AB47638

Anti-Perilipin 3/TIP47 antibody

Name: Anti-Perilipin 3/TIP47 antibody (ab47638) | Abcam
Brand: Abcam Limited
SKU: AB47638
Price: 525 USD
Availability: InStock
Rating: 4 (1 reviews)

(1 Review)

(17 Publications)

Rabbit Polyclonal Perilipin 3/TIP47 antibody. Suitable for WB, IHC-P and reacts with Rat, Human samples. Cited in 17 publications.

View Alternative Names

M6PRBP1, TIP47, PLIN3, Perilipin-3, 47 kDa mannose 6-phosphate receptor-binding protein, Cargo selection protein TIP47, Mannose-6-phosphate receptor-binding protein 1, Placental protein 17, 47 kDa MPR-binding protein, PP17

4 Images

Immunocytochemistry/ Immunofluorescence - Anti-Perilipin 3/TIP47 antibody (AB47638)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Perilipin 3/TIP47 antibody (AB47638)

Immunofluorescence of Perilipin 3/TIP47 in Rat Liver cells using ab47638 at 20 ug/ml.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Perilipin 3/TIP47 antibody (AB47638)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Perilipin 3/TIP47 antibody (AB47638)

ab47638 at 10 μg/ml staining Perilipin 3/TIP47 in rat liver tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded tissue sections).

Supplier Data

Western blot - Anti-Perilipin 3/TIP47 antibody (AB47638)

Diluting buffer and concentration : 5% NFDM/TBST.

Primary incubated for 1 hour at room temperature.

Lane 1:

Western blot - Anti-Perilipin 3/TIP47 antibody (ab47638) at 1 µg/mL

Lane 2:

Western blot - Anti-Perilipin 3/TIP47 antibody (ab47638) at 2 µg/mL

All lanes:

Daudi (human Burkitt's lymphoma cell line) cell lysate at 15 µg

Secondary

All lanes:

Goat Anti-rabbit IgG (HRP) at 1/10000 dilution

Predicted band size: 47 kDa

Observed band size: 47 kDa

false

Supplier Data

Western blot - Anti-Perilipin 3/TIP47 antibody (AB47638)

Diluting buffer and concentration : 5% NFDM/TBST.

Primary incubated for 1 hour at room temperature.

Lane 1:

Western blot - Anti-Perilipin 3/TIP47 antibody (ab47638) at 0.5 µg/mL

Lane 2:

Western blot - Anti-Perilipin 3/TIP47 antibody (ab47638) at 1 µg/mL

Lane 3:

Western blot - Anti-Perilipin 3/TIP47 antibody (ab47638) at 2 µg/mL

All lanes:

Rat Liver tissue lysate at 15 µg

Secondary

All lanes:

Goat Anti-rabbit IgG (HRP) at 1/10000 dilution

Predicted band size: 47 kDa

Observed band size: 47 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Rat, Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-4 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-4 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "10 µg/mL", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Perilipin 3 also known as TIP47 is a lipid droplet-associated protein with a molecular weight of approximately 47 kDa. This protein expresses in a variety of tissues especially those involved in lipid metabolism like adipose tissue and liver. Perilipin 3 localizes on the surface of lipid droplets where it plays an important role in managing lipid storage and mobilization. This protein interacts directly with the lipid droplet surface facilitating stabilization and regulation of lipid content.

Biological function summary

Perilipin 3 acts as a mediator in lipid droplet dynamics and is part of the perilipin family of proteins. These proteins are key modulators of lipid storage and release ensuring balance within lipid metabolism. Perilipin 3 does not form a complex but has functional interactions with other perilipin proteins like perilipin 1 and perilipin 2 which synergize in the regulation of lipid droplets. This interaction ensures the efficiency of lipid metabolism processes and energy homeostasis in cells.

Pathways

Perilipin 3 participates in lipid metabolism and energy homeostasis pathways. It works alongside proteins such as adipose triglyceride lipase (ATGL) and hormone-sensitive lipase (HSL) to manage the lipolytic process. This involves the breakdown of stored triglycerides into free fatty acids and glycerol. The protein is integral in the regulation of lipid storage and catabolism pathways facilitating energy release during increased energy demand conditions.

Perilipin 3 has connections to metabolic diseases such as obesity and lipid storage disorders. Aberrant function or expression of perilipin 3 can lead to disrupted lipid metabolism and contribute to these conditions. Research shows that perilipin 3 works with proteins like ATGL in these contexts as alterations in their activities affect lipid handling. Understanding the role of perilipin 3 in these disorders can lead to better therapeutic strategies targeting metabolic dysfunctions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Structural component of lipid droplets, which is required for the formation and maintenance of lipid storage droplets (PubMed : 34077757). Required for the transport of mannose 6-phosphate receptors (MPR) from endosomes to the trans-Golgi network (PubMed : 9590177).

See full target information PLIN3

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Publications (17)

Recent publications for all applications. Explore the full list and refine your search

Journal of neuroinflammation 22:117 PubMed40275347

2025

Astrocytic lipid droplets contain MHCII and may act as cogs in the antigen presentation machinery.

