AB219056

Anti-Periostin antibody [EPR19934]

RabMAb
Recombinant
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(4 Publications)

Rabbit Recombinant Monoclonal Periostin antibody. Suitable for IP, WB, IHC-P and reacts with Human samples. Cited in 4 publications.

View Alternative Names

OSF2, POSTN, Periostin, PN, Osteoblast-specific factor 2, OSF-2

6 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Periostin antibody [EPR19934] (AB219056)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Periostin antibody [EPR19934] (AB219056)

Immunohistochemical analysis of paraffin-embedded human colon tissue labeling Periostin with ab219056 at 1/2000 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Extracellular matrix staining on human colon (PMID : 26023718).

Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Periostin antibody [EPR19934] (AB219056)

Immunohistochemical analysis of paraffin-embedded human lung cancer tissue labeling Periostin with ab219056 at 1/2000 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Extracellular matrix staining on human lung cancer (PMID : 24273600).

Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Supplier Data

Immunoprecipitation - Anti-Periostin antibody [EPR19934] (AB219056)

Periostin was immunoprecipitated from 0.35 mg of Human breast cancer lysate with ab219056 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab219056 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

Lane 1 : Human breast cancer lysate 10 μg (Input).

Lane 2 : ab219056 IP in Human breast cancer lysate.

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab219056 in Human breast cancer lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 30 seconds.

All lanes:

Immunoprecipitation - Anti-Periostin antibody [EPR19934] (ab219056)

Predicted band size: 93 kDa

false

Supplier Data

Western blot - Anti-Periostin antibody [EPR19934] (AB219056)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Periostin antibody [EPR19934] (ab219056) at 1/1000 dilution

All lanes:

Human breast cancer lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Predicted band size: 93 kDa

Observed band size: 93 kDa

false

Exposure time: 3min

Supplier Data

Western blot - Anti-Periostin antibody [EPR19934] (AB219056)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression tissues : liver, pancreas (PMID : 15514205, PMID : 29163770).

This antibody is not suitable for cell line testing.

All lanes:

Western blot - Anti-Periostin antibody [EPR19934] (ab219056) at 1/1000 dilution

Lane 1:

Human colon tissue lysate at 20 µg

Lane 2:

Human liver tissue lysate at 20 µg

Lane 3:

Human pancreas tissue lysate at 20 µg

Lane 4:

A549 (human lung carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 5:

MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 6:

MDA-MB-468 (human breast adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 7:

MG-63 (human osteosarcoma fibroblast) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 93 kDa

Observed band size: 93 kDa

false

Exposure time: 180s

Supplier Data

Western blot - Anti-Periostin antibody [EPR19934] (AB219056)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Periostin antibody [EPR19934] (ab219056) at 1/1000 dilution

Lane 1:

Human fetal heart tissue lysate at 10 µg

Lane 2:

Human fetal kidney tissue lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

Predicted band size: 93 kDa

Observed band size: 93 kDa

false

Exposure time: 3min

660 APC

APC Anti-Periostin antibody [EPR19934]
578 PE

PE Anti-Periostin antibody [EPR19934]
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-Periostin antibody [EPR19934]
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-Periostin antibody [EPR19934]
617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-Periostin antibody [EPR19934]
565 Alexa Fluor® 555

Alexa Fluor® 555 Anti-Periostin antibody [EPR19934]
775 Alexa Fluor® 750

Alexa Fluor® 750 Anti-Periostin antibody [EPR19934]
Carrier free

Anti-Periostin antibody [EPR19934] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR19934

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

This antibody recognizes a different epitope of Periostin compared to ab219057.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "This antibody is not suitable for cell line testing in WB", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/2000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Periostin also known as POSTN or OSF-2 is an extracellular matrix protein with a molecular weight of approximately 90 kDa. It plays a mechanical role in tissue remodeling by binding to integrins which supports tissue repair and regeneration. Periostin is mainly expressed in collagen-rich tissues such as bone periodontal ligament and cardiac tissues. It is synthesized by osteoblasts fibroblasts and other stromal cells functioning as a structural protein that supports cell adhesion and migration.

