Rabbit Polyclonal Perilipin-2 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 15 publications.
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- Developmental dynamics : an official publication of the American Association of Anatomists 252:536-5462023Characterization of a novel Hoxa5eGFP mouse line.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMu-Hang Li,Julia M Kuetemeyer,Alisha R Yallowitz,Deneen M WellikPubMed 36577717
- Cell reports 39:1107962022Lipogenesis mediated by OGR1 regulates metabolic adaptation to acid stress in cancer cells via autophagy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSmitha Pillai,Iqbal Mahmud,Rohit Mahar,Crystal Griffith,Michael Langsen,Jonathan Nguyen,Jonathan W Wojtkowiak,Pawel Swietach,Robert A Gatenby,Marilyn M Bui,Matthew E Merritt,Patricia McDonald,Timothy J Garrett,Robert J GilliesPubMed 35545051
- Cancer research 82:1267-12822022Lactate Rewires Lipid Metabolism and Sustains a Metabolic-Epigenetic Axis in Prostate Cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLuigi Ippolito,Giuseppina Comito,Matteo Parri,Marta Iozzo,Assia Duatti,Francesca Virgilio,Nicla Lorito,Marina Bacci,Elisa Pardella,Giada Sandrini,Francesca Bianchini,Roberta Damiano,Lavinia Ferrone,Giancarlo la Marca,Sergio Serni,Pietro Spatafora,Carlo V Catapano,Andrea Morandi,Elisa Giannoni,Paola ChiarugiPubMed 35135811
- International journal of molecular sciences 22:2021Leptin Reduces Plin5 mA Methylation through FTO to Regulate Lipolysis in Piglets.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDongqin Wei,Qian Sun,Yizhou Li,Chaowei Li,Xinjian Li,Chao SunPubMed 34638947
- Journal of cell science 135:2021Co-translational biogenesis of lipid droplet integral membrane proteins.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPawel Leznicki,Hayden O Schneider,Jada V Harvey,Wei Q Shi,Stephen HighPubMed 34558621
- The Journal of clinical investigation 131:2021WNT6/ACC2-induced storage of triacylglycerols in macrophages is exploited by Mycobacterium tuberculosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJulius Brandenburg,Sebastian Marwitz,Simone C Tazoll,Franziska Waldow,Barbara Kalsdorf,Tim Vierbuchen,Thomas Scholzen,Annette Gross,Svenja Goldenbaum,Alexandra Hölscher,Martina Hein,Lara Linnemann,Maja Reimann,Andreas Kispert,Michael Leitges,Jan Rupp,Christoph Lange,Stefan Niemann,Jochen Behrends,Torsten Goldmann,Holger Heine,Ulrich E Schaible,Christoph Hölscher,Dominik Schwudke,Norbert ReilingPubMed 34255743
- Cardiovascular research 117:547-5602020Class A1 scavenger receptor prevents obesity-associated blood pressure elevation through suppressing overproduction of vascular endothelial growth factor B in macrophages.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXudong Zhu,Yan Wang,Liu Zhu,Ye Zhu,Kun Zhang,Lei Wang,Hui Bai,Qing Yang,Jingjing Ben,Hanwen Zhang,Xiaoyu Li,Yong Xu,Qi ChenPubMed 32044963
- eLife 8:2019Human VPS13A is associated with multiple organelles and influences mitochondrial morphology and lipid droplet motility.Applications:WBReactive species:Unspecified reactive speciesWondwossen M Yeshaw et. al.PubMed 30741634
- Reproduction, fertility, and development 31:774-7862018Lipophagy contributes to long-term storage of spermatozoa in the epididymis of the Chinese soft-shelled turtle Pelodiscus sinensis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHong Chen,Yufei Huang,Ping Yang,Tengfei Liu,Nisar Ahmed,Lingling Wang,Taozhi Wang,Xuebing Bai,Abdul Haseeb,Qiusheng ChenPubMed 30526797
- The Journal of biological chemistry 294:379-3882018N-terminal acetylation and the N-end rule pathway control degradation of the lipid droplet protein PLIN2.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKha The Nguyen,Chang-Seok Lee,Sang-Hyeon Mun,Nhung Thimy Truong,Sang Ki Park,Cheol-Sang HwangPubMed 30425097
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Consider this alternative
Reactivity data
Select an application| WB | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
| Orangutan | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Orangutan | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 5 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Orangutan | Dilution info - | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
Structural component of lipid droplets, which is required for the formation and maintenance of lipid storage droplets.
Alternative names
ADFP, PLIN2, Perilipin-2, Adipophilin, Adipose differentiation-related protein, ADRP
Recommended products
Rabbit Polyclonal Perilipin-2 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 15 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The protein Perilipin-2 also known as PLIN2 ADFP or adipophilin has an approximate molecular weight of 48 kDa. It is primarily expressed in tissues involved in lipid metabolism like adipose tissue liver and muscle. The main role of Perilipin-2 is to coat lipid droplets in cells thereby regulating lipid storage and breakdown. This protein serves as a critical component of the lipid droplet surface providing structure and protecting the lipid core from excessive unregulated lipolysis.
- Biological function summary
Perilipin-2 is a member of the PAT family proteins involved in lipid droplet formation and maintenance. It does not typically form part of larger complexes but it plays a significant role in cellular energy regulation by controlling lipid reserves. This regulation ensures an efficient balance between energy storage and mobilization. The presence and levels of Perilipin-2 can influence how cells respond to nutrients particularly during periods of energy surplus or fasting.
- Pathways
Perilipin-2 is heavily involved in the regulation of lipid metabolic pathways and the storage-related signaling cascades. It interacts with key metabolic proteins including hormone-sensitive lipase (HSL) to modulate lipolysis. By doing so Perilipin-2 influences processes like fatty acid mobilization and glycerol release integrating perfectly into the cellular energy balance system. The protein is also related to perilipin another member of the lipid droplet-associated proteins emphasizing its participation in lipid metabolism.
- Associated diseases and disorders
Altered expression of Perilipin-2 can have implications in obesity and fatty liver disease. The protein is involved in the accumulation of lipid droplets which is a common feature in these conditions. Obesity and associated disorders often show elevated levels of Perilipin-2 which mirrors increased lipid storage capacity. The protein’s interplay with adipose tissue composition highlights its connection to obesity-related factors including other lipid droplet-associated proteins like adipose triglyceride lipase (ATGL) which influence the progression of metabolic diseases.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Western blot - Anti-Perilipin 2 antibody (ab78920)
All lanes: Western blot - Anti-Perilipin 2 antibody (ab78920) at 1 µg/mL
Lane 1: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
Lane 2: Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate at 10 µg
Lane 3: HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg
Lane 4: HEK293 (Human embryonic kidney cell line) Whole Cell Lysate at 10 µg
SecondaryAll lanes: Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 48 kDa
Observed band size: 25 kDa, 48 kDa
Exposure time: 20min
Immunocytochemistry/ Immunofluorescence - Anti-Perilipin 2 antibody (ab78920)
ab78920 staining Perilipin-2 in HepG2 cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab78920 at 5μg/ml and Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).
Also suitable in cells fixed with 100% methanol (5 min).
Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.
Western blot - Anti-Perilipin 2 antibody (ab78920)
All lanes: Western blot - Anti-Perilipin 2 antibody (ab78920) at 1 µg/mL
All lanes: Recombinant Human ADFP protein (ab73631) at 0.1 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 48 kDa
Exposure time: 1min
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