Rabbit Recombinant Monoclonal Periostin antibody. Carrier free. Suitable for IHC-Fr, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 4 publications.
Images
![Immunohistochemistry (Frozen sections) - Anti-Periostin antibody [EPR20806] - BSA and Azide free (AB227049), expandable thumbnail](https://content.abcam.com/products/images/periostin-antibody-epr20806-bsa-and-azide-free-ab227049--immunohistochemistry-frozen-sections-img117077.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Frozen sections) - Anti-Periostin antibody [EPR20806] - BSA and Azide free (AB227049)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Periostin antibody [EPR20806] - BSA and Azide free (AB227049), expandable thumbnail](https://content.abcam.com/products/images/periostin-antibody-epr20806-bsa-and-azide-free-ab227049--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img7773.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Periostin antibody [EPR20806] - BSA and Azide free (AB227049)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Periostin antibody [EPR20806] - BSA and Azide free (AB227049), expandable thumbnail](https://content.abcam.com/products/images/periostin-antibody-epr20806-bsa-and-azide-free-ab227049--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img71095.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Periostin antibody [EPR20806] - BSA and Azide free (AB227049)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Periostin antibody [EPR20806] - BSA and Azide free (AB227049), expandable thumbnail](https://content.abcam.com/products/images/periostin-antibody-epr20806-bsa-and-azide-free-ab227049--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img49587.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Periostin antibody [EPR20806] - BSA and Azide free (AB227049)")
![Immunohistochemistry (Frozen sections) - Anti-Periostin antibody [EPR20806] - BSA and Azide free (AB227049), expandable thumbnail](https://content.abcam.com/products/images/periostin-antibody-epr20806-bsa-and-azide-free-ab227049--immunohistochemistry-frozen-sections-img115527.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Frozen sections) - Anti-Periostin antibody [EPR20806] - BSA and Azide free (AB227049)")
Publications
Filter by
- The Journal of clinical investigation 133:2023Improving radiotherapy in immunosuppressive microenvironments by targeting complement receptor C5aR1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCallum Beach,David MacLean,Dominika Majorova,Stavros Melemenidis,Dhanya K Nambiar,Ryan K Kim,Gabriel N Valbuena,Silvia Guglietta,Carsten Krieg,Mahnaz Darvish-Damavandi,Tatsuya Suwa,Alistair Easton,Lily Vs Hillson,Ashley K McCulloch,Ross K McMahon,Kathryn Pennel,Joanne Edwards,Sean M O'Cathail,Campbell S Roxburgh,Enric Domingo,Eui Jung Moon,Dadi Jiang,Yanyan Jiang,Qingyang Zhang,Albert C Koong,Trent M Woodruff,Edward E Graves,Tim Maughan,Simon Ja Buczacki,Manuel Stucki,Quynh-Thu Le,Simon J Leedham,Amato J Giaccia,Monica M OlcinaPubMed 37824211
- Nature 611:603-6132022Metastatic recurrence in colorectal cancer arises from residual EMP1 cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAdrià Cañellas-Socias,Carme Cortina,Xavier Hernando-Momblona,Sergio Palomo-Ponce,Eoghan J Mulholland,Gemma Turon,Lidia Mateo,Sefora Conti,Olga Roman,Marta Sevillano,Felipe Slebe,Diana Stork,Adrià Caballé-Mestres,Antonio Berenguer-Llergo,Adrián Álvarez-Varela,Nicola Fenderico,Laura Novellasdemunt,Laura Jiménez-Gracia,Tamara Sipka,Lidia Bardia,Patricia Lorden,Julien Colombelli,Holger Heyn,Xavier Trepat,Sabine Tejpar,Elena Sancho,Daniele V F Tauriello,Simon Leedham,Camille Stephan-Otto Attolini,Eduard BatllePubMed 36352230
- Cell stem cell 29:1213-1228.e82022Dynamic and adaptive cancer stem cell population admixture in colorectal neoplasia.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEster Gil Vasquez,Nadia Nasreddin,Gabriel N Valbuena,Eoghan J Mulholland,Hayley L Belnoue-Davis,Holly R Eggington,Ryan O Schenck,Valérie M Wouters,Pratyaksha Wirapati,Kathryn Gilroy,Tamsin R M Lannagan,Dustin J Flanagan,Arafath K Najumudeen,Sulochana Omwenga,Amy M B McCorry,Alistair Easton,Viktor H Koelzer,James E East,Dion Morton,Livio Trusolino,Timothy Maughan,Andrew D Campbell,Maurice B Loughrey,Philip D Dunne,Petros Tsantoulis,David J Huels,Sabine Tejpar,Owen J Sansom,Simon J LeedhamPubMed 35931031
- Scientific reports 10:139392020Multi-omic single cell analysis resolves novel stromal cell populations in healthy and diseased human tendon.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAdrian R Kendal et. al.PubMed 32883960
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-Fr | IHC-P | |
|---|---|---|
| Human | Expected | Tested |
| Mouse | Tested | Tested |
| Rat | Tested | Tested |
IHC-Fr
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Associated Products
Select an associated product type
6 products for Alternative Product
2 products for Alternative Version
Target data
Function
Induces cell attachment and spreading and plays a role in cell adhesion (PubMed:12235007). Enhances incorporation of BMP1 in the fibronectin matrix of connective tissues, and subsequent proteolytic activation of lysyl oxidase LOX (By similarity).
