Rabbit Recombinant Monoclonal Perilipin-1 antibody. Carrier free. Suitable for WB, IP, ICC/IF and reacts with Mouse samples.
Images
![Western blot - Anti-Perilipin-1 antibody [EPR28189-66] - BSA and Azide free (AB317261), expandable thumbnail](https://content.abcam.com/products/images/perilipin-1-antibody-epr28189-66-bsa-and-azide-free-ab317261--western-blot-img429611.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Perilipin-1 antibody [EPR28189-66] - BSA and Azide free (AB317261)")
![Western blot - Anti-Perilipin-1 antibody [EPR28189-66] - BSA and Azide free (AB317261), expandable thumbnail](https://content.abcam.com/products/images/perilipin-1-antibody-epr28189-66-bsa-and-azide-free-ab317261--western-blot-img429612.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Perilipin-1 antibody [EPR28189-66] - BSA and Azide free (AB317261)")
![Immunocytochemistry/ Immunofluorescence - Anti-Perilipin-1 antibody [EPR28189-66] - BSA and Azide free (AB317261), expandable thumbnail](https://content.abcam.com/products/images/perilipin-1-antibody-epr28189-66-bsa-and-azide-free-ab317261--immunocytochemistry-immunofluorescence-img429698.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Perilipin-1 antibody [EPR28189-66] - BSA and Azide free (AB317261)")
![Immunoprecipitation - Anti-Perilipin-1 antibody [EPR28189-66] - BSA and Azide free (AB317261), expandable thumbnail](https://content.abcam.com/products/images/perilipin-1-antibody-epr28189-66-bsa-and-azide-free-ab317261--immunoprecipitation-img429624.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-Perilipin-1 antibody [EPR28189-66] - BSA and Azide free (AB317261)")
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | IP | ICC/IF | Flow Cyt (Intra) | IHC-P | |
|---|---|---|---|---|---|
| Human | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Mouse | Tested | Tested | Tested | Not recommended | Not recommended |
| Rat | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Rat | Dilution info - | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Rat | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Rat | Dilution info - | Notes - |
Flow Cyt (Intra)
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human, Rat | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Target data
Function
Modulator of adipocyte lipid metabolism. Coats lipid storage droplets to protect them from breakdown by hormone-sensitive lipase (HSL). Its absence may result in leanness (By similarity). Plays a role in unilocular lipid droplet formation by activating CIDEC. Their interaction promotes lipid droplet enlargement and directional net neutral lipid transfer. May modulate lipolysis and triglyceride levels.
Alternative names
Perilipin-1, Lipid droplet-associated protein, Perilipin A, Cnlp, Camp, Cramp
Recommended products
Rabbit Recombinant Monoclonal Perilipin-1 antibody. Carrier free. Suitable for WB, IP, ICC/IF and reacts with Mouse samples.
Alternative names
Perilipin-1, Lipid droplet-associated protein, Perilipin A, Cnlp, Camp, Cramp
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
Yes
- Clone number
EPR28189-66
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
Notes
ab317261 is the carrirer-free version of Anti-Perilipin-1 antibody [EPR28189-66] ab317260.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Perilipin-1 also known as PLIN1 is a protein with a mass of approximately 57 kDa. It plays an important role in lipid metabolism within adipose tissue. This protein is mainly found in adipocytes where it coats lipid droplets. By controlling the access of lipases to the lipid core Perilipin-1 regulates lipolysis. It prevents unnecessary breakdown of lipids by acting as a barrier and ensuring the protection and storage of energy reserves. This targeted regulation is important for maintaining energy homeostasis in the body.
- Biological function summary
Perilipin-1 influences cellular energy balance and fat storage. It is an important member of the PAT family of proteins which includes perilipin A. Perilipin-1 does not typically form part of a larger protein complex; however it works closely with other proteins on the surface of lipid droplets. Its presence on the droplet surface is essential for controlling lipid mobilization particularly during fasting or energy-deprived states when organisms require a mechanism to access stored energy efficiently.
