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Rabbit Recombinant Monoclonal Peroxiredoxin 2/PRP antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 4 publications.


Images

Western blot - Anti-Peroxiredoxin 2/PRP antibody [EPR5155] (AB133481), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-Peroxiredoxin 2/PRP antibody [EPR5155] (AB133481), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Peroxiredoxin 2/PRP antibody [EPR5155] (AB133481), expandable thumbnail
  • Western blot - Anti-Peroxiredoxin 2/PRP antibody [EPR5155] (AB133481), expandable thumbnail
  • Western blot - Anti-Peroxiredoxin 2/PRP antibody [EPR5155] (AB133481), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBICC/IFFlow Cyt (Intra)IHC-P
Human
Tested
Tested
Tested
Not recommended
Mouse
Expected
Expected
Expected
Not recommended
Rat
Expected
Predicted
Predicted
Not recommended

Tested
Tested

Species
Human
Dilution info
1/50000 - 1/200000
Notes

-

Expected
Expected

Species
Mouse
Dilution info
1/50000 - 1/200000
Notes

-

Species
Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/100 - 1/250
Notes

-

Expected
Expected

Species
Mouse
Dilution info
1/100 - 1/250
Notes

-

Predicted
Predicted

Species
Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/100 - 1/1000
Notes

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Expected
Expected

Species
Mouse
Dilution info
1/100 - 1/1000
Notes

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Predicted
Predicted

Species
Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Human, Rat
Dilution info
-
Notes

-

Associated Products

Select an associated product type

6 products for Alternative Product

Target data

Function

Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively. Plays a role in cell protection against oxidative stress by detoxifying peroxides and as sensor of hydrogen peroxide-mediated signaling events. Might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H(2)O(2).

Alternative names

NKEFB, TDPX1, PRDX2, Peroxiredoxin-2, Natural killer cell-enhancing factor B, PRP, Thiol-specific antioxidant protein, Thioredoxin peroxidase 1, Thioredoxin-dependent peroxide reductase 1, Thioredoxin-dependent peroxiredoxin 2, NKEF-B, TSA

Recommended products

Rabbit Recombinant Monoclonal Peroxiredoxin 2/PRP antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 4 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR5155
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Peroxiredoxin 2 (also known as PRP or PRDX2) is an antioxidant enzyme that plays an important role in reducing peroxides and protecting cells from oxidative damage. The target consisting of approximately 22kDa mass is expressed heavily in erythrocytes but can also be found in other tissues like the heart and liver. PRDX2 belongs to the peroxiredoxin family and its redox activity contributes significantly to cellular homeostasis and defense against oxidative stress.

Biological function summary

Peroxiredoxins like PRDX2 function by breaking down hydrogen peroxide and organic hydroperoxides safeguarding cells from oxidative harm. PRDX2 often forms homodimers or higher-order oligomers which influence its catalytic efficiency and chaperone activity. Within the cell it not only acts independently but also associates with other cellular components reflecting its participation in important cellular processes including cell proliferation and apoptosis.

Pathways

PRDX2 engages significantly within antioxidant defense systems specifically the thioredoxin pathway. It interacts with thioredoxin reductase and thioredoxin to facilitate the reduction of peroxides maintaining the cell’s redox balance. Furthermore PRDX2 intersects with cellular signaling pathways associated with inflammation and the regulation of cell death where proteins like NRF2 and KEAP1 play important roles in managing oxidant-inducible gene expression.

