien Laudier,Woojin M HanPubMed 37001514
- Cell death & disease 14:752023SARS-CoV-2 infection induces persistent adipose tissue damage in aged golden Syrian hamsters.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGemma Bogard,Johanna Barthelemy,Aline Hantute-Ghesquier,Valentin Sencio,Patricia Brito-Rodrigues,Karin Séron,Cyril Robil,Anne Flourens,Florence Pinet,Delphine Eberlé,François Trottein,Martine Duterque-Coquillaud,Isabelle WolowczukPubMed 36725844
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.01% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Rat Plin1 aa 500 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P43884
Consider this alternative
Reactivity data
Select an application| ICC | Flow Cyt | WB | IHC-FoFr | IHC-P | |
|---|---|---|---|---|---|
| Human | Predicted | Expected | Predicted | Expected | Expected |
| Mouse | Tested | Expected | Tested | Expected | Expected |
| Rat | Predicted | Expected | Predicted | Expected | Expected |
| Cow | Predicted | Expected | Predicted | Expected | Expected |
| Dog | Predicted | Expected | Predicted | Expected | Expected |
| Rhesus monkey | Predicted | Expected | Predicted | Expected | Expected |
ICC
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 2 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Rhesus monkey, Human, Dog, Rat | Dilution info - | Notes - |
Flow Cyt
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Rhesus monkey, Human, Dog, Cow | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000 - 1/4000 | Notes By Western blot, this antibody detects an ~62 kDa protein representing Perilipin-1 from 3T3-L1 cell extract. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Rhesus monkey, Human, Dog, Rat | Dilution info - | Notes - |
IHC-FoFr
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Rhesus monkey, Human, Dog, Cow | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Rhesus monkey, Human, Dog, Cow | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Modulator of adipocyte lipid metabolism. Coats lipid storage droplets to protect them from breakdown by hormone-sensitive lipase (HSL). Its absence may result in leanness. Plays a role in unilocular lipid droplet formation by activating CIDEC. Their interaction promotes lipid droplet enlargement and directional net neutral lipid transfer. May modulate lipolysis and triglyceride levels (By similarity).
Alternative names
Peri, Plin, Plin1, Perilipin-1, Lipid droplet-associated protein
Recommended products
Anti-perilipin-1 antibody is a rabbit polyclonal antibody that is used in Plin1 western blot (WB), IHC, immunocytochemistry (ICC), and flow cytometry. Suitable for human, mouse, rat, and more samples.
- Cited in over 130 publications
- One antibody for all your perilipin-1 staining
- Trusted by researchers since 2003
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.01% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Rat Plin1 aa 500 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P43884
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Anti-Perilipin-1 antibody (ab3526) is a rabbit polyclonal antibody and is validated for use in Flow Cyt, ICC, IHC-FoFr, IHC-P, WB in cow, dog, human, mouse, rat, rhesus monkey samples.
Anti-Perilipin-1 antibody (ab3526) specifically detects Perilipin-1 (UniProt ID: O60240; Molecular weight: 56kDa) and is sold in 100 µL selling sizes.
Quality and Validation
Abcam's high quality validation processes ensure Anti-Perilipin-1 antibody (ab3526) has high sensitivity and specificity.
Anti-Perilipin-1 antibody (ab3526) has been cited over 135 times in peer reviewed journals and is trusted by the scientific community.
Anti-Perilipin-1 antibody (ab3526) has 15 independent reviews from customers.
Related Products
Conjugation-ready, carrier free format available for antibody clone - Anti-Sp7 / Osterix antibody [EPR21034] - BSA and Azide free ab227820.
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
Immunocytochemistry - Anti-Perilipin-1 antibody (ab3526)
Immunocytochemistry analysis of 3T3-L1 cells (Mouse embryonic fibroblast cell line), 4% paraformaldehyde fixed (10 minutes) permeabilized with 0.1% Triton™ X-100 (10 minutes), and blocked with 1% BSA for (1 hour).
Perilipin A was labeled with ab3526 at 2 μg/ml in 0.1% BSA and incubated for 3 hours at room temperature with secondary antibody: Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate at a dilution of 1:2000 for 45 minutes at room temperature (Panel A: green).
Panel B: Nuclei were stained with DAPI (blue); Panel C: (red) F-actin stained with Alexa Fluor® 555 Rhodamine Phalloidin 1:300. Panel D: merged image showing cytosolic localization; Panel E: untreated; Panel F: control cells with no primary antibody.
Western blot - Anti-Perilipin-1 antibody (ab3526)
Western blot detection of Perilipin-1 in 3T3-L1 cell extract using ab3526.
All lanes: Western blot - Anti-Perilipin-1 antibody (ab3526)
Predicted band size: 56 kDa
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com