Rabbit Polyclonal Peripherin antibody. Suitable for WB, ICC/IF and reacts with Rat, Human samples. Cited in 41 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human PRPH.
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- The Journal of neuroscience : the official journal of the Society for Neuroscience 43:4174-41892023A New Mouse Model of Giant Axonal Neuropathy with Overt Phenotypes and Neurodegeneration Driven by Neurofilament Disorganization.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBanshi Nath,Jean-Pierre JulienPubMed 37137704
- Neurotoxicity research 41:41-562023Catalpol Mitigates Alzheimer's Disease Progression by Promoting the Expression of Neural Stem Cell Exosomes Released miR-138-5p.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShengxi Meng,Huize Chen,Chunjun Deng,Zeyu MengPubMed 36595161
- Stem cells international 2022:13209502022The Efficiency of Direct Maturation: the Comparison of Two hiPSC Differentiation Approaches into Motor Neurons.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCatherine Schaefers,Simone Rothmiller,Horst Thiermann,Theo Rein,Annette SchmidtPubMed 36530489
- Cell reports methods 2:1003412022Derivation of nociceptive sensory neurons from hiPSCs with early patterning and temporally controlled overexpression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWilliam Plumbly,Nikolaos Patikas,Sarah F Field,Stefanie Foskolou,Emmanouil MetzakopianPubMed 36452863
- Scientific reports 12:158692022Passage number affects differentiation of sensory neurons from human induced pluripotent stem cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesErica L Cantor,Fei Shen,Guanglong Jiang,Zhiyong Tan,Geneva M Cunningham,Xi Wu,Santosh Philips,Bryan P SchneiderPubMed 36151116
- Pain 164:43-582022ACVR1-activating mutation causes neuropathic pain and sensory neuron hyperexcitability in humans.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaobing Yu,Amy N Ton,Zejun Niu,Blanca M Morales,Jiadong Chen,Joao Braz,Michael H Lai,Emilie Barruet,Hongju Liu,Kin Cheung,Syed Ali,Tea Chan,Katherine Bigay,Jennifer Ho,Ina Nikolli,Steven Hansberry,Kelly Wentworth,Arnold Kriegstein,Allan Basbaum,Edward C HsiaoPubMed 35442931
- Nature communications 12:63072021A medullary centre for lapping in mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBowen Dempsey,Selvee Sungeelee,Phillip Bokiniec,Zoubida Chettouh,Séverine Diem,Sandra Autran,Evan R Harrell,James F A Poulet,Carmen Birchmeier,Harry Carey,Auguste Genovesio,Simon McMullan,Christo Goridis,Gilles Fortin,Jean-François BrunetPubMed 34728601
- Advanced science (Weinheim, Baden-Wurttemberg, Germany) 8:e21023542021NOCICEPTRA: Gene and microRNA Signatures and Their Trajectories Characterizing Human iPSC-Derived Nociceptor Maturation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMaximilian Zeidler,Kai K Kummer,Clemens L Schöpf,Theodora Kalpachidou,Georg Kern,M Zameel Cader,Michaela KressPubMed 34486248
- Neural regeneration research 16:2310-23152021Phenotypic and functional comparison of rat enteric neural crest-derived cells during fetal and early-postnatal stages.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDong-Hao Tian,Chuan-Hui Qin,Wen-Yao Xu,Wei-Kang Pan,Yu-Ying Zhao,Bai-Jun Zheng,Xin-Lin Chen,Yong Liu,Ya Gao,Hui YuPubMed 33818517
- Journal of bone oncology 26:1003302020The HMGB1/RAGE axis induces bone pain associated with colonization of 4T1 mouse breast cancer in bone.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTatsuo Okui,Masahiro Hiasa,Shoji Ryumon,Kisho Ono,Yuki Kunisada,Soichiro Ibaragi,Akira Sasaki,G David Roodman,Fletcher A White,Toshiyuki YonedaPubMed 33204606
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.01% Sodium azide
- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Full Length Protein corresponding to Human PRPH. Database link P41219
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Reactivity data
Select an application| WB | ICC/IF | |
|---|---|---|
| Human | Tested | Expected |
| Rat | Tested | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1/1000 - 1/2000 | Notes - |
Species Human | Dilution info 1/1000 - 1/2000 | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
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2 products for Alternative Product
Target data
Function
Class-III neuronal intermediate filament protein (By similarity). May form an independent structural network without the involvement of other neurofilaments or may cooperate with the neuronal intermediate filament proteins NEFL, NEFH, NEFM and INA to form a filamentous network (PubMed:15322088, PubMed:15446584). Assembly of the neuronal intermediate filaments may be regulated by RAB7A (By similarity). Plays a role in the development of unmyelinated sensory neurons (By similarity). May be involved in axon elongation and axon regeneration after injury (By similarity). Inhibits neurite extension in type II spiral ganglion neurons in the cochlea (By similarity).
