AB131269

Anti-Periplakin antibody [EPR8296]

RabMAb
Recombinant
What is this?

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(7 Publications)

Rabbit Recombinant Monoclonal Periplakin antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 7 publications.

View Alternative Names

KIAA0568, PPL, Periplakin, 190 kDa paraneoplastic pemphigus antigen, 195 kDa cornified envelope precursor protein

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Periplakin antibody [EPR8296] (AB131269)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Periplakin antibody [EPR8296] (AB131269)

Immunohistochemical analysis of paraffin embedded Human skin tissue labelling Periplakin with ab131269 at 1/50 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Unknown

Western blot - Anti-Periplakin antibody [EPR8296] (AB131269)

All lanes:

Western blot - Anti-Periplakin antibody [EPR8296] (ab131269) at 1/1000 dilution

Lane 1:

HACAT lysate at 10 µg

Lane 2:

Human skin lysate at 10 µg

Secondary

All lanes:

Goat anti-Rabbit HRP at 1/2000 dilution

Predicted band size: 205 kDa

false

OI-RD Scanning - Anti-Periplakin antibody [EPR8296] (AB131269)

OI-RD Scanning

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OI-RD Scanning - Anti-Periplakin antibody [EPR8296] (AB131269)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Carrier free

Anti-Periplakin antibody [EPR8296] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR8296

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "(Heat to 98°C, allow to cool for 10-20 minutes)", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "" } } }

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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Periplakin also known as PPL is a protein that plays an important role in cell adhesion and maintaining cellular structures. It is part of the plakin protein family distinguished by their ability to link cytoskeletal elements in cells. The molecular weight of Periplakin is approximately 195 kDa. Expression of Periplakin occurs mainly in epithelial tissues where it contributes to the formation of desmosomes and other adhesion complexes important for tissue integrity.

Biological function summary

Periplakin participates in the structural organization of epithelial cells. It associates with other proteins to form cytoskeletal linker complexes that enhance cellular stability. As a component of the cornified envelope Periplakin interacts with envoplakin and involucrin. These interactions modulate the cross-linking within the cell envelope impacting the barrier function of the skin and mucous membranes.

Pathways

Periplakin involvement influences epithelial tissue differentiation and stability. It acts within the desmosome assembly pathway impacting cell-cell adhesion processes critical for tissue architecture. Within this pathway Periplakin associates with desmoplakin and envoplakin enhancing cell cohesion and resistance to mechanical stress.

Periplakin has been associated with skin conditions such as pemphigus and certain types of cancers. In pemphigus an autoimmune disease antibodies target desmosome components leading to weakened cell adhesion. These events can disrupt Periplakin interactions affecting cellular stability. In cancer altered expression of Periplakin might impact cellular cohesion and migration. Such alterations in association with proteins like envoplakin and involucrin may contribute to disease progression.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Component of the cornified envelope of keratinocytes. May link the cornified envelope to desmosomes and intermediate filaments. May act as a localization signal in PKB/AKT-mediated signaling.

See full target information PPL

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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Cells 14: PubMed40710298

2025

Bioengineering a Human Dermal Equivalent Using Induced Pluripotent Stem Cell-Derived Fibroblasts to Support the Formation of a Full-Thickness Skin Construct.

Applications

Unspecified application

Species

Unspecified reactive species

Lucy Smith,David Bunton,Michael Finch,Stefan Przyborski

Journal of the European Academy of Dermatology and Venereology : JEADV 37:605-614 PubMed36367625

2022

An open source pipeline for quantitative immunohistochemistry image analysis of inflammatory skin disease using artificial intelligence.

Applications

Unspecified application

Species

Unspecified reactive species

Yuchun Ding,Gaurav Dhawan,Claire Jones,Thomas Ness,Esme Nichols,Natalio Krasnogor,Nick J Reynolds

Translational cancer research 11:1372-1385 PubMed35706781

2022

Mechanism of periplakin on ovarian cancer cell phenotype and its influence on prognosis.

Applications

Unspecified application

Species

Unspecified reactive species

Lijing Liu,Lin Ding,Lanli Zhang,Hongtao Zhang,Yu Zhang,Dan Liu

Discover. Oncology 13:35 PubMed35612641

2022

Prognostic implications of PPL expression in ovarian cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Tian Hua,Bei-Bei Zhao,Shao-Bei Fan,Cai-Fen Zhao,Yun-Hong Kong,Rui-Qing Tian,Bao-Ying Zhang

Nature communications 12:6795 PubMed34815391

2021

Desmoplakin and periplakin genetically and functionally contribute to eosinophilic esophagitis.

Applications

Unspecified application

Species

Unspecified reactive species

Tetsuo Shoda,Kenneth M Kaufman,Ting Wen,Julie M Caldwell,Garrett A Osswald,Pathre Purnima,Nives Zimmermann,Margaret H Collins,Kira Rehn,Heather Foote,Michael D Eby,Wenying Zhang,Netali Ben-Baruch Morgenstern,Adina Y Ballaban,Jeff E Habel,Leah C Kottyan,J Pablo Abonia,Vincent A Mukkada,Philip E Putnam,Lisa J Martin,Marc E Rothenberg

Plastic and reconstructive surgery. Global open 7:e2339 PubMed31592376

2019

Skin Protein Profile after Major Weight Loss and Its Role in Body Contouring Surgery.

Applications

Unspecified application

Species

Unspecified reactive species

José Renato B Gallo,Larissa B Maschio-Signorini,Celso R B Cabral,Debora A P de Campos Zuccari,Maurício L Nogueira,Antônio R Bozola,Patricia M Cury,Alessandra Vidotto

Scientific reports 9:2357 PubMed30787334

2019

The 4717C > G polymorphism in periplakin modulates sensitivity to EGFR inhibitors.

Applications

Unspecified application

Species

Unspecified reactive species

Hui Mei Lee,Gregory Michael Kelly,Nur Syafinaz Zainal,Pei San Yee,Muhammad Zaki Hidayatullah Fadlullah,Bernard Kok Bang Lee,Chai Phei Gan,Vyomesh Patel,Sok Ching Cheong

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Periplakin antibody [EPR8296]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Periplakin antibody [EPR8296] (AB131269)

Western blot - Anti-Periplakin antibody [EPR8296] (AB131269)

OI-RD Scanning - Anti-Periplakin antibody [EPR8296] (AB131269)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

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Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (7)

Product promise