Anti-Perilipin 3/TIP47 antibody

• WB

Supplier Data

Western blot - Anti-Perilipin 3/TIP47 antibody (AB47639)

Diluting buffer and concentration : 5% NFDM/TBST. Primary incubated for 1 hour at room temperature.

ab47639 can detect human isoforms 1, 3 and 4 which may account for the additional band.

All lanes:

Western blot - Anti-Perilipin 3/TIP47 antibody (ab47639) at 1 µg/mL

Lane 1:

CaCo-2 (human colorectal adenocarcinoma cell line) cell lysate at 15 µg

Lane 2:

Daudi (human Burkitt's lymphoma cell line) cell lysate at 15 µg

Lane 3:

HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate at 15 µg

Lane 4:

HepG2 (human liver hepatocellular carcinoma cell line) cell lysate at 15 µg

Lane 5:

K562 (human chronic myelogenous leukemia cell line from bone marrow ) cell lysate at 15 µg

Lane 6:

Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate at 15 µg

Lane 7:

SK-N-SH (human neuroblastoma cell line) cell lysate at 15 µg

Lane 8:

THP-1 (human monocytic leukemia cell line) cell lysate at 15 µg

Secondary

All lanes:

Goat Anti-rabbit IgG (HRP) at 1/10000 dilution

Predicted band size: 47 kDa

Observed band size: 47 kDa

false

• WB

Supplier Data

Western blot - Anti-Perilipin 3/TIP47 antibody (AB47639)

Diluting buffer and concentration : 5% NFDM/TBST. Primary incubated for 1 hour at room temperature.

All lanes:

Western blot - Anti-Perilipin 3/TIP47 antibody (ab47639) at 1 µg/mL

Lane 1:

NIH/3T3 (mouse embryo fibroblast cell line) cell lysate at 15 µg

Lane 2:

L1210 (mouse lymphoblastic leukemia cell line) cell lysate at 15 µg

Secondary

All lanes:

Goat Anti-rabbit IgG (HRP)

Predicted band size: 47 kDa

Observed band size: 47 kDa

false

• WB

Supplier Data

Western blot - Anti-Perilipin 3/TIP47 antibody (AB47639)

Diluting buffer and concentration : 5% NFDM/TBST. Primary incubated for 1 hour at room temperature.

Lane 1:

Western blot - Anti-Perilipin 3/TIP47 antibody (ab47639) at 1 µg/mL

Lane 2:

Western blot - Anti-Perilipin 3/TIP47 antibody (ab47639) at 2 µg/mL

All lanes:

EL4 (mouse T cell lymphoblast cell line) cell lysate at 15 µg

Predicted band size: 47 kDa

Observed band size: 47 kDa

false

• WB

CiteAb

Western blot - Anti-Perilipin 3/TIP47 antibody (AB47639)

Perilipin 3/TIP47 western blot using anti-Perilipin 3/TIP47 antibody ab47639. Publication image and figure legend from Vogt, D. A., Camus, G., et al., 2013, PLoS Pathog, PubMed 23593007.

ab47639 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab47639 please see the product overview.

TIP47 is required for HCV RNA replication.A) Schematic representation of HCV Replicon (Con1, genotype 1b), a subgenomic bicistronic HCV replicon with neomycin as a reporter gene, and Luc-JFH1 (genotype 2a), a full-length bicistronic HCV infectious clone with firefly luciferase as a reporter gene. B) Crystal violet-stained Huh7.5 cells transfected with control shRNA or shRNA against TIP47 and HCV replicon RNA (A). G418 was added to the medium (1 mg/ml). Cells were kept under selective pressure for 4 weeks. Only cells actively replicating HCV RNA, and thus expressing the neomycin phosphotransferase survive. C) Quantification of colony numbers/plate in (B) (mean ± standard deviation; n = 3, *p<0.05). D) Western blot analysis of TIP47 expression in shRNA-expressing Huh7.5 cells at the time of transfection in (B). E) Transfection of shRNA-expressing Huh7.5 cells with Luc-JFH1 RNA (A) and Myc-TIP47 expression plasmid (where indicated). HCV replication was measured by determining the fold increase of luciferase (RLU) per well over a 4 h time point (mean ± standard deviation; n = 3; *p<0.05, **p<0.01). F) Western blot analysis of TIP47 expression in shRNA-expressing Huh7.5 cells at time of transfection in (E).

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