AB3526

Anti-Perilipin-1 antibody

Name: Anti-Perilipin-1 antibody. Rabbit polyclonal (ab3526) | Abcam
Brand: Abcam Limited
SKU: AB3526
Price: 505 USD
Availability: InStock
Rating: 4 (16 reviews)

(16 Reviews)

(133 Publications)

Anti-Perilipin-1 antibody (ab3526) is a rabbit polyclonal antibody detecting Perilipin-1 in Western Blot, Flow Cytometry, IHC-P. Suitable for Cow, Dog, Human, Mouse, Rat, Rhesus monkey.

- Over 130 publications
- Trusted since 2003

View Alternative Names

Peri, Plin, Plin1, Perilipin-1, Lipid droplet-associated protein

2 Images

Supplier Data

Immunocytochemistry - Anti-Perilipin-1 antibody (AB3526)

Immunocytochemistry analysis of 3T3-L1 cells (Mouse embryonic fibroblast cell line), 4% paraformaldehyde fixed (10 minutes) permeabilized with 0.1% Triton™ X-100 (10 minutes), and blocked with 1% BSA for (1 hour).

Perilipin A was labeled with ab3526 at 2 μg/ml in 0.1% BSA and incubated for 3 hours at room temperature with secondary antibody : Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate at a dilution of 1 : 2000 for 45 minutes at room temperature (Panel A : green).

Panel B : Nuclei were stained with DAPI (blue); Panel C : (red) F-actin stained with Alexa Fluor® 555 Rhodamine Phalloidin 1 : 300. Panel D : merged image showing cytosolic localization; Panel E : untreated; Panel F : control cells with no primary antibody.

Unknown

Western blot - Anti-Perilipin-1 antibody (AB3526)

Western blot detection of Perilipin-1 in 3T3-L1 cell extract using ab3526.

All lanes:

Western blot - Anti-Perilipin-1 antibody (ab3526)

Predicted band size: 56 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Cow, Rhesus monkey, Human, Dog, Rat

Applications

IHC-FoFr, Flow Cyt, ICC, WB, IHC-P

applications

Immunogen

Synthetic Peptide within Rat Plin1 aa 500 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P43884

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Reactivity data

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Product details

Product Specifications
Anti-Perilipin-1 antibody (ab3526) is a rabbit polyclonal antibody and is validated for use in Flow Cyt, ICC, IHC-FoFr, IHC-P, WB in cow, dog, human, mouse, rat, rhesus monkey samples.
Anti-Perilipin-1 antibody (ab3526) specifically detects Perilipin-1 (UniProt ID: O60240; Molecular weight: 56kDa) and is sold in 100 µL selling sizes.

Quality and Validation
Abcam's high quality validation processes ensure Anti-Perilipin-1 antibody (ab3526) has high sensitivity and specificity.
Anti-Perilipin-1 antibody (ab3526) has been cited over 135 times in peer reviewed journals and is trusted by the scientific community.
Anti-Perilipin-1 antibody (ab3526) has 15 independent reviews from customers.

Related Products
Conjugation-ready, carrier free format available for antibody clone - ab227820.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

Preservative: 0.05% Sodium azide Constituents: 99% PBS, 0.01% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Modulator of adipocyte lipid metabolism. Coats lipid storage droplets to protect them from breakdown by hormone-sensitive lipase (HSL). Its absence may result in leanness. Plays a role in unilocular lipid droplet formation by activating CIDEC. Their interaction promotes lipid droplet enlargement and directional net neutral lipid transfer. May modulate lipolysis and triglyceride levels (By similarity).

See full target information Plin1

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Publications (133)

Recent publications for all applications. Explore the full list and refine your search

Cell death & disease 14:776 PubMed38012162

2023

Deubiquitinase USP1 enhances CCAAT/enhancer-binding protein beta (C/EBPβ) stability and accelerates adipogenesis and lipid accumulation.

Applications

Unspecified application

Species

Unspecified reactive species

Myung Sup Kim,Jung-Hwan Baek,JinAh Lee,Aneesh Sivaraman,Kyeong Lee,Kyung-Hee Chun

Journal of animal science and biotechnology 14:141 PubMed37919760

2023

Single-base editing in IGF2 improves meat production and intramuscular fat deposition in Liang Guang Small Spotted pigs.

