Anti-Peroxiredoxin 1/PAG antibody [EPR5434] - BSA and Azide free (ab211538)

Rabbit Recombinant Monoclonal Peroxiredoxin 1/PAG antibody. Carrier free. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

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Images

Western blot - Anti-Peroxiredoxin 1/PAG antibody [EPR5434] - BSA and Azide free (AB211538), expandable thumbnail

Publications

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Constituents: PBS
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	IP	WB	IHC-P	ICC/IF	Flow Cyt (Intra)
Human	Tested	Tested	Not recommended	Tested	Tested
Mouse	Predicted	Predicted	Not recommended	Predicted	Predicted
Rat	Predicted	Predicted	Not recommended	Predicted	Predicted

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes -

Predicted
Predicted

Species	Dilution info	Notes
Species Rat, Mouse	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes -

Predicted
Predicted

Species	Dilution info	Notes
Species Rat, Mouse	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human	Dilution info -	Notes For antigen retrieval, heat up to 98 degree C, below boiling, and then let cool for 10-20 minutes. Perform heat-mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
Species Rat	Dilution info -	Notes -
Species Mouse	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes -

Predicted
Predicted

Species	Dilution info	Notes
Species Rat, Mouse	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes -

Predicted
Predicted

Species	Dilution info	Notes
Species Rat, Mouse	Dilution info -	Notes -

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Target data

See full target information PRDX1

Function

Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively. Plays a role in cell protection against oxidative stress by detoxifying peroxides and as sensor of hydrogen peroxide-mediated signaling events. Might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H(2)O(2) (PubMed:9497357). Reduces an intramolecular disulfide bond in GDPD5 that gates the ability to GDPD5 to drive postmitotic motor neuron differentiation (By similarity).

Alternative names

PAGA, PAGB, TDPX2, PRDX1, Peroxiredoxin-1, Natural killer cell-enhancing factor A, Proliferation-associated gene protein, Thioredoxin peroxidase 2, Thioredoxin-dependent peroxide reductase 2, Thioredoxin-dependent peroxiredoxin 1, NKEF-A, PAG

Recommended products

Rabbit Recombinant Monoclonal Peroxiredoxin 1/PAG antibody. Carrier free. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Constituents: PBS
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Carrier free: Yes
Clone number: EPR5434
Purification technique: Affinity purification Protein A
Specificity: Corresponding to residues in Human Peroxiredoxin 1/PAG.
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: +4°C
Storage information: Do Not Freeze

Notes

ab211538 is the carrier-free version of Anti-Peroxiredoxin 1/PAG antibody [EPR5434] ab109506.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Peroxiredoxin 1 also known as PAG plays an important role as an antioxidant enzyme. It is part of the peroxiredoxin family with a molecular mass of around 22 kDa. This protein helps reduce hydrogen peroxide and organic hydroperoxides to water and corresponding alcohols using reducing equivalents provided by thiol-containing donor molecules. Peroxiredoxin 1/PAG expresses widely in human tissues and cells including liver and erythrocytes reflecting its involvement in cellular redox regulation.

Biological function summary

Peroxiredoxin 1 helps maintain cellular homeostasis by protecting cells from oxidative stress. It forms homodimers or interacts with other proteins to propagate cellular responses to changes in redox state. As an important member of the antioxidant defense mechanism it contributes to cellular signaling through modulation of hydrogen peroxide signaling pathways. The protein also plays a role in cell proliferation and apoptosis making it significant for cellular health and disease prevention.

Pathways

Peroxiredoxin 1 participates in critical processes such as the MAPK signaling and TGF-beta pathways. It interacts with MAPK-related proteins influencing cell survival and growth. Peroxiredoxin 1 indirectly modulates downstream signaling molecules that contribute to cellular responses. In these pathways the protein's antioxidant properties allow regulation of reactive oxygen species levels which directly affect cellular signaling cascades.

