Anti-Peroxiredoxin 2/PRP antibody [EPR5154] - BSA and Azide free (ab227988)

Rabbit Recombinant Monoclonal Peroxiredoxin 2/PRP antibody. Carrier free. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 5 publications.

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Images

Western blot - Anti-Peroxiredoxin 2/PRP antibody [EPR5154] - BSA and Azide free (AB227988), expandable thumbnail

Publications

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Constituents: PBS
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	IHC-P	WB	ICC/IF	Flow Cyt (Intra)
Human	Tested	Tested	Tested	Tested
Mouse	Expected	Tested	Expected	Expected
Rat	Expected	Tested	Expected	Expected
Pig	Predicted	Predicted	Predicted	Predicted

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Predicted
Predicted

Species	Dilution info	Notes
Species Pig	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human, Mouse, Rat	Dilution info -	Notes -

Predicted
Predicted

Species	Dilution info	Notes
Species Pig	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Predicted
Predicted

Species	Dilution info	Notes
Species Pig	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes ab199376 - Rabbit monoclonal IgG (Low endotoxin, Azide free), is suitable for use as an isotype control with this antibody.

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Predicted
Predicted

Species	Dilution info	Notes
Species Pig	Dilution info -	Notes -

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Target data

See full target information PRDX2

Function

Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively. Plays a role in cell protection against oxidative stress by detoxifying peroxides and as sensor of hydrogen peroxide-mediated signaling events. Might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H(2)O(2).

Alternative names

NKEFB, TDPX1, PRDX2, Peroxiredoxin-2, Natural killer cell-enhancing factor B, PRP, Thiol-specific antioxidant protein, Thioredoxin peroxidase 1, Thioredoxin-dependent peroxide reductase 1, Thioredoxin-dependent peroxiredoxin 2, NKEF-B, TSA

Recommended products

Rabbit Recombinant Monoclonal Peroxiredoxin 2/PRP antibody. Carrier free. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 5 publications.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Constituents: PBS
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Carrier free: Yes
Clone number: EPR5154
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: +4°C
Storage information: Do Not Freeze

Notes

ab227988 is the carrier-free version of Anti-Peroxiredoxin 2/PRP antibody [EPR5154] ab109367.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Peroxiredoxin 2 (also known as PRP or PRDX2) is an antioxidant enzyme that plays an important role in reducing peroxides and protecting cells from oxidative damage. The target consisting of approximately 22kDa mass is expressed heavily in erythrocytes but can also be found in other tissues like the heart and liver. PRDX2 belongs to the peroxiredoxin family and its redox activity contributes significantly to cellular homeostasis and defense against oxidative stress.

Biological function summary

Peroxiredoxins like PRDX2 function by breaking down hydrogen peroxide and organic hydroperoxides safeguarding cells from oxidative harm. PRDX2 often forms homodimers or higher-order oligomers which influence its catalytic efficiency and chaperone activity. Within the cell it not only acts independently but also associates with other cellular components reflecting its participation in important cellular processes including cell proliferation and apoptosis.

Pathways

PRDX2 engages significantly within antioxidant defense systems specifically the thioredoxin pathway. It interacts with thioredoxin reductase and thioredoxin to facilitate the reduction of peroxides maintaining the cell’s redox balance. Furthermore PRDX2 intersects with cellular signaling pathways associated with inflammation and the regulation of cell death where proteins like NRF2 and KEAP1 play important roles in managing oxidant-inducible gene expression.

Associated diseases and disorders

Disruptions in PRDX2 function connect to conditions like cancer and cardiovascular diseases. In cancer altered peroxiredoxin 2 expression correlates to tumor progression and resistance to chemotherapy implicating its interaction with proteins like NF-κB in enhancing cell survival. In cardiovascular diseases oxidative stress mediated by PRDX2 imbalance can lead to myocardial infarction and heart failure linking it to proteins such as SOD2 involved in mitochondrial defense.

