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AB4653

Anti-Peripherin antibody [8G2]

1

(1 Review)

|

(3 Publications)

Mouse Monoclonal Peripherin antibody. Suitable for WB, ICC/IF and reacts with Mouse, Rat, Human, Cow samples. Cited in 3 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Rat Prph.

View Alternative Names

Prph1, Prph, Peripherin

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-Peripherin antibody [8G2] (AB4653)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Peripherin antibody [8G2] (AB4653)

Immunofluorescent analysis of SH-SY5Y (human bone marrow neuroblastoma cell) cells (A) and PC-12 (rat adrenal gland pheochromocytoma cell) cells (B), stained with ab4653 at 1/500 dilution (Red) and costained with chicken polyclonal anti-vimentin antibody at 1/10,000 dilution (Green). Blue : DAPI staining of nuclear DNA.

Western blot - Anti-Peripherin antibody [8G2] (AB4653)
  • WB

Supplier Data

Western blot - Anti-Peripherin antibody [8G2] (AB4653)

The band at ~57kDa corresponds to the peripherin protein. Bands at 50 and 25kDa detected in the mouse spinal cord lysate, correspond to the heavy and light chains of mouse IgG.

All lanes:

Western blot - Anti-Peripherin antibody [8G2] (ab4653) at 1/500 dilution

Lane 2:

Mouse spinal cord lysate

Lane 3:

Rat spinal cord lysate

Lane 4:

Cow spinal cord lysate

Lane 5:

SH-SY5Y (human bone marrow neuroblastoma cell) whole cell lysate

Lane 6:

PC-12 (rat adrenal gland pheochromocytoma cell) whole cell lysate

Secondary

All lanes:

Anti-Mouse IgG

Predicted band size: 53 kDa

Observed band size: 25 kDa,50 kDa,57 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

8G2

Isotype

IgG1

Light chain type

kappa

Carrier free

No

Reacts with

Mouse, Rat, Cow, Human

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Full Length Protein corresponding to Rat Prph.

P21807

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "<p></p>" }, "Cow": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Purification notes
Purified antibody from TCS.
Storage buffer
Preservative: 0.032% Sodium azide Constituents: PBS, 49.98% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Peripherin also known as 8G2 or 7C5 is a protein expressed strongly in peripheral and certain central nervous system neurons. The mass of the peripherin protein is approximately 57 kDa. This intermediate filament protein structurally supports neurons aiding in their proper formation maintenance and regeneration. Peripherin localizes to the axons of mature neurons and serves important functions in the neuronal cytoskeleton and its stability.
Biological function summary

Peripherin impacts the nervous system's structure and dynamics. As part of the cytoskeleton it contributes to neurofilament networks which are essential for neuronal shape and mechanical integrity. Peripherin plays a role in neuronal differentiation and apoptosis. It operates independently but occasionally interacts with other cytoskeletal proteins like neurofilament light protein to perform its duties in neuronal cells.

Pathways

Peripherin is involved in processes associated with neuronal development and injury response. It features in neurogenesis pathways where it influences the growth and repair of axons. Peripherin interacts with proteins such as vimentin which helps manage cell dynamics and stress responses. The axonal transport pathway also utilizes peripherin to support axonal transport under stressful conditions ensuring cellular integrity and function.

Peripherin connects strongly to neurodegenerative diseases such as amyotrophic lateral sclerosis (ALS) and spinal muscular atrophy (SMA). Its overexpression or aggregation can create abnormal filamentous structures that contribute to motor neuron degeneration. In ALS peripherin may interact with TAR DNA-binding protein 43 (TDP-43) and superoxide dismutase 1 (SOD1) proteins known to be significant in the disease pathogenesis. Understanding peripherin’s role in these diseases aids in unraveling mechanisms underlying neuronal degeneration.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Class-III neuronal intermediate filament protein (PubMed : 2624740, PubMed : 3339087). My form an independent structural network without the involvement of other neurofilaments or may cooperate with the neuronal intermediate filament proteins NEFL, NEFH, NEFM and INA to form a filamentous network (By similarity). Assembly of the neuronal intermediate filaments may be regulated by RAB7A (By similarity). Plays a role in the development of unmyelinated sensory neurons (By similarity). May be involved in axon elongation and axon regeneration after injury (PubMed : 2585054, PubMed : 7530776). Inhibits neurite extension in type II spiral ganglion neurons in the cochlea (By similarity).
See full target information Prph
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

The Journal of biological chemistry 287:6002-13 PubMed22187436

2011

Up-regulation of Cavβ3 subunit in primary sensory neurons increases voltage-activated Ca2+ channel activity and nociceptive input in neuropathic pain.

Applications

IHC-FrFl

Species

Rat

Li Li,Xue-Hong Cao,Shao-Rui Chen,Hee-Dong Han,Gabriel Lopez-Berestein,Anil K Sood,Hui-Lin Pan

The Journal of comparative neurology 520:81-99 PubMed21674494

2011

A-kinase anchoring protein 150 expression in a specific subset of TRPV1- and CaV 1.2-positive nociceptive rat dorsal root ganglion neurons.

Applications

WB, ICC/IF

Species

Rat, Rat

Katherine E Brandao,Mark L Dell'Acqua,S Rock Levinson

Journal of neurocytology 27:69-84 PubMed9609398

1998

The intermediate filament protein peripherin is a marker for cerebellar climbing fibres.

Applications

Unspecified application

Species

Unspecified reactive species

L Errante,D Tang,M Gardon,G Sekerkova,E Mugnaini,G Shaw
View all publications
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Product promise

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