Mouse Monoclonal Perilipin-2 antibody. Suitable for IHC-P and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human PLIN2 aa 200-400.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Mouse monoclonal [ADFP/1494] to Perilipin-2 (AB219301), expandable thumbnail](https://content.abcam.com/products/images/perilipin-2-antibody-adfp-1494-ab219301--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img105426.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Mouse monoclonal [ADFP/1494] to Perilipin-2 (AB219301)")
Key facts
- Isotype
IgG2b
- Host species
Mouse
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.05% BSA- Form
Liquid
- Clonality
Monoclonal
Immunogen
- Recombinant Fragment Protein within Human PLIN2 aa 200-400. The exact immunogen used to generate this antibody is proprietary information. Database link Q99541
Reactivity data
Select an application| IHC-P | |
|---|---|
| Human | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 2-4 µg/mL | Notes (Primary incubation for 30 minutes at RT). Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Associated Products
Select an associated product type
1 products for Alternative Version
3 products for Alternative Product
Target data
Function
May be involved in development and maintenance of adipose tissue.
Alternative names
Perilipin-2, Adipophilin, Adipose differentiation-related protein, ADRP, ADFP, PLIN2
Recommended products
Mouse Monoclonal Perilipin-2 antibody. Suitable for IHC-P and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human PLIN2 aa 200-400.
Alternative names
Perilipin-2, Adipophilin, Adipose differentiation-related protein, ADRP, ADFP, PLIN2
Key facts
- Isotype
IgG2b
- Host species
Mouse
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.05% BSA- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- Recombinant Fragment Protein within Human PLIN2 aa 200-400. The exact immunogen used to generate this antibody is proprietary information. Database link Q99541
- Clone number
ADFP/1494
- Purification technique
Affinity purification Protein A/G
- Light chain type
kappa
- Concentration
- Purification notes
ab219301 was purified from Bioreactor Concentrate by Protein A/G.
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The protein Perilipin-2 also known as PLIN2 ADFP or adipophilin has an approximate molecular weight of 48 kDa. It is primarily expressed in tissues involved in lipid metabolism like adipose tissue liver and muscle. The main role of Perilipin-2 is to coat lipid droplets in cells thereby regulating lipid storage and breakdown. This protein serves as a critical component of the lipid droplet surface providing structure and protecting the lipid core from excessive unregulated lipolysis.
- Biological function summary
Perilipin-2 is a member of the PAT family proteins involved in lipid droplet formation and maintenance. It does not typically form part of larger complexes but it plays a significant role in cellular energy regulation by controlling lipid reserves. This regulation ensures an efficient balance between energy storage and mobilization. The presence and levels of Perilipin-2 can influence how cells respond to nutrients particularly during periods of energy surplus or fasting.
- Pathways
Perilipin-2 is heavily involved in the regulation of lipid metabolic pathways and the storage-related signaling cascades. It interacts with key metabolic proteins including hormone-sensitive lipase (HSL) to modulate lipolysis. By doing so Perilipin-2 influences processes like fatty acid mobilization and glycerol release integrating perfectly into the cellular energy balance system. The protein is also related to perilipin another member of the lipid droplet-associated proteins emphasizing its participation in lipid metabolism.
- Associated diseases and disorders
Altered expression of Perilipin-2 can have implications in obesity and fatty liver disease. The protein is involved in the accumulation of lipid droplets which is a common feature in these conditions. Obesity and associated disorders often show elevated levels of Perilipin-2 which mirrors increased lipid storage capacity. The protein’s interplay with adipose tissue composition highlights its connection to obesity-related factors including other lipid droplet-associated proteins like adipose triglyceride lipase (ATGL) which influence the progression of metabolic diseases.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Mouse monoclonal [ADFP/1494] to Perilipin-2 (ab219301), expandable thumbnail](https://content.abcam.com/products/images/perilipin-2-antibody-adfp-1494-ab219301--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img105426.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Mouse monoclonal [ADFP/1494] to Perilipin-2 (ab219301)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Mouse monoclonal [ADFP/1494] to Perilipin-2 (ab219301)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human adrenal tissue labeling Perilipin-2 with ab219301 at 4 μg/ml.
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