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Rabbit Recombinant Monoclonal Peroxiredoxin 1/PAG antibody. Suitable for IHC-P, WB and reacts with Mouse, Human samples. Cited in 7 publications.

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Images

Western blot - Anti-Peroxiredoxin 1/PAG antibody [EPR5433] (AB109498), expandable thumbnail
  • Western blot - Anti-Peroxiredoxin 1/PAG antibody [EPR5433] (AB109498), expandable thumbnail
  • Western blot - Anti-Peroxiredoxin 1/PAG antibody [EPR5433] (AB109498), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peroxiredoxin 1/PAG antibody [EPR5433] (AB109498), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peroxiredoxin 1/PAG antibody [EPR5433] (AB109498), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PIPWB
Human
Tested
Not recommended
Tested
Mouse
Tested
Not recommended
Tested

Tested
Tested

Species
Mouse
Dilution info
1/1000 - 1/4000
Notes

Perform antigen retrieval

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Species
Human
Dilution info
1/1000 - 1/4000
Notes

Perform antigen retrieval

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Mouse, Human
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/10000 - 1/50000
Notes

-

Species
Human
Dilution info
1/10000 - 1/50000
Notes

-

Associated Products

Select an associated product type

5 products for Alternative Product

Target data

Function

Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively. Plays a role in cell protection against oxidative stress by detoxifying peroxides and as sensor of hydrogen peroxide-mediated signaling events. Might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H(2)O(2) (PubMed:9497357). Reduces an intramolecular disulfide bond in GDPD5 that gates the ability to GDPD5 to drive postmitotic motor neuron differentiation (By similarity).

Alternative names

PAGA, PAGB, TDPX2, PRDX1, Peroxiredoxin-1, Natural killer cell-enhancing factor A, Proliferation-associated gene protein, Thioredoxin peroxidase 2, Thioredoxin-dependent peroxide reductase 2, Thioredoxin-dependent peroxiredoxin 1, NKEF-A, PAG

Recommended products

Rabbit Recombinant Monoclonal Peroxiredoxin 1/PAG antibody. Suitable for IHC-P, WB and reacts with Mouse, Human samples. Cited in 7 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR5433
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Peroxiredoxin 1 also known as PAG plays an important role as an antioxidant enzyme. It is part of the peroxiredoxin family with a molecular mass of around 22 kDa. This protein helps reduce hydrogen peroxide and organic hydroperoxides to water and corresponding alcohols using reducing equivalents provided by thiol-containing donor molecules. Peroxiredoxin 1/PAG expresses widely in human tissues and cells including liver and erythrocytes reflecting its involvement in cellular redox regulation.

Biological function summary

Peroxiredoxin 1 helps maintain cellular homeostasis by protecting cells from oxidative stress. It forms homodimers or interacts with other proteins to propagate cellular responses to changes in redox state. As an important member of the antioxidant defense mechanism it contributes to cellular signaling through modulation of hydrogen peroxide signaling pathways. The protein also plays a role in cell proliferation and apoptosis making it significant for cellular health and disease prevention.

Pathways

Peroxiredoxin 1 participates in critical processes such as the MAPK signaling and TGF-beta pathways. It interacts with MAPK-related proteins influencing cell survival and growth. Peroxiredoxin 1 indirectly modulates downstream signaling molecules that contribute to cellular responses. In these pathways the protein's antioxidant properties allow regulation of reactive oxygen species levels which directly affect cellular signaling cascades.

Associated diseases and disorders

Peroxiredoxin 1 is relevant to cancer and neurodegenerative disorders. Overexpression or misregulation of this protein has been linked to various cancers where it potentially interacts with oncogenic proteins like c-Myc to promote tumor progression. Additionally abnormal levels of Peroxiredoxin 1 associate with neurodegenerative diseases as oxidative stress is a common factor in their pathology. These connections suggest the protein as a promising target in therapeutic interventions for these diseases.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Western blot - Anti-Peroxiredoxin 1/PAG antibody [EPR5433] (ab109498), expandable thumbnail

    Western blot - Anti-Peroxiredoxin 1/PAG antibody [EPR5433] (ab109498)

    Lane 1: Wild-type HAP1 cell lysate (20 μg)
    Lane 2: Peroxiredoxin 1/PAG knockout HAP1 cell lysate (20 μg)
    Lane 3: A431 cell lysate (20 μg)
    Lane 4: Jurkat cell lysate (20 μg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab109498 observed at 23 kDa. Red - loading control, Anti-GAPDH antibody [6C5] - Loading Control ab8245, observed at 37 kDa.

    ab109498 was shown to specifically react with Peroxiredoxin 1/PAG when Peroxiredoxin 1/PAG knockout samples were used. Wild-type and Peroxiredoxin 1/PAG knockout samples were subjected to SDS-PAGE. ab109498 and Anti-GAPDH antibody [6C5] - Loading Control ab8245 (loading control to GAPDH) were both diluted 1/10 000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

    All lanes: Western blot - Anti-Peroxiredoxin 1/PAG antibody [EPR5433] (ab109498)

    Predicted band size: 22 kDa

  • Western blot - Anti-Peroxiredoxin 1/PAG antibody [EPR5433] (ab109498), expandable thumbnail

    Western blot - Anti-Peroxiredoxin 1/PAG antibody [EPR5433] (ab109498)

    Lanes 1-3: Merged signal (red and green). Green - ab109498 observed at 26 kDa. Red - loading control Anti-GAPDH antibody [6C5] - Loading Control ab8245 observed at 36 kDa.