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10 product images

Western blot - Anti-Peroxiredoxin 2/PRP antibody [EPR5154] - BSA and Azide free (ab227988)
This WB data was generated using the same anti-Peroxiredoxin 2/PRP antibody clone, EPR5154, in a different buffer formulation (cat# Anti-Peroxiredoxin 2/PRP antibody [EPR5154] ab109367).
Lane 1: Wild-type HAP1 cell lysate (20 μg)
Lane 2: Peroxiredoxin 2/PRP knockout HAP1 cell lysate (20 μg)
Lane 3: HeLa cell lysate (20 μg)
Lane 4: LnCaP cell lysate (20 μg)
Lanes 1 - 4: Merged signal (red and green). Green - Anti-Peroxiredoxin 2/PRP antibody [EPR5154] ab109367 observed at 24 kDa. Red - loading control, Anti-GAPDH antibody [6C5] - Loading Control ab8245, observed at 37 kDa.
Anti-Peroxiredoxin 2/PRP antibody [EPR5154] ab109367 was shown to specifically react with Peroxiredoxin 2/PRP when Peroxiredoxin 2/PRP knockout samples were used. Wild-type and Peroxiredoxin 2/PRP knockout samples were subjected to SDS-PAGE. Anti-Peroxiredoxin 2/PRP antibody [EPR5154] ab109367 and Anti-GAPDH antibody [6C5] - Loading Control ab8245 (loading control to GAPDH) were both diluted 1/1000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.
All lanes: Western blot - Anti-Peroxiredoxin 2/PRP antibody [EPR5154] (Anti-Peroxiredoxin 2/PRP antibody [EPR5154] ab109367)
Predicted band size: 22 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peroxiredoxin 2/PRP antibody [EPR5154] - BSA and Azide free (ab227988)
This IHC data was generated using the same anti-Peroxiredoxin 2/PRP antibody clone, EPR5154, in a different buffer formulation (cat# Anti-Peroxiredoxin 2/PRP antibody [EPR5154] ab109367).
Immunohistochemical staining of paraffin embedded human endometrial carcinoma with purified Anti-Peroxiredoxin 2/PRP antibody [EPR5154] ab109367 at a working dilution of 1/500. The secondary antibody used is Goat Anti-Rabbit IgG H&L (HRP) ab97051, a goat anti-rabbit IgG (H&L) at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
Immunocytochemistry/ Immunofluorescence - Anti-Peroxiredoxin 2/PRP antibody [EPR5154] - BSA and Azide free (ab227988)
Immunofluorescence staining of HeLa cells with purified Anti-Peroxiredoxin 2/PRP antibody [EPR5154] ab109367 at a working dilution of 1/100, counter-stained with DAPI. The secondary antibody was Alexa Fluor^® 488 goat anti-rabbit (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077), used at a dilution of 1/1000. Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291, a mouse anti-tubulin antibody (1/1000), was used to stain tubulin along with Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 (Alexa Fluor^® 594 goat anti-mouse, 1/1000), shown in the top right hand panel. The cells were fixed in 100% methanol and permeabilized using 0.1% Triton X 100. The negative controls are shown in bottom middle and right hand panels - for negative control 1, purified Anti-Peroxiredoxin 2/PRP antibody [EPR5154] ab109367 was used at a dilution of 1/500 followed by an Alexa Fluor^® 594 goat anti-mouse antibody (Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120) at a dilution of 1/500. For negative control 2, Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (mouse anti-tubulin) was used at a dilution of 1/500 followed by an Alexa Fluor^® 488 goat anti-rabbit antibody (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at a dilution of 1/400.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Peroxiredoxin 2/PRP antibody [EPR5154] ab109367).
Western blot - Anti-Peroxiredoxin 2/PRP antibody [EPR5154] - BSA and Azide free (ab227988)
This data was developed using the same antibody clone in a different buffer formulation (Anti-Peroxiredoxin 2/PRP antibody [EPR5154] ab109367).
Lanes 1- 2: Merged signal (red and green). Green - Anti-Peroxiredoxin 2/PRP antibody [EPR5154] ab109367 observed at 22 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) observed at 37 kDa.
