AB72422

Anti-Periplakin antibody

Name: Anti-Periplakin antibody (ab72422) | Abcam
Brand: Abcam Limited
SKU: AB72422
Price: 545 USD
Availability: InStock

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(2 Publications)

Rabbit Polyclonal Periplakin antibody. Suitable for IP, WB and reacts with Human, Mouse, Rat samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human PPL.

View Alternative Names

KIAA0568, PPL, Periplakin, 190 kDa paraneoplastic pemphigus antigen, 195 kDa cornified envelope precursor protein

3 Images

Unknown

Western blot - Anti-Periplakin antibody (AB72422)

All lanes:

Western blot - Anti-Periplakin antibody (ab72422) at 0.04 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 5 µg

Predicted band size: 205 kDa

Observed band size: 205 kDa,55 kDa

false

Exposure time: 10s

Unknown

Immunoprecipitation - Anti-Periplakin antibody (AB72422)

Detection of Periplakin by Western Blot of Immunprecipitate.
ab72422 at 1µg/ml staining Periplakin in HeLa whole cell lysate immunoprecipitated using ab72422 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection : Chemiluminescence with exposure time of 10 seconds.

All lanes:

Immunoprecipitation - Anti-Periplakin antibody (ab72422)

Predicted band size: 205 kDa

false

Supplier Data

Western blot - Anti-Periplakin antibody (AB72422)

All lanes:

Western blot - Anti-Periplakin antibody (ab72422) at 1/2000 dilution

Lane 1:

NIH 3T3 Whole cell lysate at 50 µg

Lane 2:

TCMK-1 Whole cell lysate at 50 µg

Lane 3:

4T1 Whole cell lysate at 50 µg

Lane 4:

CT26.WT Whole cell lysate at 50 µg

Lane 5:

C6 Whole cell lysate at 50 µg

Secondary

All lanes:

Goat anti-Rabbit Light Chain HRP Conjugate

Predicted band size: 205 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

IP, WB

applications

Immunogen

Synthetic Peptide within Human PPL. The exact immunogen used to generate this antibody is proprietary information.

O60437

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Amphibian": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Guinea pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Medaka fish": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Xenopus laevis": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab72422 was affinity purified using an epitope specific to Periplakin immobilized on solid support.

Storage buffer

pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Periplakin also known as PPL is a protein that plays an important role in cell adhesion and maintaining cellular structures. It is part of the plakin protein family distinguished by their ability to link cytoskeletal elements in cells. The molecular weight of Periplakin is approximately 195 kDa. Expression of Periplakin occurs mainly in epithelial tissues where it contributes to the formation of desmosomes and other adhesion complexes important for tissue integrity.

Biological function summary

Periplakin participates in the structural organization of epithelial cells. It associates with other proteins to form cytoskeletal linker complexes that enhance cellular stability. As a component of the cornified envelope Periplakin interacts with envoplakin and involucrin. These interactions modulate the cross-linking within the cell envelope impacting the barrier function of the skin and mucous membranes.

Pathways

Periplakin involvement influences epithelial tissue differentiation and stability. It acts within the desmosome assembly pathway impacting cell-cell adhesion processes critical for tissue architecture. Within this pathway Periplakin associates with desmoplakin and envoplakin enhancing cell cohesion and resistance to mechanical stress.

Periplakin has been associated with skin conditions such as pemphigus and certain types of cancers. In pemphigus an autoimmune disease antibodies target desmosome components leading to weakened cell adhesion. These events can disrupt Periplakin interactions affecting cellular stability. In cancer altered expression of Periplakin might impact cellular cohesion and migration. Such alterations in association with proteins like envoplakin and involucrin may contribute to disease progression.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Component of the cornified envelope of keratinocytes. May link the cornified envelope to desmosomes and intermediate filaments. May act as a localization signal in PKB/AKT-mediated signaling.

See full target information PPL

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cell reports. Medicine 1:100129 PubMed33294854

2020

Topical Aminosalicylic Acid Improves Keratinocyte Differentiation in an Inducible Mouse Model of Harlequin Ichthyosis.

Applications

Unspecified application

Species

Unspecified reactive species

Denny L Cottle,Gloria M A Ursino,Lynelle K Jones,Ming Shen Tham,Allara K Zylberberg,Ian M Smyth

Cell death & disease 9:1072 PubMed30341279

2018

Loss of GRHL3 leads to TARC/CCL17-mediated keratinocyte proliferation in the epidermis.

Applications

Unspecified application

Species

Unspecified reactive species

Stephen J Goldie,Denny L Cottle,Fiona H Tan,Suraya Roslan,Seema Srivastava,Rhys Brady,Darren D Partridge,Alana Auden,Ian M Smyth,Stephen M Jane,Sebastian Dworkin,Charbel Darido

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Periplakin antibody

Western blot - Anti-Periplakin antibody (AB72422)

Immunoprecipitation - Anti-Periplakin antibody (AB72422)

Western blot - Anti-Periplakin antibody (AB72422)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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