Applications

Unspecified application

Species

Unspecified reactive species

Tianqi Duo,Xiaohong Liu,Delin Mo,Yu Bian,Shufang Cai,Min Wang,Ruiqiang Li,Qi Zhu,Xian Tong,Ziyun Liang,Weilun Jiang,Shiyi Chen,Yaosheng Chen,Zuyong He

Biotechnology journal 18:e2300098 PubMed37449520

2023

Effect of radiation sterilization on the ability to induce adipose regeneration in vivo in decellularized adipose-derived matrix.

Applications

Unspecified application

Species

Unspecified reactive species

Jiayi Feng,Jun Qi,Su Fu,Jie Luan

Experimental & molecular medicine 55:1520-1530 PubMed37394587

2023

Ablation of the deubiquitinase USP15 ameliorates nonalcoholic fatty liver disease and nonalcoholic steatohepatitis.

Applications

Unspecified application

Species

Unspecified reactive species

Jung-Hwan Baek,Myung Sup Kim,Hye Ryeon Jung,Min-Seon Hwang,Chan-Ho Lee,Dai Hoon Han,Yong-Ho Lee,Eugene C Yi,Seung-Soon Im,Ilseon Hwang,Kyungeun Kim,Joon-Yong Chung,Kyung-Hee Chun

Bioengineering (Basel, Switzerland) 10: PubMed37508785

2023

Selection of Mechanical Fragmentation Methods Based on Enzyme-Free Preparation of Decellularized Adipose-Derived Matrix.

Applications

Unspecified application

Species

Unspecified reactive species

Jiayi Feng,Su Fu,Jie Luan

International journal of nanomedicine 18:2923-2938 PubMed37288352

2023

Preparation of Apoptotic Extracellular Vesicles from Adipose Tissue and Their Efficacy in Promoting High-Quality Skin Wound Healing.

Applications

Unspecified application

Species

Unspecified reactive species

Jia Dong,Bin Wu,Weidong Tian

Nature communications 14:3020 PubMed37230982

2023

Wound infiltrating adipocytes are not myofibroblasts.

Applications

Unspecified application

Species

Unspecified reactive species

Shruthi Kalgudde Gopal,Ruoxuan Dai,Ania Maria Stefanska,Meshal Ansari,Jiakuan Zhao,Pushkar Ramesh,Johannes W Bagnoli,Donovan Correa-Gallegos,Yue Lin,Simon Christ,Ilias Angelidis,Valerio Lupperger,Carsten Marr,Lindsay C Davies,Wolfgang Enard,Hans-Günther Machens,Herbert B Schiller,Dongsheng Jiang,Yuval Rinkevich

Stem cell research & therapy 14:67 PubMed37024970

2023

Human adipose tissue-derived small extracellular vesicles promote soft tissue repair through modulating M1-to-M2 polarization of macrophages.

Applications

Unspecified application

Species

Unspecified reactive species

Jia Dong,Bin Wu,Weidong Tian

Stem cell reports 18:999-1014 PubMed37001514

2023

WNT7A suppresses adipogenesis of skeletal muscle mesenchymal stem cells and fatty infiltration through the alternative Wnt-Rho-YAP/TAZ signaling axis.

Applications

Unspecified application

Species

Unspecified reactive species

Chengcheng Fu,Britney Chin-Young,GaYoung Park,Mariana Guzmán-Seda,Damien Laudier,Woojin M Han

Cell death & disease 14:75 PubMed36725844

2023

SARS-CoV-2 infection induces persistent adipose tissue damage in aged golden Syrian hamsters.

Applications

Unspecified application

Species

Unspecified reactive species

Gemma Bogard,Johanna Barthelemy,Aline Hantute-Ghesquier,Valentin Sencio,Patricia Brito-Rodrigues,Karin Séron,Cyril Robil,Anne Flourens,Florence Pinet,Delphine Eberlé,François Trottein,Martine Duterque-Coquillaud,Isabelle Wolowczuk

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

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Associated Products

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