Rabbit Polyclonal Peroxiredoxin 3/PRDX3 antibody. Suitable for IP, WB, IHC-P, ICC/IF and reacts with Human, Mouse samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human PRDX3 aa 50 to C-terminus.
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- Science advances 7:eabl49882021Skeletal muscle NOX4 is required for adaptive responses that prevent insulin resistance.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChrysovalantou E Xirouchaki,Yaoyao Jia,Meagan J McGrath,Spencer Greatorex,Melanie Tran,Troy L Merry,Dawn Hong,Matthew J Eramo,Sophie C Broome,Jonathan S T Woodhead,Randall F D'souza,Jenny Gallagher,Ekaterina Salimova,Cheng Huang,Ralf B Schittenhelm,Junichi Sadoshima,Matthew J Watt,Christina A Mitchell,Tony TiganisPubMed 34910515
- PLoS biology 19:e30012522021Generation of mitochondrial reactive oxygen species is controlled by ATPase inhibitory factor 1 and regulates cognition.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPau B Esparza-Moltó,Inés Romero-Carramiñana,Cristina Núñez de Arenas,Marta P Pereira,Noelia Blanco,Beatriz Pardo,Georgina R Bates,Carla Sánchez-Castillo,Rafael Artuch,Michael P Murphy,José A Esteban,José M CuezvaPubMed 33983919
- Cancer management and research 11:9685-96992019The Prognosis Of Peroxiredoxin Family In Breast Cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGang Wang et. al.PubMed 31814764
- Biochemical and biophysical research communications 508:805-8102018The association of plasma peroxiredoxin 3 with insulin in pregnant women.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLi Wang,Jun-Mei Hao,Ai-Qun Yu,Tian-Tian Li,Ran-Ran Liu,Lianqin Li,Jingmin Li,Xiaoyan LiPubMed 30528738
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.03% Proclin 300
Constituents: PBS, 50% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human PRDX3 aa 50 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P30048
Reactivity data
Select an application| IP | WB | IHC-P | ICC/IF | |
|---|---|---|---|---|
| Human | Tested | Tested | Tested | Tested |
| Mouse | Expected | Tested | Expected | Expected |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/200.00000 - 1/2000.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/5000.00000 | Notes - |
Species Mouse | Dilution info 1/500.00000 - 1/5000.00000 | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/20.00000 - 1/200.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50.00000 - 1/200.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
4 products for Alternative Product
Target data
Function
Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively. Plays a role in cell protection against oxidative stress by detoxifying peroxides (PubMed:17707404, PubMed:29438714, PubMed:33889951, PubMed:7733872). Acts synergistically with MAP3K13 to regulate the activation of NF-kappa-B in the cytosol (PubMed:12492477). Required for the maintenance of physical strength (By similarity).
Alternative names
AOP1, PRDX3, Antioxidant protein 1, HBC189, Peroxiredoxin III, Peroxiredoxin-3, Protein MER5 homolog, Thioredoxin-dependent peroxiredoxin 3, AOP-1, Prx-III
Recommended products
Rabbit Polyclonal Peroxiredoxin 3/PRDX3 antibody. Suitable for IP, WB, IHC-P, ICC/IF and reacts with Human, Mouse samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human PRDX3 aa 50 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.03% Proclin 300
Constituents: PBS, 50% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human PRDX3 aa 50 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P30048
- Purification technique
- Affinity purification Protein G
- Concentration
- Purification notes
Purity >95%.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Peroxiredoxin 3 also known as PRDX3 is an antioxidant enzyme that plays a critical role in the reduction of hydrogen peroxide to water. This protein has a mass of around 25 kDa and belongs to the thioredoxin peroxidase family. It functions within the mitochondria and is expressed in a wide variety of tissues with higher levels in the heart liver and kidney. PRDX3 acts as a protective buffer against oxidative stress by maintaining redox balance in cells.
- Biological function summary
The enzyme helps to safeguard cells from oxidative damage by reducing peroxides produced during cellular metabolism. PRDX3 participates in antioxidant defense mechanisms and may form functional complexes with other proteins to enhance its peroxidase activity. Its antioxidant properties contribute to the maintenance of mitochondrial integrity and influence cellular survival and apoptosis processes.
- Pathways
PRDX3 integrates into the cellular antioxidant defense system prominently in the thioredoxin pathway. In this pathway PRDX3 synergizes with thioredoxin to neutralize harmful oxidative molecules. PRDX3's activity is interconnected with proteins such as thioredoxin and glutaredoxin which also partake in counteracting oxidative stress bolstering an organism's defense against cellular damage.
- Associated diseases and disorders
Alterations in PRDX3 activity associate with conditions like cancer and neurodegenerative disorders. Overexpression of PRDX3 has been observed in certain types of cancer suggesting a potential role in tumor growth through its influence on cell proliferation and survival. In neurodegenerative diseases dysfunctional PRDX3 contributes to neuronal injury. It interacts with proteins like DJ-1 which relate to pathways involved in Parkinson's disease indicating its impact on maintaining neuronal health.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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5 product images
Western blot - Anti-Peroxiredoxin 3/PRDX3 antibody (ab222807)
All lanes: Western blot - Anti-Peroxiredoxin 3/PRDX3 antibody (ab222807) at 1/500 dilution
Lane 1: HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2: HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 3: MCF7 (human breast adenocarcinoma cell line) whole cell lysate
Lane 4: Mouse heart lysate
Lane 5: Mouse liver lysate
Lane 6: Mouse brain lysate
Lane 7: Mouse lung lysate
Lane 8: Mouse kidney lysate
Lane 9: Mouse stomach lysate
SecondaryAll lanes: Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 28 kDa
Immunocytochemistry/ Immunofluorescence - Anti-Peroxiredoxin 3/PRDX3 antibody (ab222807)
HepG2 (human liver hepatocellular carcinoma cell line) cells stained for Peroxiredoxin 3/PRDX3 (green) using ab222807 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peroxiredoxin 3/PRDX3 antibody (ab222807)
Paraffin-embedded human renal tissue stained for Peroxiredoxin 3/PRDX3 using ab222807 at 1/100 dilution in immunohistochemical analysis.
Immunoprecipitation - Anti-Peroxiredoxin 3/PRDX3 antibody (ab222807)
Peroxiredoxin 3/PRDX3 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab222807 at 1/200 dilution. Western blot was performed from the immunoprecipitate using ab222807, followed by HRP-conjugated Protein G secondary antibody at 1/2000 dilution.
Lane 1: Rabbit monoclonal IgG (1 μg) instead of ab222807 in Hela whole cell lysate.
Lane 2: ab222807 IP in Hela whole cell lysate (500 μg).
Lane 3: Hela whole cell lysate (10 μg) (Input).
All lanes: Immunoprecipitation - Anti-Peroxiredoxin 3/PRDX3 antibody (ab222807)
Predicted band size: 28 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peroxiredoxin 3/PRDX3 antibody (ab222807)
Paraffin-embedded human lung cancer tissue stained for Peroxiredoxin 3/PRDX3 using ab222807 at 1/100 dilution in immunohistochemical analysis.
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