AB118605

Anti-Perilipin 3/TIP47 antibody

Name: Anti-Perilipin 3/TIP47 antibody (ab118605) | Abcam
Brand: Abcam Limited
SKU: AB118605
Price: 630 USD
Availability: InStock
Rating: 4 (2 reviews)

(2 Reviews)

(4 Publications)

Goat Polyclonal Perilipin 3/TIP47 antibody. Suitable for IHC-P, WB and reacts with Mouse, Rat samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Mouse Plin3 aa 300-350.

View Alternative Names

M6prbp1, Tip47, Plin3, Perilipin-3, Cargo selection protein TIP47, Mannose-6-phosphate receptor-binding protein 1

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Perilipin 3/TIP47 antibody (AB118605)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Perilipin 3/TIP47 antibody (AB118605)

ab118605 at 5 μg/ml staining Perilipin 3/TIP47 in Mouse Spleen by Immunohistochemistry Formalin-fixed, Paraffin-embedded tissue.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Perilipin 3/TIP47 antibody (AB118605)

ab118605 at 5 μg/ml staining Perilipin 3/TIP47 in Mouse Testis by Immunohistochemistry Formalin-fixed, Paraffin-embedded tissue.

Unknown

Western blot - Anti-Perilipin 3/TIP47 antibody (AB118605)

All lanes:

Western blot - Anti-Perilipin 3/TIP47 antibody (ab118605) at 0.1 µg/mL

All lanes:

Rat Skin lysate in RIPA buffer at 35 µg

Predicted band size: 47 kDa

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Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat

Applications

IHC-P, WB

applications

Immunogen

Synthetic Peptide within Mouse Plin3 aa 300-350. The exact immunogen used to generate this antibody is proprietary information.

Q9DBG5

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-3 µg/mL", "WB-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: 99% Tris buffered saline, 0.5% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Perilipin 3 also known as TIP47 is a lipid droplet-associated protein with a molecular weight of approximately 47 kDa. This protein expresses in a variety of tissues especially those involved in lipid metabolism like adipose tissue and liver. Perilipin 3 localizes on the surface of lipid droplets where it plays an important role in managing lipid storage and mobilization. This protein interacts directly with the lipid droplet surface facilitating stabilization and regulation of lipid content.

Biological function summary

Perilipin 3 acts as a mediator in lipid droplet dynamics and is part of the perilipin family of proteins. These proteins are key modulators of lipid storage and release ensuring balance within lipid metabolism. Perilipin 3 does not form a complex but has functional interactions with other perilipin proteins like perilipin 1 and perilipin 2 which synergize in the regulation of lipid droplets. This interaction ensures the efficiency of lipid metabolism processes and energy homeostasis in cells.

Pathways

Perilipin 3 participates in lipid metabolism and energy homeostasis pathways. It works alongside proteins such as adipose triglyceride lipase (ATGL) and hormone-sensitive lipase (HSL) to manage the lipolytic process. This involves the breakdown of stored triglycerides into free fatty acids and glycerol. The protein is integral in the regulation of lipid storage and catabolism pathways facilitating energy release during increased energy demand conditions.

Perilipin 3 has connections to metabolic diseases such as obesity and lipid storage disorders. Aberrant function or expression of perilipin 3 can lead to disrupted lipid metabolism and contribute to these conditions. Research shows that perilipin 3 works with proteins like ATGL in these contexts as alterations in their activities affect lipid handling. Understanding the role of perilipin 3 in these disorders can lead to better therapeutic strategies targeting metabolic dysfunctions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Structural component of lipid droplets, which is required for the formation and maintenance of lipid storage droplets. Required for the transport of mannose 6-phosphate receptors (MPR) from endosomes to the trans-Golgi network.

See full target information Plin3

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 15:5489 PubMed38942786

2024

A fluorescent perilipin 2 knock-in mouse model reveals a high abundance of lipid droplets in the developing and adult brain.

Applications

Unspecified application

Species

Unspecified reactive species

Sofia Madsen,Ana C Delgado,Christelle Cadilhac,Vanille Maillard,Fabrice Battiston,Carla Marie Igelbüscher,Simon De Neck,Elia Magrinelli,Denis Jabaudon,Ludovic Telley,Fiona Doetsch,Marlen Knobloch

Journal of experimental zoology. Part A, Ecological and integrative physiology 337:1025-1038 PubMed35927786

2022

High-fat diet affects autophagy and mitochondrial compartment in rat Harderian gland.

Applications

Unspecified application

Species

Unspecified reactive species

Gabriella Chieffi Baccari,Sara Falvo,Maria M Di Fiore,Federica Cioffi,Antonia Giacco,Alessandra Santillo

Cell reports 24:2957-2971.e6 PubMed30208320

2018

GPS2 Deficiency Triggers Maladaptive White Adipose Tissue Expansion in Obesity via HIF1A Activation.

Applications

Unspecified application

Species

Unspecified reactive species

Karima Drareni,Raphaëlle Ballaire,Serena Barilla,Mano J Mathew,Amine Toubal,Rongrong Fan,Ning Liang,Catherine Chollet,Zhiqiang Huang,Maria Kondili,Fabienne Foufelle,Antoine Soprani,Ronan Roussel,Jean-François Gautier,Fawaz Alzaid,Eckardt Treuter,Nicolas Venteclef

The Journal of biological chemistry 290:25686-99 PubMed26304121

2015

Postnatal Deletion of Fat Storage-inducing Transmembrane Protein 2 (FIT2/FITM2) Causes Lethal Enteropathy.

Applications

Unspecified application

Species

Unspecified reactive species

Vera J Goh,Jolene S Y Tan,Bryan C Tan,Colin Seow,Wei-Yi Ong,Yen Ching Lim,Lei Sun,Sujoy Ghosh,David L Silver

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Perilipin 3/TIP47 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Perilipin 3/TIP47 antibody (AB118605)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Perilipin 3/TIP47 antibody (AB118605)

Western blot - Anti-Perilipin 3/TIP47 antibody (AB118605)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

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