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Proteins and peptidesAnti-Ly6g antibody [1A8] - mouse IgG2c (Chimeric)
Low endotoxin, Azide free.
Our first-to-market chimera with mouse IgG2c backbone, this functional antibody specifically depletes neutrophils in vivo for up to 72h.
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Rabbit Recombinant Monoclonal Periplakin antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 5 publications.
IgG
Rabbit
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA
Liquid
Monoclonal
IHC-P | ICC/IF | IP | Flow Cyt | WB | |
---|---|---|---|---|---|
Human | Tested | Not recommended | Not recommended | Not recommended | Tested |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/50 - 1/100 | Notes (Heat to 98°C, allow to cool for 10-20 minutes) Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/1000 - 1/10000 | Notes - |
Component of the cornified envelope of keratinocytes. May link the cornified envelope to desmosomes and intermediate filaments. May act as a localization signal in PKB/AKT-mediated signaling.
Periplakin, 190 kDa paraneoplastic pemphigus antigen, 195 kDa cornified envelope precursor protein, PPL, KIAA0568
Rabbit Recombinant Monoclonal Periplakin antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 5 publications.
Periplakin, 190 kDa paraneoplastic pemphigus antigen, 195 kDa cornified envelope precursor protein, PPL, KIAA0568
IgG
Rabbit
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA
Liquid
Monoclonal
EPR8296
Affinity purification Protein A
1.82 x 10-11 M
Blue Ice
-20°C
Stable for 12 months at -20°C
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
Periplakin participates in the structural organization of epithelial cells. It associates with other proteins to form cytoskeletal linker complexes that enhance cellular stability. As a component of the cornified envelope Periplakin interacts with envoplakin and involucrin. These interactions modulate the cross-linking within the cell envelope impacting the barrier function of the skin and mucous membranes.
Periplakin also known as PPL is a protein that plays an important role in cell adhesion and maintaining cellular structures. It is part of the plakin protein family distinguished by their ability to link cytoskeletal elements in cells. The molecular weight of Periplakin is approximately 195 kDa. Expression of Periplakin occurs mainly in epithelial tissues where it contributes to the formation of desmosomes and other adhesion complexes important for tissue integrity.
Periplakin involvement influences epithelial tissue differentiation and stability. It acts within the desmosome assembly pathway impacting cell-cell adhesion processes critical for tissue architecture. Within this pathway Periplakin associates with desmoplakin and envoplakin enhancing cell cohesion and resistance to mechanical stress.
Periplakin has been associated with skin conditions such as pemphigus and certain types of cancers. In pemphigus an autoimmune disease antibodies target desmosome components leading to weakened cell adhesion. These events can disrupt Periplakin interactions affecting cellular stability. In cancer altered expression of Periplakin might impact cellular cohesion and migration. Such alterations in association with proteins like envoplakin and involucrin may contribute to disease progression.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
All lanes: Western blot - Anti-Periplakin antibody [EPR8296] (AB131269) at 1/1000 dilution
Lane 1: HACAT lysate at 10 µg
Lane 2: Human skin lysate at 10 µg
All lanes: Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 205 kDa
Immunohistochemical analysis of paraffin embedded Human skin tissue labelling Periplakin with ab131269 at 1/50 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com