Alternative names
NEF4, PRPH1, PRPH, Peripherin, Neurofilament 4
Recommended products
Rabbit Polyclonal Peripherin antibody. Suitable for WB, ICC/IF and reacts with Rat, Human samples. Cited in 41 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human PRPH.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.01% Sodium azide
- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Full Length Protein corresponding to Human PRPH. Database link P41219
- Purity
- Whole antiserum
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Peripherin also known as 8G2 or 7C5 is a protein expressed strongly in peripheral and certain central nervous system neurons. The mass of the peripherin protein is approximately 57 kDa. This intermediate filament protein structurally supports neurons aiding in their proper formation maintenance and regeneration. Peripherin localizes to the axons of mature neurons and serves important functions in the neuronal cytoskeleton and its stability.
- Biological function summary
Peripherin impacts the nervous system's structure and dynamics. As part of the cytoskeleton it contributes to neurofilament networks which are essential for neuronal shape and mechanical integrity. Peripherin plays a role in neuronal differentiation and apoptosis. It operates independently but occasionally interacts with other cytoskeletal proteins like neurofilament light protein to perform its duties in neuronal cells.
- Pathways
Peripherin is involved in processes associated with neuronal development and injury response. It features in neurogenesis pathways where it influences the growth and repair of axons. Peripherin interacts with proteins such as vimentin which helps manage cell dynamics and stress responses. The axonal transport pathway also utilizes peripherin to support axonal transport under stressful conditions ensuring cellular integrity and function.
- Associated diseases and disorders
Peripherin connects strongly to neurodegenerative diseases such as amyotrophic lateral sclerosis (ALS) and spinal muscular atrophy (SMA). Its overexpression or aggregation can create abnormal filamentous structures that contribute to motor neuron degeneration. In ALS peripherin may interact with TAR DNA-binding protein 43 (TDP-43) and superoxide dismutase 1 (SOD1) proteins known to be significant in the disease pathogenesis. Understanding peripherin’s role in these diseases aids in unraveling mechanisms underlying neuronal degeneration.
Product promise
Tested
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Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Immunocytochemistry/ Immunofluorescence - Anti-Peripherin antibody (ab4666)
Immunocytochemistry/Immunofluorescence analysis of mixed neuron/glia cultures from newborn rat brain labelling Peripherin (green) with ab4666 and Neurofilament heavy polypeptide (red) with Anti-Neurofilament heavy polypeptide antibody ab4680. A class of large neurons, like the one at the top right of this image, contain peripherin, while the majority of neurons and their processes contain Neurofilament heavy polypeptide and not peripherin. The blue channel reveals the localization of DNA.
Western blot - Anti-Peripherin antibody (ab4666)
All lanes: Western blot - Anti-Peripherin antibody (ab4666) at 1/1000 dilution
All lanes: Rat spinal cord homogenate
Predicted band size: 53 kDa
Observed band size: 57 kDa
Western blot - Anti-Peripherin antibody (ab4666)
Secondary antibody - goat anti-rabbit HRP preasorbed (Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ab97080)
All lanes: Western blot - Anti-Peripherin antibody (ab4666) at 1 µg/mL
All lanes: Western blot - Recombinant Human Peripherin protein (Recombinant Human Peripherin protein ab114335) at 0.01 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 53 kDa
Exposure time: 20s
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