Biological function summary

The periostin protein contributes to the maintenance of tissue integrity and mechanical properties. It does not solely act; it forms part of a multiprotein complex with collagen and other extracellular matrix components. This involvement is necessary for maintaining cellular interactions and promoting tissue healing processes. Additionally periostin influences cell proliferation and migration impacting developmental and wound healing biology.

Pathways

Periostin is involved in the integrin signaling pathway and the Wnt/β-catenin pathway both important for cell communication and movement. Through these pathways periostin interacts with integrins αvβ3 and αvβ5 facilitating cell adhesion and migration. The Wnt/β-catenin pathway interaction involves several proteins including Frizzled receptors ensuring regulation of cellular proliferation and differentiation. These pathways make periostin a coordinator of complex cellular processes.

The periostin protein is associated with conditions such as fibrotic diseases and cardiovascular disorders. In fibrosis periostin promotes collagen cross-linking exacerbating tissue stiffness. This involves increased interaction with proteins like collagen types I and V contributing to pathological fibrotic progression. In cardiovascular disorders elevated periostin levels after myocardial injury are linked with fibrotic scarring where it collaborates with matrix metalloproteinases to remodel the cardiac tissue architecture. Understanding these connections can lead to novel therapeutic strategies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Induces cell attachment and spreading and plays a role in cell adhesion (PubMed : 12235007). Enhances incorporation of BMP1 in the fibronectin matrix of connective tissues, and subsequent proteolytic activation of lysyl oxidase LOX (By similarity).

See full target information POSTN

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

International journal of biological sciences 21:3573-3596 PubMed40520021

2025

Integrative Single-Cell and Spatial Transcriptomics Analysis Reveals ECM-remodeling Cancer-associated Fibroblast-Derived POSTN as a Key Mediator in Pancreatic Ductal Adenocarcinoma Progression.

Applications

Unspecified application

Species

Unspecified reactive species

Yifan Wu,Shuquan Li,Hao Yu,Sha Zhang,Liang Yan,Xiaoya Guan,Wei Xu,Zhen Wang,Ang Lv,Xiuyun Tian,Chunyi Hao,Jianhui Wu

Translational oncology 52:102282 PubMed39808844

2025

A practical distribution pattern of α-SMA-positive carcinoma associated fibroblasts indicates poor prognosis of patients with pancreatic ductal adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Bo Li,Meilong Shi,Yang Wang,Penghao Li,Xiaoyi Yin,Guoxiao Zhang,Xiaochao Kang,Huan Wang,Suizhi Gao,Kailian Zheng,Xiaohan Shi,Xiongfei Xu,Yukun Zhou,Hui Jiang,Wei Jing,Shiwei Guo,Gang Jin

Experimental and therapeutic medicine 22:1023 PubMed34373709

2021

mRNA expression level of , , , and modulates 5-fluorouracil resistance in colon cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Tao Liu,Rongmu Xia,Chenmeng Li,Xiaocong Chen,Xuemin Cai,Wengang Li

Cell death & disease 9:1082 PubMed30348980

2018

Periostin secreted by cancer-associated fibroblasts promotes cancer stemness in head and neck cancer by activating protein tyrosine kinase 7.

Applications

Unspecified application

Species

Unspecified reactive species

Binbin Yu,Kailiu Wu,Xu Wang,Jianjun Zhang,Lizhen Wang,Yingying Jiang,Xueqin Zhu,Wantao Chen,Ming Yan

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Periostin antibody [EPR19934]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Periostin antibody [EPR19934] (AB219056)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Periostin antibody [EPR19934] (AB219056)

Immunoprecipitation - Anti-Periostin antibody [EPR19934] (AB219056)

Western blot - Anti-Periostin antibody [EPR19934] (AB219056)

Western blot - Anti-Periostin antibody [EPR19934] (AB219056)

Western blot - Anti-Periostin antibody [EPR19934] (AB219056)

Related conjugates and formulations (8)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

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