Associated diseases and disorders

Disruptions in PRDX2 function connect to conditions like cancer and cardiovascular diseases. In cancer altered peroxiredoxin 2 expression correlates to tumor progression and resistance to chemotherapy implicating its interaction with proteins like NF-κB in enhancing cell survival. In cardiovascular diseases oxidative stress mediated by PRDX2 imbalance can lead to myocardial infarction and heart failure linking it to proteins such as SOD2 involved in mitochondrial defense.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Western blot - Anti-Peroxiredoxin 2/PRP antibody [EPR5155] (ab133481), expandable thumbnail

    Western blot - Anti-Peroxiredoxin 2/PRP antibody [EPR5155] (ab133481)

    Lane 1: Wild-type HAP1 cell lysate (20 μg)
    Lane 2: Peroxiredoxin 2/PRP knockout HAP1 cell lysate (20 μg)
    Lane 3: HeLa cell lysate (20 μg)
    Lane 4: LnCaP cell lysate (20 μg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab133481 observed at 24 kDa. Red - loading control, Anti-GAPDH antibody [6C5] - Loading Control ab8245, observed at 37 kDa.
    ab133481 was shown to specifically react with Peroxiredoxin 2/PRP when Peroxiredoxin 2/PRP knockout samples were used. Wild-type and Peroxiredoxin 2/PRP knockout samples were subjected to SDS-PAGE. ab133481 and Anti-GAPDH antibody [6C5] - Loading Control ab8245 (loading control to GAPDH) were diluted 1/50 000 and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773)
    and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.

    All lanes: Western blot - Anti-Peroxiredoxin 2/PRP antibody [EPR5155] (ab133481)

    Predicted band size: 22 kDa

  • Flow Cytometry (Intracellular) - Anti-Peroxiredoxin 2/PRP antibody [EPR5155] (ab133481), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-Peroxiredoxin 2/PRP antibody [EPR5155] (ab133481)

    Overlay histogram showing HeLa cells stained with ab133481 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab133481, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

  • Immunocytochemistry/ Immunofluorescence - Anti-Peroxiredoxin 2/PRP antibody [EPR5155] (ab133481), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Peroxiredoxin 2/PRP antibody [EPR5155] (ab133481)

    Immunofluorescence analysis of HeLa cells labelling Peroxiredoxin 2/PRP with ab133481 at 1/100 dilution.

  • Western blot - Anti-Peroxiredoxin 2/PRP antibody [EPR5155] (ab133481), expandable thumbnail

    Western blot - Anti-Peroxiredoxin 2/PRP antibody [EPR5155] (ab133481)

    Lanes 1- 2: Merged signal (red and green). Green - ab133481 observed at 22 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) observed at 37 kDa.

    ab133481 was shown to react with Peroxiredoxin 2/PRP in wild-type HEK-293T cells in western blot. Loss of signal was observed when knockout cell line Human PRDX2 (Peroxiredoxin 2/PRP) knockout HEK-293T cell line ab266392 (knockout cell lysate Human PRDX2 (Peroxiredoxin 2/PRP) knockout HEK-293T cell lysate ab257041) was used. Wild-type HEK-293T and PRDX2 knockout HEK-293T cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab133481 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) overnight at 4°C at a 1 in 5000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-Peroxiredoxin 2/PRP antibody [EPR5155] (ab133481) at 1/5000 dilution

    Lane 1: Wild-type HEK-293T cell lysate at 20 µg

    Lane 2: PRDX2 knockout HEK-293T cell lysate at 20 µg

    Lane 2: Western blot - Human PRDX2 (Peroxiredoxin 2/PRP) knockout HEK-293T cell line (Human PRDX2 (Peroxiredoxin 2/PRP) knockout HEK-293T cell line ab266392)

    Performed under reducing conditions.

    Predicted band size: 22 kDa

    Observed band size: 22 kDa

  • Western blot - Anti-Peroxiredoxin 2/PRP antibody [EPR5155] (ab133481), expandable thumbnail

    Western blot - Anti-Peroxiredoxin 2/PRP antibody [EPR5155] (ab133481)

    All lanes: Western blot - Anti-Peroxiredoxin 2/PRP antibody [EPR5155] (ab133481) at 1/50000 dilution

    Lane 1: 293T cell lysate at 10 µg

    Lane 2: HepG2 cell lysate at 10 µg

    Lane 3: LnCaP cell lysate at 10 µg

    Lane 4: U937 cell lysate at 10 µg

    Secondary

    All lanes: HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 22 kDa

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