Anti-Perilipin-1 antibody [EPR28189-12] - BSA and Azide free

• mIHC

Lab

Multiplex immunohistochemistry - Anti-Perilipin-1 antibody [EPR28189-12] - BSA and Azide free (AB316116)

This data was developed using ab316115, the same antibody clone in a different buffer formulation.

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded mouse breast tissue staining CD34 with ab316277 at a 1 : 10000 (0.05 ug/ml) dilution, ab324191 E Cadherin used at 1 : 500 (0.228 ug/ml) dilution and ab316115 Perilipin-1 used at 1 : 2000 (0.251 μg/ml) dilution.

Panel A : merged staining of anti-CD34 (green; Opal™570), anti-E Cadherin (magenta; Opal™690) and anti-Perilipin-1 (gray; Opal™570) on mouse breast.

Panel B : anti-CD34 staining endothelium in mouse breast.

Panel C : ant-E Cadherin staining epithelium in mouse breast.

Panel D : ant-Perilipin-1 staining adipocytes in mouse breast.

Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab316277, ab324191 and ab316115 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Perilipin-1 antibody [EPR28189-12] - BSA and Azide free (AB316116)

This data was developed using ab316115, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse skeletal muscle tissue labeling Perilipin-1 with ab316115 at 1/2000 (0.251 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Negative control : No staining on mouse skeletal muscle.
The section was incubated with ab316115 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Perilipin-1 antibody [EPR28189-12] - BSA and Azide free (AB316116)

This data was developed using ab316115, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling Perilipin-1 with ab316115 at 1/2000 (0.251 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Negative control : No staining on mouse kidney.
The section was incubated with ab316115 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Perilipin-1 antibody [EPR28189-12] - BSA and Azide free (AB316116)

This data was developed using ab316115, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse adipose tissue tissue labeling Perilipin-1 with ab316115 at 1/2000 (0.251 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on mouse adipose tissue (PMID : 25289234).
The section was incubated with ab316115 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Perilipin-1 antibody [EPR28189-12] - BSA and Azide free (AB316116)

This data was developed using ab316115, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse testis tissue labeling Perilipin-1 with ab316115 at 1/2000 (0.251 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on Leydig cells of mouse testis (PMID : 32580404).
The section was incubated with ab316115 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Perilipin-1 antibody [EPR28189-12] - BSA and Azide free (AB316116)

This data was developed using ab316115, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 3T3-L1 (mouse embryonic fibroblast) cells labelling Perilipin-1 with ab316115 at 1/500 (1.004 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/mL) dilution (Green).

Confocal image showing cytoplasmic staining in differentiated 3T3-L1 cells.
Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).

ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (2.5 ug/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution.

• mIHC

Lab

Multiplex immunohistochemistry - Anti-Perilipin-1 antibody [EPR28189-12] - BSA and Azide free (AB316116)

This data was developed using ab316115, the same antibody clone in a different buffer formulation.

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded mouse breast tissue staining Heparan Sulfate Proteoglycan 2/Perlecan with ab315029 at a 1/100 dilution, ab324191 anti-E Cadherin used at 1/500 dilution and ab316115 anti-Perilipin-1 used at a 1/2000 dilution.

Panel A : merged staining of anti-Perlecan (green; Opal™520), anti-E Cadherin (magenta; Opal™690) and anti-Perilipin-1 (gray; Opal™570) on mouse breast.

Panel B : anti-Perlecan staining endothelium and basement membrane in mouse breast.

Panel C : anti-E Cadherin staining epithelium in mouse breast.

Panel D : anti-Perilipin-1 staining adipocytes in mouse breast.

Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab325029, ab324191 and ab316115 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IP

Supplier Data

Immunoprecipitation - Anti-Perilipin-1 antibody [EPR28189-12] - BSA and Azide free (AB316116)

This data was developed using ab316115, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Perilipin-1 was immunoprecipitated from 0.35 mg 3T3-L1 (mouse embryonic fibroblast) differentiation for 6 days whole cell lysate with ab316115 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab316115 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : 3T3-L1 (mouse embryonic fibroblast) differentiation for 6 days whole cell lysate
Lane 2 : ab316115 IP in 3T3-L1 (mouse embryonic fibroblast) differentiation for 6 days whole cell lysate
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab316115 in 3T3-L1 differentiation for 6 days whole cell lysate.

All lanes:

Immunoprecipitation - Anti-Perilipin-1 antibody [EPR28189-12] (<a href='/en-us/products/primary-antibodies/perilipin-1-antibody-epr28189-12-ab316115'>ab316115</a>) at 1/30 dilution

All lanes:

3T3-L1 (mouse embryonic fibroblast) differentiation for 6 days whole cell lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

false

Exposure time: 137s