- Pathways
The activity of Perilipin-1 is important in the lipolytic pathway. This pathway is involved in hydrolyzing stored triglycerides into free fatty acids and glycerol an essential process in energy metabolism. Perilipin-1 acts in tandem with hormone-sensitive lipase (HSL) to regulate this pathway. Upon activation by adrenaline or other stimuli perilipin-1 undergoes phosphorylation which in turn facilitates HSL translocation to the lipid droplet and enhances lipolysis. Besides its direct role Perilipin-1 also interacts with adipose triglyceride lipase (ATGL) further impacting lipid breakdown processes.
- Associated diseases and disorders
The dysfunction of Perilipin-1 can lead to metabolic diseases like obesity and type 2 diabetes. Abnormal regulation or mutations in the Perilipin-1 gene have been observed in these conditions affecting normal lipid metabolism. Disordered Perilipin-1 function can lead to excessive lipid storage impaired energy mobilization and increased insulin resistance. Additionally Perilipin-1 interacts indirectly with other proteins such as perilipin-2 which can also influence the development of these metabolic disorders. Understanding these interactions helps in exploring therapeutic strategies aimed at targeting lipid metabolism and related diseases.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Western blot - Anti-Perilipin-1 antibody [EPR28189-66] - BSA and Azide free (ab317261), expandable thumbnail](https://content.abcam.com/products/images/perilipin-1-antibody-epr28189-66-bsa-and-azide-free-ab317261--western-blot-img429611.jpg "Western blot - Anti-Perilipin-1 antibody [EPR28189-66] - BSA and Azide free (ab317261)")
Western blot - Anti-Perilipin-1 antibody [EPR28189-66] - BSA and Azide free (ab317261)
This data was developed using Anti-Perilipin-1 antibody [EPR28189-66] ab317260, the same antibody clone in a different buffer formulation.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
All lanes: Western blot - Anti-Perilipin-1 antibody [EPR28189-66] (Anti-Perilipin-1 antibody [EPR28189-66] ab317260) at 1/1000 dilution
Lane 1: Undifferentiated 3T3-L1 (mouse embryonic fibroblast) whole cell lysate at 20 µg
Lane 2: Differentiated 3T3-L1 for 6 days whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 55 kDa, 36 kDa
Exposure time: 180s
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
![Western blot - Anti-Perilipin-1 antibody [EPR28189-66] - BSA and Azide free (ab317261), expandable thumbnail](https://content.abcam.com/products/images/perilipin-1-antibody-epr28189-66-bsa-and-azide-free-ab317261--western-blot-img429612.jpg "Western blot - Anti-Perilipin-1 antibody [EPR28189-66] - BSA and Azide free (ab317261)")
Western blot - Anti-Perilipin-1 antibody [EPR28189-66] - BSA and Azide free (ab317261)
This data was developed using Anti-Perilipin-1 antibody [EPR28189-66] ab317260, the same antibody clone in a different buffer formulation.
Low expression: mouse skeletal muscle (PMID: 32996002).
Lysates were freshly made and used for Western blotting immediately to minimize protein degradation.
The bands beneath the target band (55 kDa) are likely to be degraded target fragments (PMID: 16448845).
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
Exposure time: Lane 1-2: 37 seconds; Lane 3: 180 seconds
All lanes: Western blot - Anti-Perilipin-1 antibody [EPR28189-66] (Anti-Perilipin-1 antibody [EPR28189-66] ab317260) at 1/1000 dilution
Lane 1: Mouse white fat tissue lysate at 20 µg
Lane 2: Mouse brown fat tissue lysate at 20 µg
Lane 3: Mouse skeletal muscle tissue lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 55 kDa, 36 kDa
Low expression: mouse skeletal muscle (PMID: 32996002).
Lysates were freshly made and used for Western blotting immediately to minimize protein degradation.
