AB4666

Anti-Peripherin antibody

Name: Anti-Peripherin antibody (ab4666) | Abcam
Brand: Abcam Limited
SKU: AB4666
Price: 515 USD
Availability: InStock
Rating: 5 (19 reviews)

(19 Reviews)

(50 Publications)

Anti-Peripherin antibody (ab4666) is a rabbit polyclonal antibody detecting Peripherin in Western Blot, ICC/IF. Suitable for Human, Rat.

- Over 40 publications
- Trusted since 2006

View Alternative Names

NEF4, PRPH1, PRPH, Peripherin, Neurofilament 4

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-Peripherin antibody (AB4666)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Peripherin antibody (AB4666)

Immunocytochemistry/Immunofluorescence analysis of mixed neuron/glia cultures from newborn rat brain labelling Peripherin (green) with ab4666 and Neurofilament heavy polypeptide (red) with ab4680. A class of large neurons, like the one at the top right of this image, contain peripherin, while the majority of neurons and their processes contain Neurofilament heavy polypeptide and not peripherin. The blue channel reveals the localization of DNA.

Supplier Data

Western blot - Anti-Peripherin antibody (AB4666)

All lanes:

Western blot - Anti-Peripherin antibody (ab4666) at 1/1000 dilution

All lanes:

Rat spinal cord homogenate

Predicted band size: 53 kDa

Observed band size: 57 kDa

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Unknown

Western blot - Anti-Peripherin antibody (AB4666)

Secondary antibody - goat anti-rabbit HRP preasorbed (ab97080)

All lanes:

Western blot - Anti-Peripherin antibody (ab4666) at 1 µg/mL

All lanes:

Western blot - Recombinant Human Peripherin protein (<a href='/en-us/products/proteins-peptides/recombinant-human-peripherin-protein-ab114335'>ab114335</a>) at 0.01 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

Predicted band size: 53 kDa

true

Exposure time: 20s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Rat, Human

Applications

ICC/IF, WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human PRPH.

P41219

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/2000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/2000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

What is this antibody validated in?
Anti-Peripherin antibody (ab4666) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Rat samples.

Trusted by the scientific community
Anti-Peripherin (ab4666) was first used in a scientific publication in 2006 and has been cited over 40 times in peer-reviewed journals.

Reviewed by scientists
Anti-Peripherin (ab4666) has over 15 independent reviews from customers.

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Properties and storage information

Form

Liquid

Purity

Whole antiserum

Storage buffer

Preservative: 0.01% Sodium azide

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Peripherin also known as 8G2 or 7C5 is a protein expressed strongly in peripheral and certain central nervous system neurons. The mass of the peripherin protein is approximately 57 kDa. This intermediate filament protein structurally supports neurons aiding in their proper formation maintenance and regeneration. Peripherin localizes to the axons of mature neurons and serves important functions in the neuronal cytoskeleton and its stability.

Biological function summary

Peripherin impacts the nervous system's structure and dynamics. As part of the cytoskeleton it contributes to neurofilament networks which are essential for neuronal shape and mechanical integrity. Peripherin plays a role in neuronal differentiation and apoptosis. It operates independently but occasionally interacts with other cytoskeletal proteins like neurofilament light protein to perform its duties in neuronal cells.

Pathways

Peripherin is involved in processes associated with neuronal development and injury response. It features in neurogenesis pathways where it influences the growth and repair of axons. Peripherin interacts with proteins such as vimentin which helps manage cell dynamics and stress responses. The axonal transport pathway also utilizes peripherin to support axonal transport under stressful conditions ensuring cellular integrity and function.

Peripherin connects strongly to neurodegenerative diseases such as amyotrophic lateral sclerosis (ALS) and spinal muscular atrophy (SMA). Its overexpression or aggregation can create abnormal filamentous structures that contribute to motor neuron degeneration. In ALS peripherin may interact with TAR DNA-binding protein 43 (TDP-43) and superoxide dismutase 1 (SOD1) proteins known to be significant in the disease pathogenesis. Understanding peripherin’s role in these diseases aids in unraveling mechanisms underlying neuronal degeneration.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Class-III neuronal intermediate filament protein (By similarity). May form an independent structural network without the involvement of other neurofilaments or may cooperate with the neuronal intermediate filament proteins NEFL, NEFH, NEFM and INA to form a filamentous network (PubMed : 15322088, PubMed : 15446584). Assembly of the neuronal intermediate filaments may be regulated by RAB7A (By similarity). Plays a role in the development of unmyelinated sensory neurons (By similarity). May be involved in axon elongation and axon regeneration after injury (By similarity). Inhibits neurite extension in type II spiral ganglion neurons in the cochlea (By similarity).