Alternative names
OSF2, POSTN, Periostin, PN, Osteoblast-specific factor 2, OSF-2
Recommended products
Rabbit Recombinant Monoclonal Periostin antibody. Carrier free. Suitable for IHC-Fr, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 4 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR20806
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab227049 is the carrier-free version of Anti-Periostin antibody [EPR20806] ab215199.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Periostin also known as POSTN or OSF-2 is an extracellular matrix protein with a molecular weight of approximately 90 kDa. It plays a mechanical role in tissue remodeling by binding to integrins which supports tissue repair and regeneration. Periostin is mainly expressed in collagen-rich tissues such as bone periodontal ligament and cardiac tissues. It is synthesized by osteoblasts fibroblasts and other stromal cells functioning as a structural protein that supports cell adhesion and migration.
- Biological function summary
The periostin protein contributes to the maintenance of tissue integrity and mechanical properties. It does not solely act; it forms part of a multiprotein complex with collagen and other extracellular matrix components. This involvement is necessary for maintaining cellular interactions and promoting tissue healing processes. Additionally periostin influences cell proliferation and migration impacting developmental and wound healing biology.
- Pathways
Periostin is involved in the integrin signaling pathway and the Wnt/β-catenin pathway both important for cell communication and movement. Through these pathways periostin interacts with integrins αvβ3 and αvβ5 facilitating cell adhesion and migration. The Wnt/β-catenin pathway interaction involves several proteins including Frizzled receptors ensuring regulation of cellular proliferation and differentiation. These pathways make periostin a coordinator of complex cellular processes.
- Associated diseases and disorders
The periostin protein is associated with conditions such as fibrotic diseases and cardiovascular disorders. In fibrosis periostin promotes collagen cross-linking exacerbating tissue stiffness. This involves increased interaction with proteins like collagen types I and V contributing to pathological fibrotic progression. In cardiovascular disorders elevated periostin levels after myocardial injury are linked with fibrotic scarring where it collaborates with matrix metalloproteinases to remodel the cardiac tissue architecture. Understanding these connections can lead to novel therapeutic strategies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
6 product images
![Immunohistochemistry (Frozen sections) - Anti-Periostin antibody [EPR20806] - BSA and Azide free (ab227049), expandable thumbnail](https://content.abcam.com/products/images/periostin-antibody-epr20806-bsa-and-azide-free-ab227049--immunohistochemistry-frozen-sections-img117077.jpg "Immunohistochemistry (Frozen sections) - Anti-Periostin antibody [EPR20806] - BSA and Azide free (ab227049)")
Immunohistochemistry (Frozen sections) - Anti-Periostin antibody [EPR20806] - BSA and Azide free (ab227049)
Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized mouse stomach tissue labeling Periostin with Anti-Periostin antibody [EPR20806] ab215199 at 1/500 dilution ,followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green). Positive cytoplasmic staining in the gastric lamina propia mucosae, negative staining in the epithelial cells (PMID: 24418260).
