Knockout Tested Rabbit Polyclonal Peroxiredoxin 1/PAG antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human, African green monkey samples. Cited in 36 publications. Immunogen corresponding to Synthetic Peptide within Human PRDX1 aa 100-150.
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- Cell death discovery 9:4252023Peroxiredoxin 1 regulates crosstalk between pyroptosis and autophagy in oral squamous cell carcinoma leading to a potential pro-survival.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMeilin Ye,Ting Liu,Shanshan Liu,Rong Tang,Hongrui Liu,Fan Zhang,Shenglei Luo,Minqi LiPubMed 38007535
- Antioxidants (Basel, Switzerland) 12:2023Peroxiredoxin V Protects against UVB-Induced Damage of Keratinocytes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSin Ri Kim,Ji Won Park,Byung-Hoon Lee,Kyung Min Lim,Tong-Shin ChangPubMed 37507973
- Disease markers 2022:96510922022Death-Associated Protein Kinase 1 (DAPK1) Protects against Myocardial Injury Induced by Myocardial Infarction in Rats via Inhibition of Inflammation and Oxidative Stress.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJun Zhang,Jing Zhang,Bo Zhou,Xiaojing Jiang,Yanrong Tang,Zhenzhen ZhangPubMed 35082934
- Antioxidants (Basel, Switzerland) 10:2021The Human 2-Cys Peroxiredoxins form Widespread, Cysteine-Dependent- and Isoform-Specific Protein-Protein Interactions.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLoes van Dam,Marc Pagès-Gallego,Paulien E Polderman,Robert M van Es,Boudewijn M T Burgering,Harmjan R Vos,Tobias B DansenPubMed 33923941
- International journal of molecular sciences 22:2021Oxidative Stress-Induced Sirtuin1 Downregulation Correlates to HIF-1α, GLUT-1, and VEGF-A Upregulation in Th1 Autoimmune Hashimoto's Thyroiditis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMichaël Hepp,Alexis Werion,Axel De Greef,Christine de Ville de Goyet,Marc de Bournonville,Catherine Behets,Benoit Lengelé,Chantal Daumerie,Michel Mourad,Marian Ludgate,Marie-Christine Many,Virginie Joris,Julie CrapsPubMed 33916948
- Cells 10:2021Placental Glycoredox Dysregulation Associated with Disease Progression in an Animal Model of Superimposed Preeclampsia.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSandra M Blois,Paula D Prince,Sophia Borowski,Monica Galleano,Gabriela BarrientosPubMed 33916770
- The Journal of international medical research 49:3000605209863132021Silencing of peroxiredoxin 1 expression ameliorates ulcerative colitis in a rat model.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNa Wu,Xinchong Du,Zhao Peng,Zetian Zhang,Lijun Cui,Duo Li,Rui Wang,Maoyuan MaPubMed 33682513
- Molecular medicine reports 22:4734-47422020Inflammatory response and oxidative stress attenuated by sulfiredoxin‑1 in neuron‑like cells depends on nuclear factor erythroid‑2‑related factor 2.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhiliang Wu et. al.PubMed 33173963
- Biochemical and biophysical research communications 527:861-8652020Peroxiredoxin-1 aggravates lipopolysaccharide-induced septic shock via promoting inflammation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYing He,Yu Peng,Lijian Tao,Zhangzhe Peng,Huixiang YangPubMed 32423810
- Science advances 6:eaba13342020Glutathione dynamics determine the therapeutic efficacy of mesenchymal stem cells for graft-versus-host disease via CREB1-NRF2 pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJisun Lim et. al.PubMed 32490200
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.05% Sodium azide
- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human PRDX1 aa 100-150. The exact immunogen used to generate this antibody is proprietary information. Database link Q06830
Reactivity data
Select an application| WB | IHC-P | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| African green monkey | Tested | Expected | Expected |
| Chinese hamster | Predicted | Predicted | Predicted |
| Cow | Predicted | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 | Notes - |
Species African green monkey | Dilution info 1/1000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Chinese hamster | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species African green monkey | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Chinese hamster | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/200 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species African green monkey | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Chinese hamster | Dilution info - | Notes - |
Associated Products
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Target data
Function
Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively. Plays a role in cell protection against oxidative stress by detoxifying peroxides and as sensor of hydrogen peroxide-mediated signaling events. Might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H(2)O(2) (PubMed:9497357). Reduces an intramolecular disulfide bond in GDPD5 that gates the ability to GDPD5 to drive postmitotic motor neuron differentiation (By similarity).
Alternative names
PAGA, PAGB, TDPX2, PRDX1, Peroxiredoxin-1, Natural killer cell-enhancing factor A, Proliferation-associated gene protein, Thioredoxin peroxidase 2, Thioredoxin-dependent peroxide reductase 2, Thioredoxin-dependent peroxiredoxin 1, NKEF-A, PAG
Recommended products
Knockout Tested Rabbit Polyclonal Peroxiredoxin 1/PAG antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human, African green monkey samples. Cited in 36 publications. Immunogen corresponding to Synthetic Peptide within Human PRDX1 aa 100-150.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.05% Sodium azide
- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human PRDX1 aa 100-150. The exact immunogen used to generate this antibody is proprietary information. Database link Q06830
- Purity
- Whole antiserum
- Specificity
This antibody detects Peroxiredoxin 1 protein in human samples. The antibody is specific for Peroxiredoxin 1/PAG and shows no cross reactivity with other Prx isoforms.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Peroxiredoxin 1 also known as PAG plays an important role as an antioxidant enzyme. It is part of the peroxiredoxin family with a molecular mass of around 22 kDa. This protein helps reduce hydrogen peroxide and organic hydroperoxides to water and corresponding alcohols using reducing equivalents provided by thiol-containing donor molecules. Peroxiredoxin 1/PAG expresses widely in human tissues and cells including liver and erythrocytes reflecting its involvement in cellular redox regulation.
