AB254137

Anti-Peripherin antibody [7C5]

Name: Anti-Peripherin antibody [7C5] (ab254137) | Abcam
Brand: Abcam Limited
SKU: AB254137
Price: 525 USD
Availability: InStock

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(1 Publication)

Mouse Monoclonal Peripherin antibody. Suitable for WB, ICC/IF and reacts with Rat samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Rat Prph.

View Alternative Names

Prph1, Prph, Peripherin

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-Peripherin antibody [7C5] (AB254137)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Peripherin antibody [7C5] (AB254137)

Cultured newborn rat neurons and glia staining Peripherin using ab254137 (green) at a 1/200 dilution in ICC/IF. Neurofilament L in red.

Supplier Data

Western blot - Anti-Peripherin antibody [7C5] (AB254137)

All lanes:

Western blot - Anti-Peripherin antibody [7C5] (ab254137) at 1/1000 dilution

All lanes:

Rat cerebellar lysate

Predicted band size: 53 kDa

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Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

7C5

Isotype

IgG1

Carrier free

Reacts with

Rat

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Rat Prph. The exact immunogen used to generate this antibody is proprietary information.

P21807

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/200", "ICCIF-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Purification notes

The antibody is unpurified, concentrated culture supernatant.

Storage buffer

Preservative: 0.065% Sodium azide

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Peripherin also known as 8G2 or 7C5 is a protein expressed strongly in peripheral and certain central nervous system neurons. The mass of the peripherin protein is approximately 57 kDa. This intermediate filament protein structurally supports neurons aiding in their proper formation maintenance and regeneration. Peripherin localizes to the axons of mature neurons and serves important functions in the neuronal cytoskeleton and its stability.

Biological function summary

Peripherin impacts the nervous system's structure and dynamics. As part of the cytoskeleton it contributes to neurofilament networks which are essential for neuronal shape and mechanical integrity. Peripherin plays a role in neuronal differentiation and apoptosis. It operates independently but occasionally interacts with other cytoskeletal proteins like neurofilament light protein to perform its duties in neuronal cells.

Pathways

Peripherin is involved in processes associated with neuronal development and injury response. It features in neurogenesis pathways where it influences the growth and repair of axons. Peripherin interacts with proteins such as vimentin which helps manage cell dynamics and stress responses. The axonal transport pathway also utilizes peripherin to support axonal transport under stressful conditions ensuring cellular integrity and function.

Peripherin connects strongly to neurodegenerative diseases such as amyotrophic lateral sclerosis (ALS) and spinal muscular atrophy (SMA). Its overexpression or aggregation can create abnormal filamentous structures that contribute to motor neuron degeneration. In ALS peripherin may interact with TAR DNA-binding protein 43 (TDP-43) and superoxide dismutase 1 (SOD1) proteins known to be significant in the disease pathogenesis. Understanding peripherin’s role in these diseases aids in unraveling mechanisms underlying neuronal degeneration.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Class-III neuronal intermediate filament protein (PubMed : 2624740, PubMed : 3339087). My form an independent structural network without the involvement of other neurofilaments or may cooperate with the neuronal intermediate filament proteins NEFL, NEFH, NEFM and INA to form a filamentous network (By similarity). Assembly of the neuronal intermediate filaments may be regulated by RAB7A (By similarity). Plays a role in the development of unmyelinated sensory neurons (By similarity). May be involved in axon elongation and axon regeneration after injury (PubMed : 2585054, PubMed : 7530776). Inhibits neurite extension in type II spiral ganglion neurons in the cochlea (By similarity).

See full target information Prph

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

The Journal of physiology 601:1407-1424 PubMed36869605

2023

Antagonism of TRPV4 channels partially reduces mechanotransduction in rat skeletal muscle afferents.

Applications

Unspecified application

Species

Unspecified reactive species

Ayumi Fukazawa,Amane Hori,Norio Hotta,Kimiaki Katanosaka,Juan A Estrada,Rie Ishizawa,Han-Kyul Kim,Gary A Iwamoto,Scott A Smith,Wanpen Vongpatanasin,Masaki Mizuno

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Peripherin antibody [7C5]

Immunocytochemistry/ Immunofluorescence - Anti-Peripherin antibody [7C5] (AB254137)

Western blot - Anti-Peripherin antibody [7C5] (AB254137)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purity

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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