Anti-PERK antibody

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PERK antibody (AB79483)

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue, stained with ab79483 at 1/50-1/100 dilution, in the absence and presence of the immunising peptide

• IHC-P

AbReview40562****

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PERK antibody (AB79483)

IHC-P image of PERK staining on human cortical sections using ab79483 (1 : 500). The tissue was fixed in formaldehyde and the sections were then subjected to heat mediated antigen retrieval using citric acid and permeabilized using Triton-X. The sections were them blocked using 2% BSA for 1 hour at 20°C. ab79483 was diluted 1 : 500 and incubated with the sections for 18 hours at 20°C. The secondary antibody used was HRP conjugated Goat polyclonal to Rabbit IgG (1 : 500)

This image is courtesy of an anonymous abreview.

• WB

Unknown

Western blot - Anti-PERK antibody (AB79483)

All lanes:

Western blot - Anti-PERK antibody (ab79483) at 1/500 dilution

Lane 1:

extracts from MCF-7 cells at 30 µg

Lane 2:

extracts from MCF-7 cells at 30 µg with immunising peptide

Predicted band size: 125 kDa

Observed band size: 125 kDa

false

• WB

CiteAb

Western blot - Anti-PERK antibody (AB79483)

PERK western blot using anti-PERK antibody ab79483. Publication image and figure legend from Li, D., Wang, W. J., et al., 2019, Cell Death Dis, PubMed 31582720.

ab79483 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab79483 please see the product overview.

125I induces upregulation of PERK-eIF2a-ATF4-CHOP pathway to promote apoptosis.a To explore the possible apoptosis-related pathways induced by 125I, iTRAQ was performed in HepG2 cells. The results of the signal pathway enrichment analysis of differentially expressed proteins reveals that the eIF2 signaling pathway is significantly different between the control HepG2 cells and 125I-treated HepG2 cells. b, c After HepG2 cells were treated with different doses of 125I, the proteins and mRNA levels of the PERK-eIF2-ATF4-CHOP pathway were upregulated in a dose-dependent manner, as detected by WB (b) and qPCR (c). d The mRNA levels consistently increased in animal models in a dose-dependent manner, after 125I irradiation. Total RNA was isolated from mouse tumors and the pathway-related genes were seen to be upregulated at the mRNA level, as detected by qPCR. e, f The 125I-induced apoptosis and anti-proliferation effect were compromised by PERK-RNAi. After HepG2 cells transfected with Control-RNAi or PERK-RNAi were treated by 125I, the apoptosis and anti-proliferation effect were detected by cell proliferation assay and Annexin V–FITC/PI assay. The PERK-RNAi abrogated 125I-induced apoptosis and anti-proliferation effect. All the experiments were performed in triplicate and the data are presented as the mean ± SD. The t-test was used for data analysis. *p < 0.05, **p < 0.01

false