Rabbit Polyclonal Perilipin 3/TIP47 antibody. Suitable for WB, IHC-P and reacts with Rat, Human samples. Cited in 8 publications.
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- Journal of clinical pathology 77:330-3372024Lipid droplets' functional protein caveolin-2 is associated with lipid metabolism-related molecule FABP5 and EMT marker E-cadherin in oral epithelial dysplasia.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiao-Jie Chen,Yu-Ting Bai,Ji-Rong Xie,Gang ZhouPubMed 36854623
- BMC cancer 23:6772023Transcriptional analysis of the expression and prognostic value of lipid droplet-localized proteins in hepatocellular carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYize Zhang,Xue Liang,Qinghai Lian,Liwen Liu,Baoyu Zhang,Zihui Dong,Kunpeng LiuPubMed 37464334
- International journal of molecular sciences 24:2023Porcine Follicular Fluid-Derived Exosome: The Pivotal Material for Porcine Oocyte Maturation in Lipid Antioxidant Activity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEuihyun Kim,Kihae Ra,Myung-Shin Lee,Geon A KimPubMed 37372955
- Medical oncology (Northwood, London, England) 38:1162021Suppressed PLIN3 frequently occurs in prostate cancer, promoting docetaxel resistance via intensified autophagy, an event reversed by chloroquine.Applications:Unspecified applicationReactive species:Unspecified reactive speciesIoannis Lamprou,Avgi Tsolou,Christos Kakouratos,Achilleas G Mitrakas,Erasmia T Xanthopoulou,Katerina Kassela,Ioannis Karakasiliotis,Christos E Zois,Alexandra Giatromanolaki,Michael I KoukourakisPubMed 34410522
- Cell metabolism 32:229-242.e82020Targeting DGAT1 Ameliorates Glioblastoma by Increasing Fat Catabolism and Oxidative Stress.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiang Cheng et. al.PubMed 32559414
- Cell 178:275-289.e162019Viral Generated Inter-Organelle Contacts Redirect Lipid Flux for Genome Replication.Applications:Unspecified applicationReactive species:Unspecified reactive speciesOrly Laufman et. al.PubMed 31204099
- Journal of cellular biochemistry 120:343-3562018PNPLA3 variant M148 causes resistance to starvation-mediated lipid droplet autophagy in human hepatocytes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFlorentina Negoita,Julia Blomdahl,Sebastian Wasserstrom,Martin E Winberg,Peter Osmark,Sara Larsson,Karin G Stenkula,Mattias Ekstedt,Stergios Kechagias,Cecilia Holm,Helena A JonesPubMed 30171718
- Clinical cancer research : an official journal of 22:5337-53482016Inhibition of SOAT1 Suppresses Glioblastoma Growth via Blocking SREBP-1-Mediated Lipogenesis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFeng Geng et. al.PubMed 27281560
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Tested | Expected |
| Rat | Tested | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 0.50000-4.00000 µg/mL | Notes - |
Species Human | Dilution info 0.50000-4.00000 µg/mL | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 10 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
3 products for Alternative Product
Target data
Function
Structural component of lipid droplets, which is required for the formation and maintenance of lipid storage droplets (PubMed:34077757). Required for the transport of mannose 6-phosphate receptors (MPR) from endosomes to the trans-Golgi network (PubMed:9590177).
Alternative names
M6PRBP1, TIP47, PLIN3, Perilipin-3, 47 kDa mannose 6-phosphate receptor-binding protein, Cargo selection protein TIP47, Mannose-6-phosphate receptor-binding protein 1, Placental protein 17, 47 kDa MPR-binding protein, PP17
Recommended products
Rabbit Polyclonal Perilipin 3/TIP47 antibody. Suitable for WB, IHC-P and reacts with Rat, Human samples. Cited in 8 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Perilipin 3 also known as TIP47 is a lipid droplet-associated protein with a molecular weight of approximately 47 kDa. This protein expresses in a variety of tissues especially those involved in lipid metabolism like adipose tissue and liver. Perilipin 3 localizes on the surface of lipid droplets where it plays an important role in managing lipid storage and mobilization. This protein interacts directly with the lipid droplet surface facilitating stabilization and regulation of lipid content.
- Biological function summary
Perilipin 3 acts as a mediator in lipid droplet dynamics and is part of the perilipin family of proteins. These proteins are key modulators of lipid storage and release ensuring balance within lipid metabolism. Perilipin 3 does not form a complex but has functional interactions with other perilipin proteins like perilipin 1 and perilipin 2 which synergize in the regulation of lipid droplets. This interaction ensures the efficiency of lipid metabolism processes and energy homeostasis in cells.
- Pathways
Perilipin 3 participates in lipid metabolism and energy homeostasis pathways. It works alongside proteins such as adipose triglyceride lipase (ATGL) and hormone-sensitive lipase (HSL) to manage the lipolytic process. This involves the breakdown of stored triglycerides into free fatty acids and glycerol. The protein is integral in the regulation of lipid storage and catabolism pathways facilitating energy release during increased energy demand conditions.
- Associated diseases and disorders
Perilipin 3 has connections to metabolic diseases such as obesity and lipid storage disorders. Aberrant function or expression of perilipin 3 can lead to disrupted lipid metabolism and contribute to these conditions. Research shows that perilipin 3 works with proteins like ATGL in these contexts as alterations in their activities affect lipid handling. Understanding the role of perilipin 3 in these disorders can lead to better therapeutic strategies targeting metabolic dysfunctions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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3 product images
Western blot - Anti-Perilipin 3/TIP47 antibody (ab47638)
Diluting buffer and concentration: 5% NFDM/TBST.
Primary incubated for 1 hour at room temperature.
Lane 1: Western blot - Anti-Perilipin 3/TIP47 antibody (ab47638) at 1 µg/mL
Lane 2: Western blot - Anti-Perilipin 3/TIP47 antibody (ab47638) at 2 µg/mL
All lanes: Daudi (human Burkitt's lymphoma cell line) cell lysate at 15 µg
SecondaryAll lanes: Goat Anti-rabbit IgG (HRP) at 1/10000 dilution
Predicted band size: 47 kDa
Observed band size: 47 kDa
Western blot - Anti-Perilipin 3/TIP47 antibody (ab47638)
Diluting buffer and concentration: 5% NFDM/TBST.
Primary incubated for 1 hour at room temperature.
Lane 1: Western blot - Anti-Perilipin 3/TIP47 antibody (ab47638) at 0.5 µg/mL
Lane 2: Western blot - Anti-Perilipin 3/TIP47 antibody (ab47638) at 1 µg/mL
Lane 3: Western blot - Anti-Perilipin 3/TIP47 antibody (ab47638) at 2 µg/mL
All lanes: Rat Liver tissue lysate at 15 µg
SecondaryAll lanes: Goat Anti-rabbit IgG (HRP) at 1/10000 dilution
Predicted band size: 47 kDa
Observed band size: 47 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Perilipin 3/TIP47 antibody (ab47638)
ab47638 at 10 μg/ml staining Perilipin 3/TIP47 in rat liver tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded tissue sections).
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