The nuclear counter stain is DAPI (blue).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Periostin antibody [EPR20806] ab215199).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Periostin antibody [EPR20806] - BSA and Azide free (ab227049), expandable thumbnail](https://content.abcam.com/products/images/periostin-antibody-epr20806-bsa-and-azide-free-ab227049--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img7773.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Periostin antibody [EPR20806] - BSA and Azide free (ab227049)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Periostin antibody [EPR20806] - BSA and Azide free (ab227049)
Immunohistochemical analysis of paraffin-embedded human stomach tissue labeling Periostin with Anti-Periostin antibody [EPR20806] ab215199 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive extracellular matrix staining on the stroma of human stomach (PMID: 15731169). Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Periostin antibody [EPR20806] ab215199).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Periostin antibody [EPR20806] - BSA and Azide free (ab227049), expandable thumbnail](https://content.abcam.com/products/images/periostin-antibody-epr20806-bsa-and-azide-free-ab227049--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img71095.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Periostin antibody [EPR20806] - BSA and Azide free (ab227049)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Periostin antibody [EPR20806] - BSA and Azide free (ab227049)
Immunohistochemical analysis of paraffin-embedded mouse colon tissue labeling Periostin with Anti-Periostin antibody [EPR20806] ab215199 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive extracellular matrix staining on the stroma of mouse colon (PMID: 15731169, PMID: 18443362). Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Periostin antibody [EPR20806] ab215199).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Periostin antibody [EPR20806] - BSA and Azide free (ab227049), expandable thumbnail](https://content.abcam.com/products/images/periostin-antibody-epr20806-bsa-and-azide-free-ab227049--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img49587.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Periostin antibody [EPR20806] - BSA and Azide free (ab227049)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Periostin antibody [EPR20806] - BSA and Azide free (ab227049)
Immunohistochemical analysis of paraffin-embedded rat stomach tissue labeling Periostin with Anti-Periostin antibody [EPR20806] ab215199 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive extracellular matrix staining on the stroma of rat stomach (PMID: 15731169). Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Periostin antibody [EPR20806] ab215199).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunohistochemistry (Frozen sections) - Anti-Periostin antibody [EPR20806] - BSA and Azide free (ab227049), expandable thumbnail](https://content.abcam.com/products/images/periostin-antibody-epr20806-bsa-and-azide-free-ab227049--immunohistochemistry-frozen-sections-img115527.jpg "Immunohistochemistry (Frozen sections) - Anti-Periostin antibody [EPR20806] - BSA and Azide free (ab227049)")
Immunohistochemistry (Frozen sections) - Anti-Periostin antibody [EPR20806] - BSA and Azide free (ab227049)
Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized rat stomach tissue labeling Periostin with Anti-Periostin antibody [EPR20806] ab215199 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green). Positive cytoplasmic staining in the gastric lamina propia mucosae, negative staining in the epithelial cells (PMID: 24418260).
The nuclear counter stain is DAPI (blue).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Periostin antibody [EPR20806] ab215199).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Periostin antibody [EPR20806] - BSA and Azide free (ab227049), expandable thumbnail](https://content.abcam.com/products/images/periostin-antibody-epr20806-bsa-and-azide-free-ab227049--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img418520.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Periostin antibody [EPR20806] - BSA and Azide free (ab227049)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Periostin antibody [EPR20806] - BSA and Azide free (ab227049)
Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labelling Periostin with Anti-Periostin antibody [EPR20806] ab215199 at 1/1000 dilution (0.516 μg/ml) followed by a Goat Anti-Rabbit IgG H&L (HRP polymer) (Goat Anti-Rabbit IgG H&L (HRP polymer) ab214880) at a Ready to use dilution. Positive staining on extracellular matrix of human breast carcinoma. The section was incubated with Anti-Periostin antibody [EPR20806] ab215199 at 4°C overnight. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (HRP polymer) (Goat Anti-Rabbit IgG H&L (HRP polymer) ab214880) at Ready to use dilution.
Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Periostin antibody [EPR20806] ab215199).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Periostin antibody [EPR20806] - BSA and Azide free (ab227049), expandable thumbnail](https://content.abcam.com/products/images/periostin-antibody-epr20806-bsa-and-azide-free-ab227049--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img418520.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Periostin antibody [EPR20806] - BSA and Azide free (ab227049)")
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com