Skip to main content

Rabbit Recombinant Monoclonal Peroxiredoxin 3/PRDX3 antibody. Suitable for IHC-P, IP, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 8 publications.


Images

Western blot - Anti-Peroxiredoxin 3/PRDX3 antibody [EPR8115] (AB128953), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peroxiredoxin 3/PRDX3 antibody [EPR8115] (AB128953), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-Peroxiredoxin 3/PRDX3 antibody [EPR8115] (AB128953), expandable thumbnail
  • Immunoprecipitation - Anti-Peroxiredoxin 3/PRDX3 antibody [EPR8115] (AB128953), expandable thumbnail
  • Western blot - Anti-Peroxiredoxin 3/PRDX3 antibody [EPR8115] (AB128953), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PIPWBFlow Cyt (Intra)
Human
Tested
Tested
Tested
Tested

Tested
Tested

Species
Human
Dilution info
1/250 - 1/500
Notes

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Tested
Tested

Species
Human
Dilution info
1/100 - 1/500
Notes

-

Tested
Tested

Species
Human
Dilution info
1/10000 - 1/50000
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Associated Products

Select an associated product type

4 products for Alternative Product

Target data

Function

Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively. Plays a role in cell protection against oxidative stress by detoxifying peroxides (PubMed:17707404, PubMed:29438714, PubMed:33889951, PubMed:7733872). Acts synergistically with MAP3K13 to regulate the activation of NF-kappa-B in the cytosol (PubMed:12492477). Required for the maintenance of physical strength (By similarity).

Alternative names

AOP1, PRDX3, Antioxidant protein 1, HBC189, Peroxiredoxin III, Peroxiredoxin-3, Protein MER5 homolog, Thioredoxin-dependent peroxiredoxin 3, AOP-1, Prx-III

Recommended products

Rabbit Recombinant Monoclonal Peroxiredoxin 3/PRDX3 antibody. Suitable for IHC-P, IP, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 8 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR8115
Purification technique
Affinity purification Protein A
Dissociation constant
1.02 x 10-11 M
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C

Notes

Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Peroxiredoxin 3 also known as PRDX3 is an antioxidant enzyme that plays a critical role in the reduction of hydrogen peroxide to water. This protein has a mass of around 25 kDa and belongs to the thioredoxin peroxidase family. It functions within the mitochondria and is expressed in a wide variety of tissues with higher levels in the heart liver and kidney. PRDX3 acts as a protective buffer against oxidative stress by maintaining redox balance in cells.

Biological function summary

The enzyme helps to safeguard cells from oxidative damage by reducing peroxides produced during cellular metabolism. PRDX3 participates in antioxidant defense mechanisms and may form functional complexes with other proteins to enhance its peroxidase activity. Its antioxidant properties contribute to the maintenance of mitochondrial integrity and influence cellular survival and apoptosis processes.

Pathways

PRDX3 integrates into the cellular antioxidant defense system prominently in the thioredoxin pathway. In this pathway PRDX3 synergizes with thioredoxin to neutralize harmful oxidative molecules. PRDX3's activity is interconnected with proteins such as thioredoxin and glutaredoxin which also partake in counteracting oxidative stress bolstering an organism's defense against cellular damage.

Associated diseases and disorders

Alterations in PRDX3 activity associate with conditions like cancer and neurodegenerative disorders. Overexpression of PRDX3 has been observed in certain types of cancer suggesting a potential role in tumor growth through its influence on cell proliferation and survival. In neurodegenerative diseases dysfunctional PRDX3 contributes to neuronal injury. It interacts with proteins like DJ-1 which relate to pathways involved in Parkinson's disease indicating its impact on maintaining neuronal health.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

7 product images

  • Western blot - Anti-Peroxiredoxin 3/PRDX3 antibody [EPR8115] (ab128953), expandable thumbnail

    Western blot - Anti-Peroxiredoxin 3/PRDX3 antibody [EPR8115] (ab128953)

