Rabbit Recombinant Monoclonal PERK antibody. Suitable for WB and reacts with Mouse, Rat, Human samples. Cited in 45 publications.
IgG
Rabbit
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Liquid
Monoclonal
IP | Flow Cyt | WB | IHC-P | ICC/IF | |
---|---|---|---|---|---|
Human | Not recommended | Not recommended | Tested | Not recommended | Not recommended |
Mouse | Not recommended | Not recommended | Tested | Not recommended | Not recommended |
Rat | Not recommended | Not recommended | Tested | Not recommended | Not recommended |
Species | Dilution info | Notes |
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Species Mouse, Rat, Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
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Species Mouse, Rat, Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
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Species Mouse | Dilution info 1/1000 | Notes - |
Species Rat | Dilution info 1/1000 | Notes - |
Species Human | Dilution info 1/1000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
Metabolic-stress sensing protein kinase that phosphorylates the alpha subunit of eukaryotic translation initiation factor 2 (EIF2S1/eIF-2-alpha) in response to various stress, such as unfolded protein response (UPR) (PubMed:10026192, PubMed:10677345, PubMed:11907036, PubMed:12086964, PubMed:25925385, PubMed:31023583). Key effector of the integrated stress response (ISR) to unfolded proteins: EIF2AK3/PERK specifically recognizes and binds misfolded proteins, leading to its activation and EIF2S1/eIF-2-alpha phosphorylation (PubMed:10677345, PubMed:27917829, PubMed:31023583). EIF2S1/eIF-2-alpha phosphorylation in response to stress converts EIF2S1/eIF-2-alpha in a global protein synthesis inhibitor, leading to a global attenuation of cap-dependent translation, while concomitantly initiating the preferential translation of ISR-specific mRNAs, such as the transcriptional activators ATF4 and QRICH1, and hence allowing ATF4- and QRICH1-mediated reprogramming (PubMed:10026192, PubMed:10677345, PubMed:31023583, PubMed:33384352). The EIF2AK3/PERK-mediated unfolded protein response increases mitochondrial oxidative phosphorylation by promoting ATF4-mediated expression of COX7A2L/SCAF1, thereby increasing formation of respiratory chain supercomplexes (PubMed:31023583). In contrast to most subcellular compartments, mitochondria are protected from the EIF2AK3/PERK-mediated unfolded protein response due to EIF2AK3/PERK inhibition by ATAD3A at mitochondria-endoplasmic reticulum contact sites (PubMed:39116259). In addition to EIF2S1/eIF-2-alpha, also phosphorylates NFE2L2/NRF2 in response to stress, promoting release of NFE2L2/NRF2 from the BCR(KEAP1) complex, leading to nuclear accumulation and activation of NFE2L2/NRF2 (By similarity). Serves as a critical effector of unfolded protein response (UPR)-induced G1 growth arrest due to the loss of cyclin-D1 (CCND1) (By similarity). Involved in control of mitochondrial morphology and function (By similarity).
PEK, PERK, PERK, PEK, EIF2AK3, Eukaryotic translation initiation factor 2-alpha kinase 3, PRKR-like endoplasmic reticulum kinase, Pancreatic eIF2-alpha kinase, Protein tyrosine kinase EIF2AK3, HsPEK
Rabbit Recombinant Monoclonal PERK antibody. Suitable for WB and reacts with Mouse, Rat, Human samples. Cited in 45 publications.
IgG
Rabbit
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Liquid
Monoclonal
EPR19876-294
Affinity purification Protein A
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
This supplementary information is collated from multiple sources and compiled automatically.
PERK also known as EIF2AK3 is a protein kinase involved in the unfolded protein response (UPR). Researchers commonly identify it by its phosphorylation state in western blots recognizable as phospho-PERK or p-PERK. The molecular weight of PERK is approximately 125 kDa. It is predominantly expressed in the endoplasmic reticulum (ER) of cells acting as a sensor for misfolded proteins. PERK acts mechanistically by phosphorylating the translation initiation factor eIF2α reducing overall protein synthesis and alleviating ER stress.
PERK plays an important role in maintaining cellular homeostasis during stress conditions. It forms part of a larger complex that regulates the adaptive response to ER stress. Upon activation PERK arrests general protein translation while allowing for selective translation of stress response proteins. This mechanism prevents the accumulation of unfolded proteins and assists in the survival of cells under adverse conditions. PERK also contributes to the regulation of oxidative stress and cell apoptosis adding further layers to its biological significance.
