Rabbit Recombinant Monoclonal Peroxiredoxin 3/PRDX3 antibody. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 2 publications.
Images
![Western blot - Anti-Peroxiredoxin 3/PRDX3 antibody [EPR8114] (AB129206), expandable thumbnail](https://content.abcam.com/products/images/peroxiredoxin-3-prdx3-antibody-epr8114-ab129206--western-blot-img104289.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Peroxiredoxin 3/PRDX3 antibody [EPR8114] (AB129206)")
![Immunocytochemistry/ Immunofluorescence - Anti-Peroxiredoxin 3/PRDX3 antibody [EPR8114] (AB129206), expandable thumbnail](https://content.abcam.com/products/images/peroxiredoxin-3-prdx3-antibody-epr8114-ab129206--immunocytochemistry-immunofluorescence-img89060.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Peroxiredoxin 3/PRDX3 antibody [EPR8114] (AB129206)")
![Flow Cytometry (Intracellular) - Anti-Peroxiredoxin 3/PRDX3 antibody [EPR8114] (AB129206), expandable thumbnail](https://content.abcam.com/products/images/peroxiredoxin-3-prdx3-antibody-epr8114-ab129206--flow-cytometry-intracellular-img25886.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-Peroxiredoxin 3/PRDX3 antibody [EPR8114] (AB129206)")
![Western blot - Anti-Peroxiredoxin 3/PRDX3 antibody [EPR8114] (AB129206), expandable thumbnail](https://content.abcam.com/products/images/peroxiredoxin-3-prdx3-antibody-epr8114-ab129206--western-blot-img33713.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Peroxiredoxin 3/PRDX3 antibody [EPR8114] (AB129206)")
Publications
Filter by
- mBio 10:2019A Genome-Wide Knockout Screen in Human Macrophages Identified Host Factors Modulating Infection.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAmy T Y Yeung et. al.PubMed 31594818
- Endocrine-related cancer 25:943-9542018A unique model for SDH-deficient GIST: an endocrine-related cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJames F Powers,Brent Cochran,James D Baleja,Hadley D Sikes,Xue Zhang,Inna Lomakin,Troy Langford,Kassi Taylor Stein,Arthur S TischlerPubMed 29967109
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | IP | WB | ICC/IF | Flow Cyt (Intra) | |
|---|---|---|---|---|---|
| Human | Tested | Not recommended | Tested | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50 - 1/100 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50000 - 1/200000 | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50 - 1/100 | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
4 products for Alternative Product
Target data
Function
Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively. Plays a role in cell protection against oxidative stress by detoxifying peroxides (PubMed:17707404, PubMed:29438714, PubMed:33889951, PubMed:7733872). Acts synergistically with MAP3K13 to regulate the activation of NF-kappa-B in the cytosol (PubMed:12492477). Required for the maintenance of physical strength (By similarity).
Alternative names
AOP1, PRDX3, Antioxidant protein 1, HBC189, Peroxiredoxin III, Peroxiredoxin-3, Protein MER5 homolog, Thioredoxin-dependent peroxiredoxin 3, AOP-1, Prx-III
Recommended products
Rabbit Recombinant Monoclonal Peroxiredoxin 3/PRDX3 antibody. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 2 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR8114
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Stable for 12 months at -20°C
Notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Peroxiredoxin 3 also known as PRDX3 is an antioxidant enzyme that plays a critical role in the reduction of hydrogen peroxide to water. This protein has a mass of around 25 kDa and belongs to the thioredoxin peroxidase family. It functions within the mitochondria and is expressed in a wide variety of tissues with higher levels in the heart liver and kidney. PRDX3 acts as a protective buffer against oxidative stress by maintaining redox balance in cells.
- Biological function summary
The enzyme helps to safeguard cells from oxidative damage by reducing peroxides produced during cellular metabolism. PRDX3 participates in antioxidant defense mechanisms and may form functional complexes with other proteins to enhance its peroxidase activity. Its antioxidant properties contribute to the maintenance of mitochondrial integrity and influence cellular survival and apoptosis processes.
- Pathways
PRDX3 integrates into the cellular antioxidant defense system prominently in the thioredoxin pathway. In this pathway PRDX3 synergizes with thioredoxin to neutralize harmful oxidative molecules. PRDX3's activity is interconnected with proteins such as thioredoxin and glutaredoxin which also partake in counteracting oxidative stress bolstering an organism's defense against cellular damage.