Applications

Unspecified application

Species

Unspecified reactive species

Chiara Beretta,Abdulkhalek Dakhel,Khalid Eltom,Fredrik Rosqvist,Simon Uzoni,Tobias Mothes,John S Fletcher,Ulf Risérus,Dag Sehlin,Jinar Rostami,Wojciech Piotr Michno,Anna Erlandsson

Investigative ophthalmology & visual science 65:12 PubMed39636725

2024

Atg5-Mediated Lipophagy Induces Ferroptosis in Corneal Epithelial Cells in Dry Eye Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Xin Zuo,Hao Zeng,Xue Yang,Dalian He,Bowen Wang,Jin Yuan

Journal of clinical pathology 77:330-337 PubMed36854623

2024

Lipid droplets' functional protein caveolin-2 is associated with lipid metabolism-related molecule FABP5 and EMT marker E-cadherin in oral epithelial dysplasia.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao-Jie Chen,Yu-Ting Bai,Ji-Rong Xie,Gang Zhou

Methods in molecular biology (Clifton, N.J.) 2879:151-171 PubMed38411886

2024

Visualizing Lipophagy as a New Mechanism of the Synthesis of Sex Steroids in Human Ovary and Testis Using Immunofluorescence Staining Method.

Applications

Unspecified application

Species

Unspecified reactive species

Yashar Esmaeilian,Sevgi Yusufoglu,Ece Iltumur,Gamze Bildik,Ozgur Oktem

Nature communications 15:79 PubMed38167301

2024

Cell cycle arrest induces lipid droplet formation and confers ferroptosis resistance.

Applications

Unspecified application

Species

Unspecified reactive species

Hyemin Lee,Amber Horbath,Lavanya Kondiparthi,Jitendra Kumar Meena,Guang Lei,Shayani Dasgupta,Xiaoguang Liu,Li Zhuang,Pranavi Koppula,Mi Li,Iqbal Mahmud,Bo Wei,Philip L Lorenzi,Khandan Keyomarsi,Masha V Poyurovsky,Kellen Olszewski,Boyi Gan

BMC cancer 23:677 PubMed37464334

2023

Transcriptional analysis of the expression and prognostic value of lipid droplet-localized proteins in hepatocellular carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Yize Zhang,Xue Liang,Qinghai Lian,Liwen Liu,Baoyu Zhang,Zihui Dong,Kunpeng Liu

International journal of molecular sciences 24: PubMed37372955

2023

Porcine Follicular Fluid-Derived Exosome: The Pivotal Material for Porcine Oocyte Maturation in Lipid Antioxidant Activity.

Applications

Unspecified application

Species

Unspecified reactive species

Euihyun Kim,Kihae Ra,Myung-Shin Lee,Geon A Kim

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 9:e2104291 PubMed36031387

2022

Trip13 Depletion in Liver Cancer Induces a Lipogenic Response Contributing to Plin2-Dependent Mitotic Cell Death.

Applications

Unspecified application

Species

Unspecified reactive species

Marcos Rios Garcia,Bettina Meissburger,Jessica Chan,Roldan M de Guia,Frits Mattijssen,Stephanie Roessler,Andreas L Birkenfeld,Nathanael Raschzok,Fabien Riols,Janina Tokarz,Maude Giroud,Manuel Gil Lozano,Goetz Hartleben,Peter Nawroth,Mark Haid,Miguel López,Stephan Herzig,Mauricio Berriel Diaz

BioMed research international 2022:4581405 PubMed35845956

2022

Perilipin 2 Protects against Lipotoxicity-Induced Islet Fibrosis by Inducing Islet Stellate Cell Activation Phenotype Changes.

Applications

Unspecified application

Species

Unspecified reactive species

Yunting Zhou,Yuming Wang,Chengming Ni,Huiying Wang,Junming Zhou,Bingying Wan,Huiqin Li,Fengfei Li,Rong Huang,Wei Xu,Ting Shan,Tingting Cai,Xiaoceng Kong,Bingli Liu,Xiaomei Liu,Zilin Sun,Jianhua Ma

iScience 25:104625 PubMed35800763

2022

Calpain-mediated cleavage generates a ZBTB18 N-terminal product that regulates HIF1A signaling and glioblastoma metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Anie P Masilamani,Rana Schulzki,Shuai Yuan,Ira V Haase,Eva Kling,Franziska Dewes,Geoffroy Andrieux,Melanie Börries,Oliver Schnell,Dieter H Heiland,Oliver Schilling,Roberto Ferrarese,Maria S Carro

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Perilipin 3/TIP47 antibody

Immunocytochemistry/ Immunofluorescence - Anti-Perilipin 3/TIP47 antibody (AB47638)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Perilipin 3/TIP47 antibody (AB47638)

Western blot - Anti-Perilipin 3/TIP47 antibody (AB47638)

Western blot - Anti-Perilipin 3/TIP47 antibody (AB47638)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

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Primary Antibodies

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Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (17)

Product promise