    ab109498 Anti-Peroxiredoxin 1/PAG antibody [EPR5433] was shown to specifically react with Peroxiredoxin 1/PAG in wild-type HEK293T cells. Loss of signal was observed when knockout cell line Human PRDX1 (Peroxiredoxin 1/PAG) knockout HEK-293T cell line ab266842 (knockout cell lysate Human PRDX1 (Peroxiredoxin 1/PAG) knockout HEK-293T cell lysate ab257040) was used. Wild-type and Peroxiredoxin 1/PAG knockout samples were subjected to SDS-PAGE. ab109498 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-Peroxiredoxin 1/PAG antibody [EPR5433] (ab109498) at 1/1000 dilution

    Lane 1: Wild-type HEK293T cell lysate at 20 µg

    Lane 2: PRDX1 knockout HEK293T cell lysate at 20 µg

    Lane 2: Western blot - Human PRDX1 (Peroxiredoxin 1/PAG) knockout HEK-293T cell line (Human PRDX1 (Peroxiredoxin 1/PAG) knockout HEK-293T cell line ab266842)

    Lane 3: Jurkat cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) at 1/10000 dilution

    Predicted band size: 22 kDa, 41 kDa, 45 kDa, 78 kDa

    Observed band size: 25 kDa, 26 kDa, 47 kDa, 49 kDa, 78 kDa

  • Western blot - Anti-Peroxiredoxin 1/PAG antibody [EPR5433] (ab109498), expandable thumbnail

    Western blot - Anti-Peroxiredoxin 1/PAG antibody [EPR5433] (ab109498)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    All lanes: Western blot - Anti-Peroxiredoxin 1/PAG antibody [EPR5433] (ab109498) at 1/10000 dilution

    Lane 1: MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 2: K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysate at 20 µg

    Lane 3: NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate at 20 µg

    Lane 4: Mouse brain lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 22 kDa

    Observed band size: 22 kDa

    Exposure time: 10s

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peroxiredoxin 1/PAG antibody [EPR5433] (ab109498), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peroxiredoxin 1/PAG antibody [EPR5433] (ab109498)

    Immunohistochemical analysis of paraffin-embedded Human thyroid cancer tissue labeling Peroxiredoxin 1/PAG with ab109498 at 1/1000 (0.155 ug/ml) dilution. The section was incubated with ab109498 for 30 mins at room temperature and followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Counterstained with Hematoxylin.

    Positive staining on the human thyroid cancer, performed on a Leica Biosystems BOND® RX instrument.

    Secondary antibody only control: Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101)

    Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peroxiredoxin 1/PAG antibody [EPR5433] (ab109498), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peroxiredoxin 1/PAG antibody [EPR5433] (ab109498)

    Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling Peroxiredoxin 1/PAG with ab109498 at 1/4000 (0.038 ug/ml) dilution. The section was incubated with ab109498 for 30 mins at room temperature and followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Counterstained with Hematoxylin.

    Positive staining on the mouse kidney, performed on a Leica Biosystems BOND® RX instrument.

    Secondary antibody only control: Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101)

    Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

  • Western blot - Anti-Peroxiredoxin 1/PAG antibody [EPR5433] (ab109498), expandable thumbnail

    Image collected and cropped by CiteAb under a CC-BY license from the publication

    Western blot - Anti-Peroxiredoxin 1/PAG antibody [EPR5433] (ab109498)

    Peroxiredoxin 1/PAG western blot using anti-Peroxiredoxin 1/PAG antibody [EPR5433] ab109498. Publication image and figure legend from Ziegler, Y. S., Moresco, J. J., et al., 2016, PLoS One, PubMed 27355404.


    ab109498 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab109498 please see the product overview.

    Identification of proteins in BC and MCF-10A cell secretomes.A. A workflow was established to analyze the secretomes of BC and benign mammary epithelial cells. B. Benign (MCF-10A), ERα-positive BC (MCF-7), and TNBC (MDA-MB-231, DT22, and DT28) cells were established in 3D cultures using Matrigel extracellular matrix. Live (top row) and fixed (bottom row) cultures were imaged by phase contrast or immunofluorescence, respectively. Markers of proliferation (Ki67-red), basement membrane (laminin-green), and nuclei (DAPI-blue) were utilized. C. 3D cultures of MCF-10A cells were exposed to CM from MCF-10A, MCF-7, MDA-MB-231, DT22, or DT28 cells. Proliferation (red) and nuclei (blue) are indicated by Ki67 and DAPI staining, respectively. Scale bars indicate 25 μm. D. 3D cultures described in panel C were analyzed to determine the percent of proliferating MCF-10A cells for each treatment. An asterisk (*) indicates a p-value <0.05. E. CM from each cell line was subjected to MS and compiled results are shown. F. CM from 3D cultures of BC and MCF-10A cells were subjected to Western blot analysis using antibodies to cathepsin D (CTSD), extracellular matrix protein 1 (ECM1), peroxiredoxin 1 (PRDX1), or 14-3-3 sigma (SFN). The number of spectral counts (SCs) is indicated above each lane. To visualize SFN, the CM was concentrated before blot. MDA-MB-231 and MCF-10A cells are labeled as 231 and 10A, respectively. (Abbreviations: BC = breast cancer, TNBC = triple negative breast cancer, 3D = three dimensional, CM = conditioned medium, MS = mass spectroscopy)

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