Rabbit Recombinant Monoclonal Periplakin antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human samples.
Images
![Western blot - Anti-Periplakin antibody [EPR8296] - BSA and Azide free (AB248397), expandable thumbnail](https://content.abcam.com/products/images/periplakin-antibody-epr8296-bsa-and-azide-free-ab248397--western-blot-img129720.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Periplakin antibody [EPR8296] - BSA and Azide free (AB248397)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Periplakin antibody [EPR8296] - BSA and Azide free (AB248397), expandable thumbnail](https://content.abcam.com/products/images/periplakin-antibody-epr8296-bsa-and-azide-free-ab248397--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img48918.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Periplakin antibody [EPR8296] - BSA and Azide free (AB248397)")
![OI-RD Scanning - Anti-Periplakin antibody [EPR8296] - BSA and Azide free (AB248397), expandable thumbnail](https://content.abcam.com/products/images/periplakin-antibody-epr8296-bsa-and-azide-free-ab248397--oi-rd-scanning-img83897.jpg/_jcr_content/renditions/webp-475.webp "OI-RD Scanning - Anti-Periplakin antibody [EPR8296] - BSA and Azide free (AB248397)")
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ICC/IF | Flow Cyt | IHC-P | IP | WB | |
|---|---|---|---|---|---|
| Human | Not recommended | Not recommended | Tested | Not recommended | Tested |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes For antigen retrieval, heat up to 98 degree C, below boiling, and then let cool for 10-20 minutes. Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 products for Alternative Version
Target data
Function
Component of the cornified envelope of keratinocytes. May link the cornified envelope to desmosomes and intermediate filaments. May act as a localization signal in PKB/AKT-mediated signaling.
Alternative names
Periplakin, 190 kDa paraneoplastic pemphigus antigen, 195 kDa cornified envelope precursor protein, PPL, KIAA0568
Recommended products
Rabbit Recombinant Monoclonal Periplakin antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human samples.
Alternative names
Periplakin, 190 kDa paraneoplastic pemphigus antigen, 195 kDa cornified envelope precursor protein, PPL, KIAA0568
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
Yes
- Clone number
EPR8296
- Purification technique
Affinity purification Protein A
- Dissociation constant
1.82 x 10-11 M
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
- Storage information
Do Not Freeze
Notes
ab248397 is the carrier-free version of Anti-Periplakin antibody [EPR8296] ab131269.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- Activity summary
Periplakin also known as PPL is a protein that plays an important role in cell adhesion and maintaining cellular structures. It is part of the plakin protein family distinguished by their ability to link cytoskeletal elements in cells. The molecular weight of Periplakin is approximately 195 kDa. Expression of Periplakin occurs mainly in epithelial tissues where it contributes to the formation of desmosomes and other adhesion complexes important for tissue integrity.
- Biological function summary
Periplakin participates in the structural organization of epithelial cells. It associates with other proteins to form cytoskeletal linker complexes that enhance cellular stability. As a component of the cornified envelope Periplakin interacts with envoplakin and involucrin. These interactions modulate the cross-linking within the cell envelope impacting the barrier function of the skin and mucous membranes.
- Pathways
Periplakin involvement influences epithelial tissue differentiation and stability. It acts within the desmosome assembly pathway impacting cell-cell adhesion processes critical for tissue architecture. Within this pathway Periplakin associates with desmoplakin and envoplakin enhancing cell cohesion and resistance to mechanical stress.
- Associated diseases and disorders
Periplakin has been associated with skin conditions such as pemphigus and certain types of cancers. In pemphigus an autoimmune disease antibodies target desmosome components leading to weakened cell adhesion. These events can disrupt Periplakin interactions affecting cellular stability. In cancer altered expression of Periplakin might impact cellular cohesion and migration. Such alterations in association with proteins like envoplakin and involucrin may contribute to disease progression.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Western blot - Anti-Periplakin antibody [EPR8296] - BSA and Azide free (ab248397), expandable thumbnail](https://content.abcam.com/products/images/periplakin-antibody-epr8296-bsa-and-azide-free-ab248397--western-blot-img129720.jpg "Western blot - Anti-Periplakin antibody [EPR8296] - BSA and Azide free (ab248397)")
Western blot - Anti-Periplakin antibody [EPR8296] - BSA and Azide free (ab248397)
This data was developed using Anti-Periplakin antibody [EPR8296] ab131269, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-Periplakin antibody [EPR8296] (Anti-Periplakin antibody [EPR8296] ab131269) at 1/1000 dilution
Lane 1: HACAT lysate at 10 µg
Lane 2: Human skin lysate at 10 µg
SecondaryAll lanes: Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 205 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Periplakin antibody [EPR8296] - BSA and Azide free (ab248397), expandable thumbnail](https://content.abcam.com/products/images/periplakin-antibody-epr8296-bsa-and-azide-free-ab248397--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img48918.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Periplakin antibody [EPR8296] - BSA and Azide free (ab248397)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Periplakin antibody [EPR8296] - BSA and Azide free (ab248397)
This data was developed using Anti-Periplakin antibody [EPR8296] ab131269, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded Human skin tissue labelling Periplakin with Anti-Periplakin antibody [EPR8296] ab131269 at 1/50 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
![OI-RD Scanning - Anti-Periplakin antibody [EPR8296] - BSA and Azide free (ab248397), expandable thumbnail](https://content.abcam.com/products/images/periplakin-antibody-epr8296-bsa-and-azide-free-ab248397--oi-rd-scanning-img83897.jpg "OI-RD Scanning - Anti-Periplakin antibody [EPR8296] - BSA and Azide free (ab248397)")
OI-RD Scanning - Anti-Periplakin antibody [EPR8296] - BSA and Azide free (ab248397)
We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com