- Associated diseases and disorders
Alterations in PRDX3 activity associate with conditions like cancer and neurodegenerative disorders. Overexpression of PRDX3 has been observed in certain types of cancer suggesting a potential role in tumor growth through its influence on cell proliferation and survival. In neurodegenerative diseases dysfunctional PRDX3 contributes to neuronal injury. It interacts with proteins like DJ-1 which relate to pathways involved in Parkinson's disease indicating its impact on maintaining neuronal health.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
6 product images
![Western blot - Anti-Peroxiredoxin 3/PRDX3 antibody [EPR8114] (ab129206), expandable thumbnail](https://content.abcam.com/products/images/peroxiredoxin-3-prdx3-antibody-epr8114-ab129206--western-blot-img104289.jpg "Western blot - Anti-Peroxiredoxin 3/PRDX3 antibody [EPR8114] (ab129206)")
Western blot - Anti-Peroxiredoxin 3/PRDX3 antibody [EPR8114] (ab129206)
Lane 1: Wild-type HAP1 cell lysate (20 μg)
Lane 2: Peroxiredoxin 3/PRDX3 knockout HAP1 cell lysate (20 μg)
Lane 3: MCF7 cell lysate (20 μg)
Lane 4: A431 cell lysate (20 μg)
Lanes 1 - 4: Merged signal (red and green). Green - ab129206 observed at 24 kDa. Red - loading control, Anti-GAPDH antibody [6C5] - Loading Control ab8245, observed at 37 kDa.
ab129206 was shown to specifically react with Peroxiredoxin 3/PRDX3 when Peroxiredoxin 3/PRDX3 knockout samples were used. Wild-type and Peroxiredoxin 3/PRDX3 knockout samples were subjected to SDS-PAGE. ab129206 and Anti-GAPDH antibody [6C5] - Loading Control ab8245 (loading control to GAPDH) were both diluted 1/50000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.All lanes: Western blot - Anti-Peroxiredoxin 3/PRDX3 antibody [EPR8114] (ab129206)
Predicted band size: 28 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-Peroxiredoxin 3/PRDX3 antibody [EPR8114] (ab129206), expandable thumbnail](https://content.abcam.com/products/images/peroxiredoxin-3-prdx3-antibody-epr8114-ab129206--immunocytochemistry-immunofluorescence-img89060.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Peroxiredoxin 3/PRDX3 antibody [EPR8114] (ab129206)")
Immunocytochemistry/ Immunofluorescence - Anti-Peroxiredoxin 3/PRDX3 antibody [EPR8114] (ab129206)
ab129206, at 1/50 dilution, staining Peroxiredoxin 3/PRDX3 in MCF-7 cells by Immunofluorescence.
![Flow Cytometry (Intracellular) - Anti-Peroxiredoxin 3/PRDX3 antibody [EPR8114] (ab129206), expandable thumbnail](https://content.abcam.com/products/images/peroxiredoxin-3-prdx3-antibody-epr8114-ab129206--flow-cytometry-intracellular-img25886.jpg "Flow Cytometry (Intracellular) - Anti-Peroxiredoxin 3/PRDX3 antibody [EPR8114] (ab129206)")
Flow Cytometry (Intracellular) - Anti-Peroxiredoxin 3/PRDX3 antibody [EPR8114] (ab129206)
Intracellular Flow Cytometry analysis of MCF-7 (human breast carcinoma) cells labeling Peroxiredoxin 3/PRDX3 with unpurified ab129206 at 1/150 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluorr® 488) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.
![Western blot - Anti-Peroxiredoxin 3/PRDX3 antibody [EPR8114] (ab129206), expandable thumbnail](https://content.abcam.com/products/images/peroxiredoxin-3-prdx3-antibody-epr8114-ab129206--western-blot-img33713.jpg "Western blot - Anti-Peroxiredoxin 3/PRDX3 antibody [EPR8114] (ab129206)")
Western blot - Anti-Peroxiredoxin 3/PRDX3 antibody [EPR8114] (ab129206)
All lanes: Western blot - Anti-Peroxiredoxin 3/PRDX3 antibody [EPR8114] (ab129206) at 1/50000 dilution
Lane 1: HeLa cell lysate at 10 µg
Lane 2: 293T (Human embryonic kidney epithelial cell) cell lysate at 10 µg
Lane 3: MCF7 cell lysate at 10 µg
Lane 4: A431 cell lysate at 10 µg
SecondaryAll lanes: Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 28 kDa
Observed band size: 26 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peroxiredoxin 3/PRDX3 antibody [EPR8114] (ab129206), expandable thumbnail](https://content.abcam.com/products/images/peroxiredoxin-3-prdx3-antibody-epr8114-ab129206--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img137646.gif "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peroxiredoxin 3/PRDX3 antibody [EPR8114] (ab129206)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peroxiredoxin 3/PRDX3 antibody [EPR8114] (ab129206)
ab129206, at 1/50 dilution, staining Peroxiredoxin 3/PRDX3 in paraffin-embedded Human liver tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peroxiredoxin 3/PRDX3 antibody [EPR8114] (ab129206), expandable thumbnail](https://content.abcam.com/products/images/peroxiredoxin-3-prdx3-antibody-epr8114-ab129206--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img74190.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peroxiredoxin 3/PRDX3 antibody [EPR8114] (ab129206)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peroxiredoxin 3/PRDX3 antibody [EPR8114] (ab129206)
ab129206, at 1/50 dilution, staining Peroxiredoxin 3/PRDX3 in paraffin-embedded Human thyroid gland carcinoma tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peroxiredoxin 3/PRDX3 antibody [EPR8114] (ab129206), expandable thumbnail](https://content.abcam.com/products/images/peroxiredoxin-3-prdx3-antibody-epr8114-ab129206--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img74190.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peroxiredoxin 3/PRDX3 antibody [EPR8114] (ab129206)")
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