Associated diseases and disorders

Peroxiredoxin 1 is relevant to cancer and neurodegenerative disorders. Overexpression or misregulation of this protein has been linked to various cancers where it potentially interacts with oncogenic proteins like c-Myc to promote tumor progression. Additionally abnormal levels of Peroxiredoxin 1 associate with neurodegenerative diseases as oxidative stress is a common factor in their pathology. These connections suggest the protein as a promising target in therapeutic interventions for these diseases.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

9 product images

Western blot - Anti-Peroxiredoxin 1/PAG antibody [EPR5434] - BSA and Azide free (ab211538)
This data was developed using the same antibody clone in a different buffer formulation (Anti-Peroxiredoxin 1/PAG antibody [EPR5434] ab109506).
Lanes 1-3: Merged signal (red and green). Green - Anti-Peroxiredoxin 1/PAG antibody [EPR5434] ab109506 observed at 26 kDa. Red - loading control Anti-GAPDH antibody [6C5] - Loading Control ab8245 observed at 36 kDa.
Anti-Peroxiredoxin 1/PAG antibody [EPR5434] ab109506 Anti-Peroxiredoxin 1/PAG antibody [EPR5434] was shown to specifically react with Peroxiredoxin in wild-type HEK293T cells. Loss of signal was observed when knockout cell line Human PRDX1 (Peroxiredoxin 1/PAG) knockout HEK-293T cell line ab266842 (knockout cell lysate Human PRDX1 (Peroxiredoxin 1/PAG) knockout HEK-293T cell lysate ab257040) was used. Wild-type and Peroxiredoxin knockout samples were subjected to SDS-PAGE. Anti-Peroxiredoxin 1/PAG antibody [EPR5434] ab109506 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-Peroxiredoxin 1/PAG antibody [EPR5434] (Anti-Peroxiredoxin 1/PAG antibody [EPR5434] ab109506) at 1/1000 dilution
Lane 1: Wild-type HEK293T cell lysate at 20 µg
Lane 2: PRDX1 knockout HEK293T cell lysate at 20 µg
Lane 2: Western blot - Human PRDX1 (Peroxiredoxin 1/PAG) knockout HEK-293T cell line (Human PRDX1 (Peroxiredoxin 1/PAG) knockout HEK-293T cell line ab266842)
Lane 3: Jurkat cell lysate at 20 µg
Secondary
All lanes: Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) at 1/10000 dilution
Predicted band size: 22 kDa
Observed band size: 26 kDa
Immunoprecipitation - Anti-Peroxiredoxin 1/PAG antibody [EPR5434] - BSA and Azide free (ab211538)
This data was developed using the same antibody in a different buffer formulation (Anti-Peroxiredoxin 1/PAG antibody [EPR5434] ab109506).
Immunoprecipitation of PRDX1 in U-2 OS cells. Lysates were prepared and immunoprecipitation was performed using 1.0 μg of Anti-Peroxiredoxin 1/PAG antibody [EPR5434] ab109506 pre-coupled to prot.A-Sepharose beads. Samples were washed and processed for western blot with Peroxiredoxin 1 Antibody at 1/10000. SM=10% starting material; UB=10% unbound fraction; IP=immunoprecipitate. These data were provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins. Abcam and YCharOS are working together to help address the reproducibility crisis by enabling the life science community to better evaluate commercially available antibodies.
All lanes: Immunoprecipitation - Anti-Peroxiredoxin 1/PAG antibody [EPR5434] - BSA and Azide free (ab211538)
Predicted band size: 22 kDa
Immunocytochemistry/ Immunofluorescence - Anti-Peroxiredoxin 1/PAG antibody [EPR5434] - BSA and Azide free (ab211538)
This data was developed using the same antibody clone in a different buffer formulation (Anti-Peroxiredoxin 1/PAG antibody [EPR5434] ab109506). Anti-Peroxiredoxin 1/PAG antibody [EPR5434] ab109506 was shown to react with PRDX1 in wild-type U-2 OS cells in Immunocytochemistry with loss of signal observed in a PRDX1 knockout cell line. Wild-type and knockout cells were mixed and pelleted at a 1:1 ratio on coverslips. The cells were fixed with then permeabilized with and then blocked with 1/10000. The cells were then incubated with Anti-Peroxiredoxin 1/PAG antibody [EPR5434] ab109506 at 1/250. 1/5000 would be better dilution overnight at 4°C followed by a further incubation at room temperature for 1h with a secondary antibody to (Alexa Fluor^® 555) at 0.5 μg/ml. Acquisition of the green (wild-type), red (antibody staining) and far-red (knockout) channels was performed. Representative grayscale images of the red channel are shown. Wild-type and knockout cells are outlined with yellow and magenta dashed line, respectively. Schematic representation of the mosaic strategy used is shown on the bottom-right panel. Image was acquired with a Zeiss(LSM-880). These data were provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins. Abcam and YCharOS are working together to help address the reproducibility crisis by enabling the life science community to better evaluate commercially available antibodies.
Immunocytochemistry/ Immunofluorescence - Anti-Peroxiredoxin 1/PAG antibody [EPR5434] - BSA and Azide free (ab211538)