Anti-Peroxiredoxin 2/PRP antibody [EPR5154] ab109367 was shown to react with Peroxiredoxin 2/PRP in wild-type HEK-293T cells in western blot. Loss of signal was observed when knockout cell line Human PRDX2 (Peroxiredoxin 2/PRP) knockout HEK-293T cell line ab266392 (knockout cell lysate Human PRDX2 (Peroxiredoxin 2/PRP) knockout HEK-293T cell lysate ab257041) was used. Wild-type HEK-293T and PRDX2 knockout HEK-293T cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. Anti-Peroxiredoxin 2/PRP antibody [EPR5154] ab109367 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^®800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye^®680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-Peroxiredoxin 2/PRP antibody [EPR5154] (Anti-Peroxiredoxin 2/PRP antibody [EPR5154] ab109367) at 1/1000 dilution
Lane 1: Wild-type HEK-293T cell lysate at 20 µg
Lane 2: PRDX2 knockout HEK-293T cell lysate at 20 µg
Lane 2: Western blot - Human PRDX2 (Peroxiredoxin 2/PRP) knockout HEK-293T cell line (Human PRDX2 (Peroxiredoxin 2/PRP) knockout HEK-293T cell line ab266392)
Performed under reducing conditions.
Predicted band size: 22 kDa
Observed band size: 22 kDa
Flow Cytometry (Intracellular) - Anti-Peroxiredoxin 2/PRP antibody [EPR5154] - BSA and Azide free (ab227988)
Overlay histogram showing HeLa cells fixed in 80% methanol and stained with purified Anti-Peroxiredoxin 2/PRP antibody [EPR5154] ab109367 at a dilution of 1/200 (red line). The secondary antibody used was Alexa Fluor^® 488 goat anti-rabbit at a dilution of 1/500. Rabbit monoclonal IgG was used as an isotype control (black line) and cells incubated in the absence of both primary and secondary antibody were used as a negative control (blue line).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Peroxiredoxin 2/PRP antibody [EPR5154] ab109367).
Flow Cytometry (Intracellular) - Anti-Peroxiredoxin 2/PRP antibody [EPR5154] - BSA and Azide free (ab227988)
Overlay histogram showing HeLa cells stained with unpurified Anti-Peroxiredoxin 2/PRP antibody [EPR5154] ab109367 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (Anti-Peroxiredoxin 2/PRP antibody [EPR5154] ab109367, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor^® 488 goat anti-rabbit IgG (H+L) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x10⁶ cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Peroxiredoxin 2/PRP antibody [EPR5154] ab109367).
Immunocytochemistry/ Immunofluorescence - Anti-Peroxiredoxin 2/PRP antibody [EPR5154] - BSA and Azide free (ab227988)
Immunofluorescent staining of Peroxiredoxin 2/PRP in HeLa cells using unpurified Anti-Peroxiredoxin 2/PRP antibody [EPR5154] ab109367 at 1/250 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Peroxiredoxin 2/PRP antibody [EPR5154] ab109367).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peroxiredoxin 2/PRP antibody [EPR5154] - BSA and Azide free (ab227988)
Immunohistochemical analysis of Peroxiredoxin 2/PRP in paraffin-embedded Human prostatic hyperplasia tissue using unpurified Anti-Peroxiredoxin 2/PRP antibody [EPR5154] ab109367 at 1/1000 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Peroxiredoxin 2/PRP antibody [EPR5154] ab109367).
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peroxiredoxin 2/PRP antibody [EPR5154] - BSA and Azide free (ab227988)
Immunohistochemical analysis of Peroxiredoxin 2/PRP in paraffin-embedded Human stomach tissue using unpurified Anti-Peroxiredoxin 2/PRP antibody [EPR5154] ab109367 at 1/1000 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Peroxiredoxin 2/PRP antibody [EPR5154] ab109367).
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Image courtesy of a customer review submitted by Dr. Kirk McManus, Univ. of Manitoba/Cancer Care MICB, Canada
Immunocytochemistry/ Immunofluorescence - Anti-Peroxiredoxin 2/PRP antibody [EPR5154] - BSA and Azide free (ab227988)
Unpurified Anti-Peroxiredoxin 2/PRP antibody [EPR5154] ab109367 (1/500) staining Peroxiredoxin 2/PRP in asynchronous HeLa cells (green). Cells were fixed in methanol and counterstained with DAPI in order to highlight the nucleus (red). For further experimental details please refer to Abreview.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Peroxiredoxin 2/PRP antibody [EPR5154] ab109367).