See full target information PRPH

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Publications (50)

Recent publications for all applications. Explore the full list and refine your search

Proceedings of the National Academy of Sciences of the United States of America 122:e2411174122 PubMed40663610

2025

A genetically defined pontine nucleus essential for ingestion in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Selvee Sungeelee,Caroline Mailhes-Hamon,Zoubida Chettouh,Phillip Bokiniec,Annaliese Eymael,Simon McMullan,Clément Léna,Bowen Dempsey,Jean-François Brunet

Journal of neurochemistry 169:e70004 PubMed39871624

2025

GABA Receptor Modulation of Membrane Excitability in Human Pluripotent Stem Cell-Derived Sensory Neurons by Baclofen and α-Conotoxin Vc1.1.

Applications

Unspecified application

Species

Unspecified reactive species

Mitchell St Clair-Glover,Arsalan Yousuf,Dominic Kaul,Mirella Dottori,David J Adams

Bone research 13:6 PubMed39800733

2025

Deep imaging of LepR stromal cells in optically cleared murine bone hemisections.

Applications

IHC

Species

Mouse

Yuehan Ni,Jiamiao Wu,Fengqi Liu,Yating Yi,Xiangjiao Meng,Xiang Gao,Luyi Xiao,Weiwei Zhou,Zexi Chen,Peng Chu,Dan Xing,Ye Yuan,Donghui Ding,Ge Shen,Min Yang,Ronjie Wu,Ling Wang,Luiza Martins Nascentes Melo,Sien Lin,Xiaoguang Cheng,Gang Li,Alpaslan Tasdogan,Jessalyn M Ubellacker,Hu Zhao,Shentong Fang,Bo Shen

Frontiers in surgery 11:1407948 PubMed39315293

2024

Paneth-like cells disruption and intestinal dysbiosis in the development of enterocolitis in an iatrogenic rectosigmoid hypoganglionosis rat model.

Applications

Unspecified application

Species

Unspecified reactive species

Iskandar Rahardjo Budianto,Kusmardi Kusmardi,Andi Muh Maulana,Somasundaram Arumugam,Rejina Afrin,Vivian Soetikno

British journal of pharmacology 181:2676-2696 PubMed38627101

2024

Calcium-sensing receptor regulates Kv7 channels via G protein signalling and modulates excitability of human induced pluripotent stem cell-derived nociceptive-like neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Nontawat Chuinsiri,Nannapat Siraboriphantakul,Luke Kendall,Polina Yarova,Christopher J Nile,Bing Song,Ilona Obara,Justin Durham,Vsevolod Telezhkin

The European journal of neuroscience 58:3206-3225 PubMed37574217

2023

Peripherin: A proposed biomarker of traumatic axonal injury triggered by mechanical force.

Applications

Unspecified application

Species

Unspecified reactive species

Tong Fang,Liang Yue,Zhu Longlong,Ma Longda,Huang Fang,Lv Yehui,Li Yang,Zhou Yiwu

The Journal of neuroscience : the official journal of the Society for Neuroscience 43:4174-4189 PubMed37137704

2023

A New Mouse Model of Giant Axonal Neuropathy with Overt Phenotypes and Neurodegeneration Driven by Neurofilament Disorganization.

Applications

Unspecified application

Species

Unspecified reactive species

Banshi Nath,Jean-Pierre Julien

Neurotoxicity research 41:41-56 PubMed36595161

2023

Catalpol Mitigates Alzheimer's Disease Progression by Promoting the Expression of Neural Stem Cell Exosomes Released miR-138-5p.

Applications

Unspecified application

Species

Unspecified reactive species

Shengxi Meng,Huize Chen,Chunjun Deng,Zeyu Meng

Stem cells international 2022:1320950 PubMed36530489

2022

The Efficiency of Direct Maturation: the Comparison of Two hiPSC Differentiation Approaches into Motor Neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Catherine Schaefers,Simone Rothmiller,Horst Thiermann,Theo Rein,Annette Schmidt

Cell reports methods 2:100341 PubMed36452863

2022

Derivation of nociceptive sensory neurons from hiPSCs with early patterning and temporally controlled overexpression.

Applications

Unspecified application

Species

Unspecified reactive species

William Plumbly,Nikolaos Patikas,Sarah F Field,Stefanie Foskolou,Emmanouil Metzakopian

View all publications

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