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AB270306

Anti-Perilipin-2 antibody [ADFP/2755R] - BSA and Azide free

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Rabbit Recombinant Monoclonal Perilipin-2 antibody. Carrier free. Suitable for WB, IHC-P and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human PLIN2 aa 200-400.

View Alternative Names

ADFP, PLIN2, Perilipin-2, Adipophilin, Adipose differentiation-related protein, ADRP

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Perilipin-2 antibody [ADFP/2755R] - BSA and Azide free (AB270306)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Perilipin-2 antibody [ADFP/2755R] - BSA and Azide free (AB270306)

Fomalin-fixed, parafin-embedded human adrenal gland tissue stained for Perilipin-2 using ab270274 at 2μg/ml in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (ab270274).

Western blot - Anti-Perilipin-2 antibody [ADFP/2755R] - BSA and Azide free (AB270306)
  • WB

Supplier Data

Western blot - Anti-Perilipin-2 antibody [ADFP/2755R] - BSA and Azide free (AB270306)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (ab270274).

All lanes:

Western blot - Anti-Perilipin-2 antibody [ADFP/2755R] - BSA and Azide free (ab270306) at 2 µg/mL

All lanes:

Human adrenal gland tissue

Predicted band size: 48 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

ADFP/2755R

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human PLIN2 aa 200-400. The exact immunogen used to generate this antibody is proprietary information.

Q99541

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1-2 µg/mL", "IHCP-species-notes": "<p></p>" } } }
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Product details

ab270306 is a carrier free version of ab270274.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A/G
Purification notes
Purified from bioreactor concentrate.
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein Perilipin-2 also known as PLIN2 ADFP or adipophilin has an approximate molecular weight of 48 kDa. It is primarily expressed in tissues involved in lipid metabolism like adipose tissue liver and muscle. The main role of Perilipin-2 is to coat lipid droplets in cells thereby regulating lipid storage and breakdown. This protein serves as a critical component of the lipid droplet surface providing structure and protecting the lipid core from excessive unregulated lipolysis.
Biological function summary

Perilipin-2 is a member of the PAT family proteins involved in lipid droplet formation and maintenance. It does not typically form part of larger complexes but it plays a significant role in cellular energy regulation by controlling lipid reserves. This regulation ensures an efficient balance between energy storage and mobilization. The presence and levels of Perilipin-2 can influence how cells respond to nutrients particularly during periods of energy surplus or fasting.

Pathways

Perilipin-2 is heavily involved in the regulation of lipid metabolic pathways and the storage-related signaling cascades. It interacts with key metabolic proteins including hormone-sensitive lipase (HSL) to modulate lipolysis. By doing so Perilipin-2 influences processes like fatty acid mobilization and glycerol release integrating perfectly into the cellular energy balance system. The protein is also related to perilipin another member of the lipid droplet-associated proteins emphasizing its participation in lipid metabolism.

Altered expression of Perilipin-2 can have implications in obesity and fatty liver disease. The protein is involved in the accumulation of lipid droplets which is a common feature in these conditions. Obesity and associated disorders often show elevated levels of Perilipin-2 which mirrors increased lipid storage capacity. The protein’s interplay with adipose tissue composition highlights its connection to obesity-related factors including other lipid droplet-associated proteins like adipose triglyceride lipase (ATGL) which influence the progression of metabolic diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Structural component of lipid droplets, which is required for the formation and maintenance of lipid storage droplets.
See full target information PLIN2
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Product promise

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