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AB224344

Anti-Perilipin 3/TIP47 antibody

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(2 Publications)

Rabbit Polyclonal Perilipin 3/TIP47 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human PLIN3 aa 250-400.

View Alternative Names

M6PRBP1, TIP47, PLIN3, Perilipin-3, 47 kDa mannose 6-phosphate receptor-binding protein, Cargo selection protein TIP47, Mannose-6-phosphate receptor-binding protein 1, Placental protein 17, 47 kDa MPR-binding protein, PP17

4 Images
Immunocytochemistry/ Immunofluorescence - Anti-Perilipin 3/TIP47 antibody (AB224344)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Perilipin 3/TIP47 antibody (AB224344)

PFA-fixed, Triton X-100 permeabilized A431 (human epidermoid carcinoma cell line) cells stained for Perilipin 3/TIP47 (green) using ab224344 (4 μg/ml) in ICC/IF.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Perilipin 3/TIP47 antibody (AB224344)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Perilipin 3/TIP47 antibody (AB224344)

Paraffin-embedded human small intestine tissue stained for Perilipin 3/TIP47 with ab224344 (1/200) in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Perilipin 3/TIP47 antibody (AB224344)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Perilipin 3/TIP47 antibody (AB224344)

Paraffin-embedded human placenta tissue stained for Perilipin 3/TIP47 with ab224344 (1/200) in immunohistochemical analysis.

Western blot - Anti-Perilipin 3/TIP47 antibody (AB224344)
  • WB

Supplier Data

Western blot - Anti-Perilipin 3/TIP47 antibody (AB224344)

All lanes:

Western blot - Anti-Perilipin 3/TIP47 antibody (ab224344) at 1/100 dilution

All lanes:

U-87 MG (human glioblastoma-astrocytoma epithelial cell line) cell lysate

Predicted band size: 47 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human PLIN3 aa 250-400. The exact immunogen used to generate this antibody is proprietary information.

O60664

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Perilipin 3 also known as TIP47 is a lipid droplet-associated protein with a molecular weight of approximately 47 kDa. This protein expresses in a variety of tissues especially those involved in lipid metabolism like adipose tissue and liver. Perilipin 3 localizes on the surface of lipid droplets where it plays an important role in managing lipid storage and mobilization. This protein interacts directly with the lipid droplet surface facilitating stabilization and regulation of lipid content.
Biological function summary

Perilipin 3 acts as a mediator in lipid droplet dynamics and is part of the perilipin family of proteins. These proteins are key modulators of lipid storage and release ensuring balance within lipid metabolism. Perilipin 3 does not form a complex but has functional interactions with other perilipin proteins like perilipin 1 and perilipin 2 which synergize in the regulation of lipid droplets. This interaction ensures the efficiency of lipid metabolism processes and energy homeostasis in cells.

Pathways

Perilipin 3 participates in lipid metabolism and energy homeostasis pathways. It works alongside proteins such as adipose triglyceride lipase (ATGL) and hormone-sensitive lipase (HSL) to manage the lipolytic process. This involves the breakdown of stored triglycerides into free fatty acids and glycerol. The protein is integral in the regulation of lipid storage and catabolism pathways facilitating energy release during increased energy demand conditions.

Perilipin 3 has connections to metabolic diseases such as obesity and lipid storage disorders. Aberrant function or expression of perilipin 3 can lead to disrupted lipid metabolism and contribute to these conditions. Research shows that perilipin 3 works with proteins like ATGL in these contexts as alterations in their activities affect lipid handling. Understanding the role of perilipin 3 in these disorders can lead to better therapeutic strategies targeting metabolic dysfunctions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Structural component of lipid droplets, which is required for the formation and maintenance of lipid storage droplets (PubMed : 34077757). Required for the transport of mannose 6-phosphate receptors (MPR) from endosomes to the trans-Golgi network (PubMed : 9590177).
See full target information PLIN3
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cancer immunology, immunotherapy : CII 73:78 PubMed38554152

2024

Lipid droplets-related Perilipin-3: potential immune checkpoint and oncogene in oral squamous cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Yijia He,Lingyun Liu,Yuexin Dong,Xiaoxin Zhang,Yuxian Song,Yue Jing,Yanhong Ni,Yi Wang,Zhiyong Wang,Liang Ding

International journal of molecular sciences 21: PubMed32106593

2020

BDNF Expression in Cortical GABAergic Interneurons.

Applications

Unspecified application

Species

Unspecified reactive species

Federico José Barreda Tomás,Paul Turko,Heike Heilmann,Thorsten Trimbuch,Yuchio Yanagawa,Imre Vida,Agnieszka Münster-Wandowski
View all publications
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Product promise

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