    Lane 1: Wild-type HAP1 cell lysate (20 μg)
    Lane 2: Peroxiredoxin 3/PRDX3 knockout HAP1 cell lysate (20 μg)
    Lane 3: MCF7 cell lysate (20 μg)
    Lane 4: A431 cell lysate (20 μg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab128953 observed at 24 kDa. Red - loading control, Anti-GAPDH antibody [6C5] - Loading Control ab8245, observed at 37 kDa.


    ab128953 was shown to specifically react with Peroxiredoxin 3/PRDX3 in wild-type HAP1 cells. No band was observed when Peroxiredoxin 3/PRDX3 knockout samples were examined. Wild-type and Peroxiredoxin 3/PRDX3 knockout samples were subjected to SDS-PAGE. ab128953 and Anti-GAPDH antibody [6C5] - Loading Control ab8245 (loading control to GAPDH) were both diluted 1/10,000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-Peroxiredoxin 3/PRDX3 antibody [EPR8115] (ab128953)

    Predicted band size: 28 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peroxiredoxin 3/PRDX3 antibody [EPR8115] (ab128953), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peroxiredoxin 3/PRDX3 antibody [EPR8115] (ab128953)

    ab128953, at a 1/250 dilution, staining Peroxiredoxin 3/PRDX3 in paraffin embedded Human endometrium adenocarcinoma tissue by Immunohistochemistry.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Flow Cytometry (Intracellular) - Anti-Peroxiredoxin 3/PRDX3 antibody [EPR8115] (ab128953), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-Peroxiredoxin 3/PRDX3 antibody [EPR8115] (ab128953)

    Flow Cytometry analysis of MCF7 (Human breast adenocarcinoma epithelial cell) cells labeling Peroxiredoxin 3/PRDX3 with purified ab128953 at 1/1000 dilution (1 μg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

  • Immunoprecipitation - Anti-Peroxiredoxin 3/PRDX3 antibody [EPR8115] (ab128953), expandable thumbnail

    Immunoprecipitation - Anti-Peroxiredoxin 3/PRDX3 antibody [EPR8115] (ab128953)

    Peroxiredoxin 3/PRDX3 was immunoprecipitated from 0.35 mg HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10 μg with 128953 at 1/50 dilution (2μg). VeriBlot for IP Detection Reagent (HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.

    Lane 1: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10 μg

    Lane 2: ab128953 IP in HeLa whole cell lysate

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab128953 in HeLa whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Upper bands could be ubiquitinated forms (PMID:21795677)

    All lanes: Immunoprecipitation - Anti-Peroxiredoxin 3/PRDX3 antibody [EPR8115] (ab128953)

    Predicted band size: 28 kDa

    Observed band size: 26 kDa

  • Western blot - Anti-Peroxiredoxin 3/PRDX3 antibody [EPR8115] (ab128953), expandable thumbnail

    Western blot - Anti-Peroxiredoxin 3/PRDX3 antibody [EPR8115] (ab128953)

    All lanes: Western blot - Anti-Peroxiredoxin 3/PRDX3 antibody [EPR8115] (ab128953) at 1/10000 dilution

    Lane 1: HeLa cell lysate at 10 µg

    Lane 2: 293T (Human embryonic kidney epithelial cell) cell lysate at 10 µg

    Lane 3: MCF7 cell lysate at 10 µg

    Lane 4: A431 cell lysate at 10 µg

    Secondary

    All lanes: HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 28 kDa

    Observed band size: 26 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peroxiredoxin 3/PRDX3 antibody [EPR8115] (ab128953), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peroxiredoxin 3/PRDX3 antibody [EPR8115] (ab128953)

    ab128953, at a 1/250 dilution, staining Peroxiredoxin 3/PRDX3 in paraffin embedded Human liver tissue by Immunohistochemistry.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • OI-RD Scanning - Anti-Peroxiredoxin 3/PRDX3 antibody [EPR8115] (ab128953), expandable thumbnail

    OI-RD Scanning - Anti-Peroxiredoxin 3/PRDX3 antibody [EPR8115] (ab128953)

    We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
    Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com