- Biological function summary
Peroxiredoxin 1 helps maintain cellular homeostasis by protecting cells from oxidative stress. It forms homodimers or interacts with other proteins to propagate cellular responses to changes in redox state. As an important member of the antioxidant defense mechanism it contributes to cellular signaling through modulation of hydrogen peroxide signaling pathways. The protein also plays a role in cell proliferation and apoptosis making it significant for cellular health and disease prevention.
- Pathways
Peroxiredoxin 1 participates in critical processes such as the MAPK signaling and TGF-beta pathways. It interacts with MAPK-related proteins influencing cell survival and growth. Peroxiredoxin 1 indirectly modulates downstream signaling molecules that contribute to cellular responses. In these pathways the protein's antioxidant properties allow regulation of reactive oxygen species levels which directly affect cellular signaling cascades.
- Associated diseases and disorders
Peroxiredoxin 1 is relevant to cancer and neurodegenerative disorders. Overexpression or misregulation of this protein has been linked to various cancers where it potentially interacts with oncogenic proteins like c-Myc to promote tumor progression. Additionally abnormal levels of Peroxiredoxin 1 associate with neurodegenerative diseases as oxidative stress is a common factor in their pathology. These connections suggest the protein as a promising target in therapeutic interventions for these diseases.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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6 product images
Western blot - Anti-Peroxiredoxin 1/PAG antibody (ab15571)
Lane 1: Wild-type HAP1 cell lysate (20 μg)
Lane 2: Peroxiredoxin 1/PAG knockout HAP1 cell lysate (20 μg)
Lane 3: A431 cell lysate (20 μg)
Lane 4: Jurkat cell lysate (20 μg)
Lanes 1 - 4: Merged signal (red and green). Green - ab15571 observed at 23 kDa. Red - loading control, Anti-GAPDH antibody [6C5] - Loading Control ab8245, observed at 37 kDa.
ab15571 was shown to recognize Peroxiredoxin 1/PAG when Peroxiredoxin 1/PAG knockout samples were used, along with additional cross-reactive bands. Wild-type and Peroxiredoxin 1/PAG knockout samples were subjected to SDS-PAGE. ab15571 and Anti-GAPDH antibody [6C5] - Loading Control ab8245 (loading control to GAPDH) were diluted to 1/1000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) andGoat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.All lanes: Western blot - Anti-Peroxiredoxin 1/PAG antibody (ab15571)
Predicted band size: 22 kDa
Immunocytochemistry/ Immunofluorescence - Anti-Peroxiredoxin 1/PAG antibody (ab15571)
Immunocytochemistry/ Immunofluorescence analysis of Peroxiredoxin 1/PAG was performed using 70% confluent log phase T-47D cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with ab15571 at 1/250 dilution in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate at a dilution of 1/2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI. F-actin (Panel c: red) was stained with Rhodamine Phalloidin at 1/300. Panel d represents the merged image showing cytosolic and nuclear localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.
Western blot - Anti-Peroxiredoxin 1/PAG antibody (ab15571)
All lanes: Western blot - Anti-Peroxiredoxin 1/PAG antibody (ab15571) at 1/250 dilution
Lane 1: HepG2 whole cell lysate at 30 µg
Lane 2: Raji whole cell lysate at 30 µg
Lane 3: Ramos whole cell lysate at 30 µg
SecondaryAll lanes: Goat anti-Rabbit IgG (H+L), HRP conjugate at 1/4000 dilution
Predicted band size: 22 kDa
Observed band size: 22 kDa
Immunocytochemistry/ Immunofluorescence - Anti-Peroxiredoxin 1/PAG antibody (ab15571)
ICC/IF image of ab15571 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab15571, 1/200 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peroxiredoxin 1/PAG antibody (ab15571)
IHC image of ab15571 staining in human liver carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab15571, 1/100 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Western blot - Anti-Peroxiredoxin 1/PAG antibody (ab15571)
Western blot analysis of Peroxiredoxin 1//PAG was performed by loading 25ug of various whole cell lysates onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% Milk/TBST for at least 1 hour. Membranes were probed with ab15571 overnight at 4°C on a rocking platform. Membranes were washed in TBS-0.1%Tween 20 and probed with a goat anti-rabbit-HRP secondary antibody for at least one hour. Membranes were washed and chemiluminescent detection performed.
All lanes: Western blot - Anti-Peroxiredoxin 1/PAG antibody (ab15571) at 1/1000 dilution
All lanes: Cell line indicated at 25 µg
SecondaryAll lanes: HRP-conjugated Goat anti-rabbit at 1/20000 dilution
Predicted band size: 22 kDa
Observed band size: 20 kDa, 25 kDa
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