This data was developed using the same antibody clone in a different buffer formulation (Anti-Peroxiredoxin 1/PAG antibody [EPR5434] ab109506).
Peroxiredoxin 1/PAG (PRDX1) staining observed in wild-type HEK293T cells and PRDX1 knockout HEK293T cells (Human PRDX1 (Peroxiredoxin 1/PAG) knockout HEK-293T cell line ab266842). The cells were fixed with 100% methanol then permeabilized with 0.1% Triton X-100. The cells were then incubated with Anti-Peroxiredoxin 1/PAG antibody [EPR5434] ab109506 at 1/50 dilution and followed by secondary antibody Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution (shown in green). Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counterstain at 1/200 dilution (shown in red).Nuclear DNA was labelled in blue with DAPI.
Confocal image showing cytoplasmic staining in wild-type HEK-293Tcell line, and no staining in PRDX1 knockout HEK-293T cell line.
Immunocytochemistry/ Immunofluorescence - Anti-Peroxiredoxin 1/PAG antibody [EPR5434] - BSA and Azide free (ab211538)
This data was developed using the same antibody in a different buffer formulation (Anti-Peroxiredoxin 1/PAG antibody [EPR5434] ab109506).
ICC/IF image of Anti-Peroxiredoxin 1/PAG antibody [EPR5434] ab109506 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (Anti-Peroxiredoxin 1/PAG antibody [EPR5434] ab109506, 1/100 dilution) overnight at +4°C. The secondary antibody (green) was Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.
Western blot - Anti-Peroxiredoxin 1/PAG antibody [EPR5434] - BSA and Azide free (ab211538)
This data was developed using the same antibody in a different buffer formulation (Anti-Peroxiredoxin 1/PAG antibody [EPR5434] ab109506).
Anti-Peroxiredoxin 1/PAG antibody [EPR5434] ab109506 was shown to react with PRDX1 in wild-type U-2 OS cells in Western blot with loss of signal observed in a PRDX1 knockout cell line. Wild-type U-2 OS and PRDX1 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 5% milk in TBST for 1 hr before incubation with Anti-Peroxiredoxin 1/PAG antibody [EPR5434] ab109506 overnight at 4 °C at a 1/5000 dilution. Blots were incubated with goat anti-rabbit HRP secondary antibodies at 0.2ug/mL before imaging. These data were provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins. Abcam and YCharOS are working together to help address the reproducibility crisis by enabling the life science community to better evaluate commercially available antibodies.
All lanes: Western blot - Anti-Peroxiredoxin 1/PAG antibody [EPR5434] (Anti-Peroxiredoxin 1/PAG antibody [EPR5434] ab109506) at 1/5000 dilution
Lane 1: Wild-type U-2 OS cell lysate at 20 µg
Lane 2: PRDX1 knockout U-2 OS cell lysate at 20 µg
Performed under reducing conditions.
Predicted band size: 22 kDa
Flow Cytometry (Intracellular) - Anti-Peroxiredoxin 1/PAG antibody [EPR5434] - BSA and Azide free (ab211538)
This data was developed using the same antibody in a different buffer formulation (Anti-Peroxiredoxin 1/PAG antibody [EPR5434] ab109506).
Intracellular Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling Peroxiredoxin 1/PAG with unpurified Anti-Peroxiredoxin 1/PAG antibody [EPR5434] ab109506 at 1/20 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor^®488) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.
Western blot - Anti-Peroxiredoxin 1/PAG antibody [EPR5434] - BSA and Azide free (ab211538)
This data was developed using the same antibody in a different buffer formulation (Anti-Peroxiredoxin 1/PAG antibody [EPR5434] ab109506).
Lane 1: Wild-type HAP1 cell lysate (20 μg)
Lane 2: Peroxiredoxin 1/PAG knockout HAP1 cell lysate (20 μg)
Lane 3: A431 cell lysate (20 μg)
Lane 4: Jurkat cell lysate (20 μg)
Lanes 1 - 4: Merged signal (red and green). Green - Anti-Peroxiredoxin 1/PAG antibody [EPR5434] ab109506 observed at 23 kDa. Red - loading control, Anti-GAPDH antibody [6C5] - Loading Control ab8245, observed at 37 kDa.
Anti-Peroxiredoxin 1/PAG antibody [EPR5434] ab109506 was shown to specifically react with Peroxiredoxin 1/PAG when Peroxiredoxin 1/PAG knockout samples were used. Wild-type and Peroxiredoxin 1/PAG knockout samples were subjected to SDS-PAGE. Anti-Peroxiredoxin 1/PAG antibody [EPR5434] ab109506 and Anti-GAPDH antibody [6C5] - Loading Control ab8245 (loading control to GAPDH) were both diluted 1/10 000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.
All lanes: Western blot - Anti-Peroxiredoxin 1/PAG antibody [EPR5434] - BSA and Azide free (ab211538)
Predicted band size: 22 kDa
Western blot - Anti-Peroxiredoxin 1/PAG antibody [EPR5434] - BSA and Azide free (ab211538)
All lanes: Western blot - Anti-Peroxiredoxin 1/PAG antibody [EPR5434] - BSA and Azide free (ab211538) at 1/10000 dilution
Lane 1: 293T cell lysate at 10 µg
Lane 2: K562 cell lysate at 10 µg
Lane 3: U87-MG cell lysate at 10 µg
Secondary
All lanes: Standard HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 22 kDa

