Rabbit Polyclonal Peroxiredoxin 1/PAG antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 36 publications.
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- Cancers 14:2022Mutational Activation of the NRF2 Pathway Upregulates Kynureninase Resulting in Tumor Immunosuppression and Poor Outcome in Lung Adenocarcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJohannes F Fahrmann,Ichidai Tanaka,Ehsan Irajizad,Xiangying Mao,Jennifer B Dennison,Eunice Murage,Julian Casabar,Jeffrey Mayo,Qian Peng,Muge Celiktas,Jody V Vykoukal,Soyoung Park,Ayumu Taguchi,Oliver Delgado,Satyendra C Tripathi,Hiroyuki Katayama,Luisa Maren Solis Soto,Jaime Rodriguez-Canales,Carmen Behrens,Ignacio Wistuba,Samir Hanash,Edwin J OstrinPubMed 35626147
- Antioxidants (Basel, Switzerland) 10:2021β-Hydroxybutyrate, a Ketone Body, Potentiates the Antioxidant Defense via Thioredoxin 1 Upregulation in Cardiomyocytes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShin-Ichi Oka,Fan Tang,Adave Chin,Guersom Ralda,Xiaoyong Xu,Chengchen Hu,Zhi Yang,Maha Abdellatif,Junichi SadoshimaPubMed 34356388
- Experimental and therapeutic medicine 20:2692020Dimethyl fumarate protects nucleus pulposus cells from inflammation and oxidative stress and delays the intervertebral disc degeneration.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHainian Zhu,Gang Chen,Yuhua Wang,Xuchen Lin,Jingyuan Zhou,Zengshun Wang,Nanangxiu SuoPubMed 33199994
- Science advances 6:2020CD1d1 intrinsic signaling in macrophages controls NLRP3 inflammasome expression during inflammation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShan Cui et. al.PubMed 33087357
- Free radical biology & medicine 160:566-5742020S-nitrosocysteine and glutathione depletion synergize to induce cell death in human tumor cells: Insights into the redox and cytotoxic mechanisms.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAlaa Knany et. al.PubMed 32898624
- Free radical biology & medicine 160:199-20720202-Cys peroxiredoxin oxidation in response to hydrogen peroxide and contractile activity in skeletal muscle: A novel insight into exercise-induced redox signalling?Applications:Unspecified applicationReactive species:Unspecified reactive speciesClare Stretton et. al.PubMed 32784030
- Redox biology :1016232020Oxidative stress in the retina and retinal pigment epithelium (RPE): Role of aging, and DJ-1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMala Upadhyay et. al.PubMed 32826201
- Antioxidants (Basel, Switzerland) 9:2020The Metabolite Urolithin-A Ameliorates Oxidative Stress in Neuro-2a Cells, Becoming a Potential Neuroprotective Agent.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGuillermo Cásedas et. al.PubMed 32098107
- Redox biology 30:1014172020Peroxiredoxin-1 regulates lipid peroxidation in corneal endothelial cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMatthew Lovatt,Khadijah Adnan,Viridiana Kocaba,Martin Dirisamer,Gary S L Peh,Jodhbir S MehtaPubMed 31901729
- Aging 11:7880-78982019Mechanisms of ROS-induced mitochondria-dependent apoptosis underlying liquid storage of goat spermatozoa.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTengfei Liu et. al.PubMed 31548434
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Consider this alternative
Reactivity data
Select an application| IHC-P | WB | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Predicted | Predicted |
| Rat | Predicted | Predicted |
| Cow | Predicted | Predicted |
| Rabbit | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1 µg/mL | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Rabbit, Cow | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Rabbit, Cow | Dilution info - | Notes - |
Associated Products
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Target data
Function
Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively. Plays a role in cell protection against oxidative stress by detoxifying peroxides and as sensor of hydrogen peroxide-mediated signaling events. Might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H(2)O(2) (PubMed:9497357). Reduces an intramolecular disulfide bond in GDPD5 that gates the ability to GDPD5 to drive postmitotic motor neuron differentiation (By similarity).
Alternative names
PAGA, PAGB, TDPX2, PRDX1, Peroxiredoxin-1, Natural killer cell-enhancing factor A, Proliferation-associated gene protein, Thioredoxin peroxidase 2, Thioredoxin-dependent peroxide reductase 2, Thioredoxin-dependent peroxiredoxin 1, NKEF-A, PAG
Recommended products
Rabbit Polyclonal Peroxiredoxin 1/PAG antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 36 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Peroxiredoxin 1 also known as PAG plays an important role as an antioxidant enzyme. It is part of the peroxiredoxin family with a molecular mass of around 22 kDa. This protein helps reduce hydrogen peroxide and organic hydroperoxides to water and corresponding alcohols using reducing equivalents provided by thiol-containing donor molecules. Peroxiredoxin 1/PAG expresses widely in human tissues and cells including liver and erythrocytes reflecting its involvement in cellular redox regulation.
- Biological function summary
Peroxiredoxin 1 helps maintain cellular homeostasis by protecting cells from oxidative stress. It forms homodimers or interacts with other proteins to propagate cellular responses to changes in redox state. As an important member of the antioxidant defense mechanism it contributes to cellular signaling through modulation of hydrogen peroxide signaling pathways. The protein also plays a role in cell proliferation and apoptosis making it significant for cellular health and disease prevention.
