Anti-Peroxiredoxin 1/PAG antibody

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peroxiredoxin 1/PAG antibody (AB41906)

IHC image of Peroxiredoxin 1/PAG staining in human liver carcinoma FFPE section, performed on a Bond^TM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab41906, 1μg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

• WB

Collaborator

Western blot - Anti-Peroxiredoxin 1/PAG antibody (AB41906)

ab41906 was shown to react with PRDX1 in wild-type U-2 OS cells in Western blot with loss of signal observed in a PRDX1 knockout cell line. Wild-type U-2 OS and PRDX1 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 5% milk in TBST for 1 hr before incubation with ab41906 overnight at 4 °C at a 1/1000 dilution. Blots were incubated with goat anti-rabbit HRP secondary antibodies at 0.2ug/mL before imaging. This data was kindly provided by the YCharOS Inc., an open science company with the mission of characterizing every commercially available antibody reagent. Abcam are working with YCharOS to support their mission of antibody characterisation using knockout cell lines.

All lanes:

Western blot - Anti-Peroxiredoxin 1/PAG antibody (ab41906) at 1/1000 dilution

Lane 1:

Wild-type U-2 OS cell lysate at 20 µg

Lane 2:

PRDX1 knockout U-2 OS cell lysate at 20 µg

Predicted band size: 22 kDa

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• WB

Lab

Western blot - Anti-Peroxiredoxin 1/PAG antibody (AB41906)

Lane 1 : Wild-type HAP1 cell lysate (20 μg)
Lane 2 : Peroxiredoxin 1/PAG knockout HAP1 cell lysate (20 μg)
Lane 3 : A431 cell lysate (20 μg)
Lane 4 : Jurkat cell lysate (20 μg)
Lanes 1 - 4 : Merged signal (red and green). Green - ab41906 observed at 23 kDa. Red - loading control, ab8245, observed at 37 kDa.

ab41906 was shown to recognize Peroxiredoxin 1/PAG when Peroxiredoxin 1/PAG knockout samples were used, along with additional cross-reactive bands. Wild-type and Peroxiredoxin 1/PAG knockout samples were subjected to SDS-PAGE. ab41906 and ab8245 (loading control to GAPDH) at a concentration of 1 μg/ml and diluted to 1/10000 respectively were incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Peroxiredoxin 1/PAG antibody (ab41906)

Predicted band size: 22 kDa

false

• WB

Project3009****

Western blot - Anti-Peroxiredoxin 1/PAG antibody (AB41906)

All lanes:

Western blot - Anti-Peroxiredoxin 1/PAG antibody (ab41906) at 1 µg/mL

Lane 1:

HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

Lane 2:

Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate at 10 µg

Lane 3:

A431 (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

Lane 4:

HEK293 (Human embryonic kidney cell line) Whole Cell Lysate at 10 µg

Lane 5:

HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg

Lane 6:

MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate at 10 µg

Lane 7:

SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate at 10 µg

Lane 8:

U2OS (Human osteosarcoma cell line) Whole Cell Lysate at 10 µg

Secondary

All lanes:

Goat Polyclonal to rabbit IgG - H&L - Pre adsorbed (HRP) at 1/3000 dilution

Predicted band size: 22 kDa

Observed band size: 24 kDa

false

• WB

Lab

Western blot - Anti-Peroxiredoxin 1/PAG antibody (AB41906)

ab41906 was shown to react with PRDX1 in wild-type U2OS cells in Western blot with loss of signal observed in PRDX1 knockout cell line. Wild-type U2OS and PRDX1 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 5% milk in TBST for 1 hr before incubation with ab41906 overnight at 4 °C at a 1/1000 dilution. Blots were incubated with secondary antibodies at 0.2 µg/mL before imaging.

These data were provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins. Abcam and YCharOS are working together to help address the reproducibility crisis by enabling the life science community to better evaluate commercially available antibodies.

All lanes:

Western blot - Anti-Peroxiredoxin 1/PAG antibody (ab41906) at 1/1000 dilution

Lane 1:

Wild-type U2OS lysate at 10 µg

Lane 2:

PRDX1 knock-out U2OS lysate at 10 µg

false

• WB

Unknown

Western blot - Anti-Peroxiredoxin 1/PAG antibody (AB41906)

This protein is a homodimer consisting of two subunits with an expected molecular weight of 23kDa.

All lanes:

Western blot - Anti-Peroxiredoxin 1/PAG antibody (ab41906) at 1 µg/mL

All lanes:

Western blot - Recombinant human Peroxiredoxin 1/PAG protein (<a href='/en-us/products/proteins-peptides/recombinant-human-peroxiredoxin-1-pag-protein-ab79945'>ab79945</a>) at 0.01 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

Predicted band size: 22 kDa

true

Exposure time: 30s