Anti-Periostin antibody [EPR19936]

• IP

Supplier Data

Immunoprecipitation - Anti-Periostin antibody [EPR19936] (AB219057)

Periostin was immunoprecipitated from 0.35 mg of Human fetal heart lysate with ab219057 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab219057 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

Lane 1 : Human fetal heart lysate 10 μg (Input).

Lane 2 : ab219057 IP in Human fetal heart lysate.

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab219057 in Human fetal heart lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 3 minutes.

All lanes:

Immunoprecipitation - Anti-Periostin antibody [EPR19936] (ab219057)

Predicted band size: 93 kDa

false

• WB

Supplier Data

Western blot - Anti-Periostin antibody [EPR19936] (AB219057)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Periostin antibody [EPR19936] (ab219057) at 1/1000 dilution

Lane 1:

Human fetal heart lysate at 10 µg

Lane 2:

Human fetal kidney lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

Predicted band size: 93 kDa

Observed band size: 93 kDa

false

Exposure time: 30s

• WB

Supplier Data

Western blot - Anti-Periostin antibody [EPR19936] (AB219057)

Blocking/Dilution buffer : 5% NFDM/TBST.

Exposure time : Lane 1-3 : 30 seconds; Lane 4 : 5 seconds.

All lanes:

Western blot - Anti-Periostin antibody [EPR19936] (ab219057) at 1/1000 dilution

Lane 1:

Human colon cancer lysate at 20 µg

Lane 2:

Human stomach lysate at 20 µg

Lane 3:

HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg

Lane 4:

Human breast cancer lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 93 kDa

Observed band size: 93 kDa

false

• WB

Supplier Data

Western blot - Anti-Periostin antibody [EPR19936] (AB219057)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression tissues : liver, pancreas (PMID : 15514205, PMID : 29163770).

This antibody is not suitable for cell line testing.

All lanes:

Western blot - Anti-Periostin antibody [EPR19936] (ab219057) at 1/1000 dilution

Lane 1:

Human colon tissue lysate at 20 µg

Lane 2:

Human liver tissue lysate at 20 µg

Lane 3:

Human pancreas tissue lysate at 20 µg

Lane 4:

A549 (human lung carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 5:

MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 6:

MDA-MB-468 (human breast adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 7:

MG-63 (human osteosarcoma fibroblast) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 93 kDa

Observed band size: 93 kDa

false

Exposure time: 180s