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Anti-Peroxiredoxin 1/PAG antibody [EPR5434] - BSA and Azide free

Images

Publications

Key facts

Immunogen

Reactivity data

IP

TestedTested

PredictedPredicted

WB

TestedTested

PredictedPredicted

IHC-P

Not recommendedNot recommended

ICC/IF

TestedTested

PredictedPredicted

Flow Cyt (Intra)

TestedTested

PredictedPredicted

Associated Products

1 product for Alternative Product

Target data

Function

Alternative names

Recommended products

Key facts

Storage

Notes

Supplementary info

Product promise

Tested

Expected

Predicted

Not recommended

9 product images

Western blot - Anti-Peroxiredoxin 1/PAG antibody [EPR5434] - BSA and Azide free (ab211538)

Immunoprecipitation - Anti-Peroxiredoxin 1/PAG antibody [EPR5434] - BSA and Azide free (ab211538)

Immunocytochemistry/ Immunofluorescence - Anti-Peroxiredoxin 1/PAG antibody [EPR5434] - BSA and Azide free (ab211538)

Immunocytochemistry/ Immunofluorescence - Anti-Peroxiredoxin 1/PAG antibody [EPR5434] - BSA and Azide free (ab211538)

Immunocytochemistry/ Immunofluorescence - Anti-Peroxiredoxin 1/PAG antibody [EPR5434] - BSA and Azide free (ab211538)

Western blot - Anti-Peroxiredoxin 1/PAG antibody [EPR5434] - BSA and Azide free (ab211538)

Flow Cytometry (Intracellular) - Anti-Peroxiredoxin 1/PAG antibody [EPR5434] - BSA and Azide free (ab211538)

Western blot - Anti-Peroxiredoxin 1/PAG antibody [EPR5434] - BSA and Azide free (ab211538)

Western blot - Anti-Peroxiredoxin 1/PAG antibody [EPR5434] - BSA and Azide free (ab211538)

Downloads

Product protocols

Tested
Tested

Predicted
Predicted

Tested
Tested

Predicted
Predicted

Not recommended
Not recommended

Tested
Tested

Predicted
Predicted

Tested
Tested

Predicted
Predicted