PERK's activity intersects with the integrated stress response and UPR pathways. It shares a close functional relationship with proteins like ATF4 which is synthesized upon PERK activation and is important for the transcription of genes related to stress adaptation. The UPR pathway coordinates with the IRE1 and ATF6 pathways forming an important network for managing ER stress. These pathways collectively ensure cellular resilience adjusting metabolic demands and promoting cellular recovery.
PERK's malfunction can contribute to conditions such as diabetes and neurodegenerative diseases like Alzheimer's. In diabetes improper regulation of PERK affects insulin synthesis and secretion leading to pancreatic β-cell dysfunction. Alzheimer’s disease shows links with heightened ER stress and dysregulation of the PERK-eIF2α signaling pathway potentially contributing to neuronal death. PERK dysfunction can also associate with other proteins such as CHOP which mediates apoptosis during prolonged ER stress exacerbating these conditions.
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This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
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Exposure time: Lanes 1-2: 37 seconds; Lanes 3-6: 3 minutes.
Blocking/Dilution buffer: 5% NFDM/TBST.
This antibody also recognizes unidentifiable proteins below 37 kDa.
All lanes: Western blot - Anti-PERK antibody [EPR19876-294] (ab229912) at 1/1000 dilution
Lane 1: RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate at 20 µg
Lane 2: NIH/3T3 (mouse embyro fibroblast cell line) whole cell lysate at 20 µg
Lane 3: PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell lysate at 20 µg
Lane 4: Mouse brain lysate at 20 µg
Lane 5: Rat brain lysate at 20 µg
Lane 6: Rat liver lysate at 20 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 125 kDa
Observed band size: 150 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
ab229912 was shown to specifically react with PERK in wild-type HAP1 cells as signal was lost in PERK knockout cells. Wild-type and PERK knockout samples were subjected to SDS-PAGE. ab229912 and Anti-GAPDH antibody [EPR16891] - Loading Control ab181602 (Rabbit anti-GAPDH loading control) were incubated 1 hour at room temperature at 1/1000 dilution and 1/200,000 dilution respectively. Blots were developed with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) secondary antibody at 1/100,000 dilution for 1 hour at room temperature before imaging. The blot was developed on a BIO-RAD® ChemiDoc™ MP instrument using the ECL technique.
This antibody also recognizes unidentifiable proteins below 75 kDa.
All lanes: Western blot - Anti-PERK antibody [EPR19876-294] (ab229912) at 1/1000 dilution
Lane 1: Wild-type HAP1 whole cell lysate at 20 µg
Lane 2: PERK knockout HAP1 whole cell lysate at 20 µg
Lane 3: U-87 MG (human glioblastoma-astrocytoma epithelial cell line) whole cell lysate at 20 µg
Lane 4: HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg
Lane 5: HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 20 µg
Lane 6: MCF7 (human breast adenocarcinoma cell line) whole cell lysate at 20 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 125 kDa
Observed band size: 150 kDa
Exposure time: 125s
Blocking/Dilution buffer: 5% NFDM/TBST.
ab229912 was shown to specifically react with PERK in wild-type HAP1 cells as signal was lost in PERK knockout cells. Wild-type and PERK knockout samples were subjected to SDS-PAGE. ab229912 and Anti-GAPDH antibody [EPR16891] - Loading Control ab181602 (Rabbit anti-GAPDH loading control) were incubated 1 hour at room temperature at 1/1000 dilution and 1/200,000 dilution respectively. Blots were developed with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) secondary antibody at 1/100,000 dilution for 1 hour at room temperature before imaging. The blot was developed on a BIO-RAD® ChemiDoc™ MP instrument using the ECL technique.
This antibody also recognizes unidentifiable proteins below 75 kDa.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab229912).
Western blot: Anti-EIF2AK3 antibody [EPR19876-294] (ab229912) staining at 1/1000 dilution, shown in green; Mouse anti-CANX [CANX/1543] (Anti-Calnexin antibody [CANX/1543] ab238078) loading control staining at 1/20000 dilution, shown in magenta. In Western blot, ab229912 was shown to bind specifically to EIF2AK3. A band was observed at 145 kDa in wild-type A549 cell lysates with no signal observed at this size in EIF2AK3 knockout cell line. To generate this image, wild-type and EIF2AK3 knockout A549 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.
All lanes: Western blot - Anti-PERK antibody [EPR19876-294] (ab229912) at 1/1000 dilution
Lane 1: Wild-type A549 cell lysate at 20 µg
Lane 2: EIF2AK3 knockout A549 cell lysate at 20 µg
Lane 3: MCF7 cell lysate at 20 µg
Lane 4: Human eye cell lysate at 20 µg
All lanes: Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution
Performed under reducing conditions.
Observed band size: 145 kDa
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