The bands beneath the target band (55 kDa) are likely to be degraded target fragments (PMID: 16448845).
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
Exposure time: Lane 1-2: 37 seconds; Lane 3: 180 seconds
![Immunocytochemistry/ Immunofluorescence - Anti-Perilipin-1 antibody [EPR28189-66] - BSA and Azide free (ab317261), expandable thumbnail](https://content.abcam.com/products/images/perilipin-1-antibody-epr28189-66-bsa-and-azide-free-ab317261--immunocytochemistry-immunofluorescence-img429698.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Perilipin-1 antibody [EPR28189-66] - BSA and Azide free (ab317261)")
Immunocytochemistry/ Immunofluorescence - Anti-Perilipin-1 antibody [EPR28189-66] - BSA and Azide free (ab317261)
This data was developed using Anti-Perilipin-1 antibody [EPR28189-66] ab317260, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 3T3-L1 (mouse embryonic fibroblast) cells labelling Perilipin-1 with Anti-Perilipin-1 antibody [EPR28189-66] ab317260 at 1/1000 (0.493 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green).
Confocal image showing cytoplasmic staining in differentiated 3T3-L1 cells (shown in green). The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue).
Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (2.5ug/ml) dilution (Magenta). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/ml) dilution.
![Immunoprecipitation - Anti-Perilipin-1 antibody [EPR28189-66] - BSA and Azide free (ab317261), expandable thumbnail](https://content.abcam.com/products/images/perilipin-1-antibody-epr28189-66-bsa-and-azide-free-ab317261--immunoprecipitation-img429624.jpg "Immunoprecipitation - Anti-Perilipin-1 antibody [EPR28189-66] - BSA and Azide free (ab317261)")
Immunoprecipitation - Anti-Perilipin-1 antibody [EPR28189-66] - BSA and Azide free (ab317261)
This data was developed using Anti-Perilipin-1 antibody [EPR28189-66] ab317260, the same antibody clone in a different buffer formulation.
Perilipin-1 was immunoprecipitated from 0.35 mg Differentiated 3T3-L1(mouse embryonic fibroblast) for 6 days whole cell lysate with Anti-Perilipin-1 antibody [EPR28189-66] ab317260 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-Perilipin-1 antibody [EPR28189-66] ab317260 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: Differentiated 3T3-L1(mouse embryonic fibroblast) for 6 days whole cell lysate
Lane 2: Anti-Perilipin-1 antibody [EPR28189-66] ab317260 IP in Differentiated 3T3-L1(mouse embryonic fibroblast) for 6 days whole cell lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-Perilipin-1 antibody [EPR28189-66] ab317260 in differentiated 3T3-L1 for 6 days whole cell lysate.The bands beneath the target band (55 kDa) are likely to be degraded target fragments (PMID: 16448845).
All lanes: Immunoprecipitation - Anti-Perilipin-1 antibody [EPR28189-66] (Anti-Perilipin-1 antibody [EPR28189-66] ab317260) at 1/30 dilution
All lanes: Differentiated 3T3-L1(mouse embryonic fibroblast) for 6 days whole cell lysate
SecondaryAll lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution
Exposure time: 10s
Perilipin-1 was immunoprecipitated from 0.35 mg Differentiated 3T3-L1(mouse embryonic fibroblast) for 6 days whole cell lysate with Anti-Perilipin-1 antibody [EPR28189-66] ab317260 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-Perilipin-1 antibody [EPR28189-66] ab317260 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: Differentiated 3T3-L1(mouse embryonic fibroblast) for 6 days whole cell lysate
Lane 2: Anti-Perilipin-1 antibody [EPR28189-66] ab317260 IP in Differentiated 3T3-L1(mouse embryonic fibroblast) for 6 days whole cell lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-Perilipin-1 antibody [EPR28189-66] ab317260 in differentiated 3T3-L1 for 6 days whole cell lysate.The bands beneath the target band (55 kDa) are likely to be degraded target fragments (PMID: 16448845).
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