- Pathways
Peroxiredoxin 1 participates in critical processes such as the MAPK signaling and TGF-beta pathways. It interacts with MAPK-related proteins influencing cell survival and growth. Peroxiredoxin 1 indirectly modulates downstream signaling molecules that contribute to cellular responses. In these pathways the protein's antioxidant properties allow regulation of reactive oxygen species levels which directly affect cellular signaling cascades.
- Associated diseases and disorders
Peroxiredoxin 1 is relevant to cancer and neurodegenerative disorders. Overexpression or misregulation of this protein has been linked to various cancers where it potentially interacts with oncogenic proteins like c-Myc to promote tumor progression. Additionally abnormal levels of Peroxiredoxin 1 associate with neurodegenerative diseases as oxidative stress is a common factor in their pathology. These connections suggest the protein as a promising target in therapeutic interventions for these diseases.
Product promise
Tested
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Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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6 product images
Western blot - Anti-Peroxiredoxin 1/PAG antibody (ab41906)
Lane 1: Wild-type HAP1 cell lysate (20 μg)
Lane 2: Peroxiredoxin 1/PAG knockout HAP1 cell lysate (20 μg)
Lane 3: A431 cell lysate (20 μg)
Lane 4: Jurkat cell lysate (20 μg)
Lanes 1 - 4: Merged signal (red and green). Green - ab41906 observed at 23 kDa. Red - loading control, Anti-GAPDH antibody [6C5] - Loading Control ab8245, observed at 37 kDa.
ab41906 was shown to recognize Peroxiredoxin 1/PAG when Peroxiredoxin 1/PAG knockout samples were used, along with additional cross-reactive bands. Wild-type and Peroxiredoxin 1/PAG knockout samples were subjected to SDS-PAGE. ab41906 and Anti-GAPDH antibody [6C5] - Loading Control ab8245 (loading control to GAPDH) at a concentration of 1 μg/ml and diluted to 1/10000 respectively were incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.All lanes: Western blot - Anti-Peroxiredoxin 1/PAG antibody (ab41906)
Predicted band size: 22 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peroxiredoxin 1/PAG antibody (ab41906)
IHC image of Peroxiredoxin 1/PAG staining in human liver carcinoma FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab41906, 1μg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Western blot - Anti-Peroxiredoxin 1/PAG antibody (ab41906)
ab41906 was shown to react with PRDX1 in wild-type U-2 OS cells in Western blot with loss of signal observed in a PRDX1 knockout cell line. Wild-type U-2 OS and PRDX1 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 5% milk in TBST for 1 hr before incubation with ab41906 overnight at 4 °C at a 1/1000 dilution. Blots were incubated with goat anti-rabbit HRP secondary antibodies at 0.2ug/mL before imaging. This data was kindly provided by the YCharOS Inc., an open science company with the mission of characterizing every commercially available antibody reagent. Abcam are working with YCharOS to support their mission of antibody characterisation using knockout cell lines.
All lanes: Western blot - Anti-Peroxiredoxin 1/PAG antibody (ab41906) at 1/1000 dilution
Lane 1: Wild-type U-2 OS cell lysate at 20 µg
Lane 2: PRDX1 knockout U-2 OS cell lysate at 20 µg
Performed under reducing conditions.
Predicted band size: 22 kDa
Western blot - Anti-Peroxiredoxin 1/PAG antibody (ab41906)
All lanes: Western blot - Anti-Peroxiredoxin 1/PAG antibody (ab41906) at 1 µg/mL
Lane 1: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
Lane 2: Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate at 10 µg
Lane 3: A431 (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
Lane 4: HEK293 (Human embryonic kidney cell line) Whole Cell Lysate at 10 µg
Lane 5: HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg
Lane 6: MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate at 10 µg
Lane 7: SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate at 10 µg
Lane 8: U2OS (Human osteosarcoma cell line) Whole Cell Lysate at 10 µg
SecondaryAll lanes: Goat Polyclonal to rabbit IgG - H&L - Pre adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 22 kDa
Observed band size: 24 kDa
Western blot - Anti-Peroxiredoxin 1/PAG antibody (ab41906)
This protein is a homodimer consisting of two subunits with an expected molecular weight of 23kDa.
All lanes: Western blot - Anti-Peroxiredoxin 1/PAG antibody (ab41906) at 1 µg/mL
All lanes: Western blot - Recombinant human Peroxiredoxin 1/PAG protein (Recombinant human Peroxiredoxin 1/PAG protein ab79945) at 0.01 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 22 kDa
Exposure time: 30s
Western blot - Anti-Peroxiredoxin 1/PAG antibody (ab41906)
ab41906 was shown to react with PRDX1 in wild-type U2OS cells in Western blot with loss of signal observed in PRDX1 knockout cell line. Wild-type U2OS and PRDX1 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 5% milk in TBST for 1 hr before incubation with ab41906 overnight at 4 °C at a 1/1000 dilution. Blots were incubated with secondary antibodies at 0.2 µg/mL before imaging.
These data were provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins. Abcam and YCharOS are working together to help address the reproducibility crisis by enabling the life science community to better evaluate commercially available antibodies.All lanes: Western blot - Anti-Peroxiredoxin 1/PAG antibody (ab41906) at 1/1000 dilution
Lane 1: Wild-type U2OS lysate at 10 µg
Lane 2: PRDX1 knock-out U2OS lysate at 10 µg
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